香紫苏油主成分含量测定及其香气贡献评价

白冰; 楚首道; 杨靖; 张改红; 贾春晓; 毛多斌

doi:10.3969/j.issn.2096-1553.2018.01.002

CN 41-1437/TS ISSN 2096-1553

香紫苏油主成分含量测定及其香气贡献评价

白冰 , 楚首道 , 杨靖 , 张改红 , 贾春晓 , 毛多斌

白冰, 楚首道, 杨靖, 等. 香紫苏油主成分含量测定及其香气贡献评价[J]. 轻工学报, 2018, 33(1): 7-12. doi: 10.3969/j.issn.2096-1553.2018.01.002

引用本文: 白冰, 楚首道, 杨靖, 等. 香紫苏油主成分含量测定及其香气贡献评价[J]. 轻工学报, 2018, 33(1): 7-12. doi: 10.3969/j.issn.2096-1553.2018.01.002

BAI Bing, CHU Shoudao, YANG Jing, et al. Main components determination and their contribution to odor in clary sage (Salvia sclarea L.) oil[J]. Journal of Light Industry, 2018, 33(1): 7-12. doi: 10.3969/j.issn.2096-1553.2018.01.002

Citation: BAI Bing, CHU Shoudao, YANG Jing, et al. Main components determination and their contribution to odor in clary sage (Salvia sclarea L.) oil[J]. Journal of Light Industry, 2018, 33(1): 7-12. doi: 10.3969/j.issn.2096-1553.2018.01.002

香紫苏油主成分含量测定及其香气贡献评价

白冰 ¹ ,
楚首道 ¹ ,
杨靖 ¹ ,
张改红 ¹ ,
贾春晓 ² ,
毛多斌 ^{1

,}

郑州轻工业学院食品与生物工程学院, 河南郑州 450001;
郑州轻工业学院材料与化学工程学院, 河南郑州 450001

通讯作者: 毛多斌

基金项目: 中国烟草总公司重点科技项目（110201402033）
中图分类号: TS47

Main components determination and their contribution to odor in clary sage (Salvia sclarea L.) oil

College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China

Corresponding author: MAO Duobin,

Received Date: 2017-04-24
Available Online: 2018-01-15
CLC number: TS47

摘要: 以苯甲醇为内标物，选用HP-5MS毛细管色谱柱，采用GC-MS法对香紫苏油中10种主要成分进行含量测定，并对其香气阈值和香气活力值进行测定和计算.结果表明：该方法可使样品中各组分很好地分离，线性方程相关系数大于0.999，加标回收率为90.6%~97.2%，检出限为9.5~232.8 ng/L，说明该方法重复性和灵敏度良好；香紫苏油中芳樟醇和乙酸芳樟酯的含量分别为20.3%和42.7%，香气阈值较低且香气活力值远高于其他成分，对香紫苏油香气贡献最大，与嗅香评价结果一致.
- 香紫苏油 /
- 气相色谱-质谱联用 /
- 香气阈值 /
- 香气活力值
Abstract: The selected ion monitoring (SIM) was used as the mass spectrum signal acquisition mode. UsingHP-5MS capillary column, a method for determination of ten compounds in clary sage (Salvia sclarea L.) oil has been developed.Odor threshold values of ten compounds were determined,and odor active values were obtained.The results showed that:Benzyl alcohol was used as an internal standard.The samples were separated on a DB-5 capillary column.The selected ion monitoring (SIM) was used as the mass spectrum signal acquisition mode.The components of the sample were well separated in the experimental conditions above,and had good linearity with the correlation coefficients higher than 0.999.The average recoveries were in the range of 90.6%~97.2%.The limits of detection (S/N ≥ 10) were in the range of 9.5~232.8 ng/L, which illustrated the good repetability and sensibility of the method.The compounds in clary sage oil with high odor active value were linaloolwith the content of 20.3% and linalyl acetate with the content of 42.7%.
- clary sage oil /
- gas chromatography-mass spectrometry(GC-MS) /
- odor threshold value /
- odor active value
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白冰, 楚首道, 杨靖, 等. 香紫苏油主成分含量测定及其香气贡献评价[J]. 轻工学报, 2018, 33(1): 7-12. doi: 10.3969/j.issn.2096-1553.2018.01.002

引用本文: 白冰, 楚首道, 杨靖, 等. 香紫苏油主成分含量测定及其香气贡献评价[J]. 轻工学报, 2018, 33(1): 7-12. doi: 10.3969/j.issn.2096-1553.2018.01.002

香紫苏油主成分含量测定及其香气贡献评价

通讯作者: 毛多斌

郑州轻工业学院食品与生物工程学院, 河南郑州 450001;
郑州轻工业学院材料与化学工程学院, 河南郑州 450001

收稿日期: 2017-04-24
网络出版日期: 2018-01-15

基金项目: 中国烟草总公司重点科技项目（110201402033）

关键词:

摘要: 以苯甲醇为内标物，选用HP-5MS毛细管色谱柱，采用GC-MS法对香紫苏油中10种主要成分进行含量测定，并对其香气阈值和香气活力值进行测定和计算.结果表明：该方法可使样品中各组分很好地分离，线性方程相关系数大于0.999，加标回收率为90.6%~97.2%，检出限为9.5~232.8 ng/L，说明该方法重复性和灵敏度良好；香紫苏油中芳樟醇和乙酸芳樟酯的含量分别为20.3%和42.7%，香气阈值较低且香气活力值远高于其他成分，对香紫苏油香气贡献最大，与嗅香评价结果一致.

English Abstract

Main components determination and their contribution to odor in clary sage (Salvia sclarea L.) oil

Corresponding author: MAO Duobin,

College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China

Received Date: 2017-04-24
Available Online: 2018-01-15

Abstract: The selected ion monitoring (SIM) was used as the mass spectrum signal acquisition mode. UsingHP-5MS capillary column, a method for determination of ten compounds in clary sage (Salvia sclarea L.) oil has been developed.Odor threshold values of ten compounds were determined,and odor active values were obtained.The results showed that:Benzyl alcohol was used as an internal standard.The samples were separated on a DB-5 capillary column.The selected ion monitoring (SIM) was used as the mass spectrum signal acquisition mode.The components of the sample were well separated in the experimental conditions above,and had good linearity with the correlation coefficients higher than 0.999.The average recoveries were in the range of 90.6%~97.2%.The limits of detection (S/N ≥ 10) were in the range of 9.5~232.8 ng/L, which illustrated the good repetability and sensibility of the method.The compounds in clary sage oil with high odor active value were linaloolwith the content of 20.3% and linalyl acetate with the content of 42.7%.