中国浓香型白酒“增己降乳”研究与应用进展

何培新; 胡晓龙; 郑燕; 沈祥坤; 李绍亮; 李学思; 范海报

doi:10.3969/j.issn.2096-1553.2018.04.001

中国浓香型白酒“增己降乳”研究与应用进展

郑州轻工业学院食品与生物工程学院, 河南郑州 450001;
河南省食品工业科学研究所有限公司, 河南郑州 450003;
河南宋河酒业股份有限公司, 河南鹿邑 477265;
河南皇沟酒业有限责任公司, 河南永城 476600
基金项目: 2018年度河南省重大科技专项项目（181100211400）
中图分类号: TS261.4

Research and application progress of “Ethyl Caproate-increasing and Ethyl Lactate-decreasing” in brewing of Chinese Luzhou-flavor liquor

College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
He'nan Food Industry Science Research Institute Co., Ltd., Zhengzhou 450003, China;
He'nan Songhe Distillery Co., Ltd., Luyi 477265, China;
He'nan Huanggou Liquor Co., Ltd., Yongcheng 476600, China
Received Date: 2018-06-19
Available Online: 2018-07-15
CLC number: TS261.4

摘要: 适当增加浓香型白酒中己酸乙酯的质量浓度，降低乳酸乙酯的质量浓度，即“增己降乳”，是提高浓香型白酒品质的有效途径.从己酸乙酯和乳酸乙酯的化学性质与生物合成机理、与“增己降乳”相关的微生物及其应用、“增己降乳”综合技术措施等方面对已有文献进行梳理发现：己酸乙酯和乳酸乙酯分别由己酸和乳酸与乙醇通过酯化作用生成，己酸和乳酸分别是这两种酯生物合成的底物；己酸菌、甲烷菌、酯化菌、乳酸菌和酵母菌等都是与“增己降乳”直接或间接相关联的微生物，单独或复合使用这些微生物制剂可以取得明显的“增己降乳”的效果；此外，白酒企业因地、因时制宜，采取科学建造窖池、优化窖泥配方、提高大曲质量、做好清洁卫生、调整入池条件、精细蒸馏操作、低温缓慢发酵等综合措施，也有助于实现“增己降乳”.未来应深入研究不同生产条件下，乳酸菌、降乳菌等“增己降乳”相关微生物的种群演替规律；采用人工培养方法和宏基因组学等免培养技术，系统研究与揭示浓香型白酒酿造过程中各种微生物的生长繁殖和代谢活动规律.以科学发展的态度，规范白酒产业化管理，保持“增己”和“降乳”的合理与适度，从而创造出具有自身特色、适应产品消费结构变化的品牌产品.
- 浓香型白酒 /
- 己酸乙酯 /
- 乳酸乙酯 /
- “增己降乳”
Abstract: Appropriately increasing the mass concentration of ethyl caproate and lowering that of ethyl lactate in Luzhou-flavor liquor, i.e. "Ethyl Caproate-increasing and Ethyl Lactate-decreasing", is an effective way to improve the quality of Luzhou-flavor liquor. From the aspects of the chemical properties and biosynthesis of ethyl caproate and ethyl lactate, the related microorganisms of "Ethyl Caproate-increasing and Ethyl Lactate-decreasing", and the main technical measures of "Ethyl Caproate-increasing and Ethyl Lactate-decreasing", the existing literatures were sorted out.Ethyl hexanoate and ethyl lactate are formed by caproic acid and lactic acid and ethanol through esterification, respectively. Caproic acid and lactic acid are substrates for the biosynthesis of these two esters. Caproic acid, methanogen, esterified bacteria, lactic acid bacteria and yeasts are all directly or indirectly relate to the "Ethyl Caproate-increasing and Ethyl Lactate-decreasing". The application of these microbial preparations alone or in combination can achieve an obvious effect of "Ethyl Caproate-increasing and Ethyl Lactate-decreasing".In addition,in order to achieve the purpose of "Ethyl Caproate-increasing and Ethyl Lactate-decreasing", Luzhou-flavor liquor companies apply some comprehensive measures according to the local conditions and fermented time, such as scientific construction of the wine cellar fermentation tank, optimization of the pit mud, improvement the quality of Daqu, cleaning and hygienism, adjustment of the conditions of fermentation tank, fine distillation operation, and slow fermentation under low temperature, i.e. In the future, we should study the population succession of lactic acid bacteria, lactobacilli, i.e. related to "Ethyl Caproate-increasing and Ethyl Lactate-decreasing" under different production conditions. And use artificial culture method and metagenomics and other culture-free techniques to systematically study and reveal the laws of growth, reproduction and metabolism of various microorganisms through the brewing process of Luzhou-flavor liquor.And the industrialization management of liquor will be regulated with the attitude of scientific development to maintain "Ethyl Caproate-increasing" and "Ethyl Lactate-decreasing" reasonably and appropriately, thus ultimately create their branded products with own characteristics and adapt to changes in product consumption structure.
- Luzhou-flavor liquor /
- ethyl caproate /
- ethyl lactate /
- “Ethyl Caproate-increasing and Ethyl Lactate-decreasing”
1. [1]
  王延才.中国酒业形势[J].酒·饮料技术装备,2016(3):12.
2. [2]
  ZHAO J S,ZHENG J,ZHOU R Q,et al.Microbial community structure of pit mud in a Chinese strong aromatic liquor fermentation pit[J].Journal of the Institute of Brewing,2012,11(4):356.
3. [3]
  中国国家标准委员会.浓香型白酒:GB/T 10781.1-2006[S].北京:中国标准出版社,2006.
4. [4]
  李维青.浓香型白酒与乳酸菌、乳酸、乳酸乙酯[J].酿酒,2010,37(3):90.
5. [5]
  赖登燡,梁诚.系统论对浓香型白酒生产中"增己降乳"的解析及应用[J].酿酒科技,2008(8):104.
6. [6]
  施安辉,刘积和,曲维祺,等.当前浓香型白酒增己降乳的微生物学技术[J].中国酿造,2001(3):8.
7. [7]
  张彬,武绪敬,陈清让.锅底添加酒精串蒸工艺的探讨[J].酿酒科技,2003(2):47.
8. [8]
  谢玉球,钟雨,谢旭,等.乳酸菌在固态法白酒生产中的地位与作用[J].酿酒科技,2008(11):83.
9. [9]
  沈怡方.白酒生产技术全书[M].北京:中国轻工业出版社,1998.
10. [10]
  ZHU X,ZHOU Y,WANG Y,et al.Production of high-concentration n-caproic acid from lactate through fermentation using a newly isolated Ruminococcaceae bacterium CPB6[J].Biotechnology for Biofuels,2017,10:102.
11. [11]
  CAVALCANTE W De A,LEITÃO R C,GEHRING T A,et al.Anaerobic fermentation for n-caproic acid production:a review[J].Process Biochemistry,2017,54:106.
12. [12]
  赵华,赵树欣,李颖宪,等.酵母菌己酸乙酯生物合成与代谢控制育种[J].酿酒科技,1998(4):24.
13. [13]
  刘雪,杨爱华,张学梅,等.华根霉生物酶法合成己酸乙酯条件的研究[J].酿酒科技,2017(2):57.
14. [14]
  詹美榕,丁俊竹,丛方地,等.调节黑曲霉脂肪酶催化合成己酸乙酯的探索[J].酿酒科技,2016(12):28.
15. [15]
  赵华,赵树欣,李颖宪,等.微生物酶法合成己酸乙酯的研究[J].天津轻工业学院学报,1999(1):15.
16. [16]
  周恒刚.漫谈己酸乙酯的酯化[J].酿酒科技,1998(3):18.
17. [17]
  唐瑞.己酸菌、窖泥与浓香型白酒之间的关系[J].酿酒,2005,32(4):24.
18. [18]
  章克昌.酒精与蒸馏酒工艺学[M].北京:中国轻工业出版社,2002.
19. [19]
  何培新.高级微生物学[M].北京:中国轻工业出版社,2017.
20. [20]
  孟东,梁辉.浓香型白酒乳酸乙酯的产生及控制方法[J].江苏食品与发酵,2003(2):21.
21. [21]
  ZHENG J,LIANG R,ZHANG L,et al.Characterization of microbial communities in strong aromatic liquor fermentation pit muds of different ages assessed by combined DGGE and PLFA analyses[J].Food Research International,2013,54(1):660.
22. [22]
  曾田,胡晓龙,马兆,等.浓香型白酒窖泥中"增己降乳"原核微生物群落多样性解析[J].轻工学报,2017,32(6):12.
23. [23]
  李洪祥.己酸菌培养液使用小议[J].酿酒科技,2002(5):40.
24. [24]
  杨喜军.黑轻80号己酸菌在我厂白酒生产中的应用[J].黑龙江发酵,1982(1):31.
25. [25]
  施安辉.在白酒酿造中己酸菌C1应用方法的对比试验[J].中国酿造,1983,2(6):25.
26. [26]
  管健,廖蓓,李兆飞,等.白酒功能菌的功能特性及应用研究[J].中国酿造,2017,36(8):1.
27. [27]
  彭文明,周敬慧,刘娟.己酸菌复合菌液在浓香型白酒生产中的应用[J].中小企业管理与科技(中旬刊),2014(20):311.
28. [28]
  黄丹,刘超兰,张文学,等.白酒发酵窖泥中一株产氢细菌的分离及其代谢特性分析[J].酿酒科技,2013(12):21.
29. [29]
  邓杰,卫春会,边名鸿,等.浓香型白酒不同窖龄窖池窖泥中古菌群落结构分析[J].食品科学,2017,38(8):37.
30. [30]
  颜昌轩.甲烷菌在浓香型白酒生产中的作用(简报)[J].西南大学学报(自然科学版),1989,11(5):481.
31. [31]
  梁龙元,王露,薛栋升.白酒酿造中酯酶及己酸乙酯的研究进展[J].酿酒,2018,45(1):17.
32. [32]
  任道群,唐玉明,姚万春,等.酯化酶动力学研究[J].酿酒科技,2006(6):39.
33. [33]
  ARANDA J,CERQUEIRA N M F S A,FERNANDES P A,et al.The catalytic mechanism of carboxylesterases:a computational study[J].Biochemistry,2014,53(36):5820.
34. [34]
  施安辉,张文璞,徐恩润,等.酯酶的微生物类群、酯化特性及应用前景[J].山东食品发酵,2001(2):17.
35. [35]
  SMART K.Brewing yeast fermentation performance[M].Oxford:Blackwell Science Ltd,A Blackwell Publishing Company,2003.
36. [36]
  王治国,夏明星,管清先,等.应用产酯酵母提高浓香型白酒质量的研究[J].酿酒科技,1994(4):13.
37. [37]
  侯小歌,王俊英,李学思,等.浓香型白酒糟醅及窖泥产香功能菌的研究进展[J].微生物学通报,2013,40(7):1257.
38. [38]
  胡晓龙,赵东,葛隐,等.五粮液大曲中产酯化酶红曲霉的分离及酯化酶的纯化[J].酿酒,2011,38(3):35.
39. [39]
  黄丹,储玉龙,尚志超,等.大曲酯化酶根霉菌的分离及产酶条件研究[J].食品与发酵科技,2010,46(3):30.
40. [40]
  滕巍,李国莹,刘小波,等.大曲中产酯化酶菌株的分离鉴定及固体发酵工艺优化[J].食品与生物技术学报,2016,35(9):971.
41. [41]
  李绍亮,李学思,侯小歌,等.宋河酒曲中主要霉菌的鉴定及其产酶特性的研究[J].酿酒,2016,43(6):24.
42. [42]
  镇达,尚玲,陈茂彬.红曲霉酯化酶特性及在白酒酿造中的应用研究[J].酿酒科技,2009(1):62.
43. [43]
  黄丹,张强,严芳,等.浓香型大曲中酯化酶细菌的分离鉴定及产酶条件研究[J].中国酿造,2009(8):57.
44. [44]
  张秀红,刘秋林,孔健,等.一株产酯化酶细菌的筛选[J].酿酒科技,2012(6):56.
45. [45]
  刘阳.中高温大曲中产酯化酶细菌的选育[D].芜湖:安徽工程大学,2013.
46. [46]
  朱弟雄,涂向勇.酯化红曲在浓香型白酒生产度夏压排中的应用研究[J].酿酒科技,2010(5):82.
47. [47]
  刘义刚,周治全,周超,等.全细胞酯化曲(酶)在生物酯化液中的应用[J].酿酒科技,2016(2):72.
48. [48]
  姚惟琦,陈茂彬,镇达,等.浓香型白酒酒醅中乳酸菌分离及其对模拟固态发酵的影响[J].酿酒,2010,37(3):37.
49. [49]
  栗连会.泸型酒酒醅中乳酸菌和乳酸降解菌的多样性和代谢特性[D].无锡:江南大学,2016.
50. [50]
  孙超,刘勇.白酒生产中乳酸菌的分布及主要代谢产物[J].中国酿造,2012,31(5):1.
51. [51]
  李大和.浓香型曲酒乳酸乙酯偏高的原因及解决措施[J].酿酒科技,2007(2):100.
52. [52]
  郭本跃.生香ADY,TH-AADY,己酸菌液和糖化酶混合使用提高浓香型曲酒的质量[J].酿酒科技,2002(5):87.
53. [53]
  徐希望,徐世江,谢恩举.利用丙、己酸菌液双喷窖实现增己降乳的研究[J].酿酒科技,1996(6):43.
54. [54]
  张志刚,周爱江.复合酯化酶生态菌剂在浓香型白酒中的应用研究[J].酿酒科技,2006(5):55.
55. [55]
  赖登燡.浓香型白酒生产中"增己降乳"科学、合理性的研究[J].酿酒,2007,34(5):4.
56. [56]
  詹炳耀.浓香型大曲酒"增己抑乳"问题的探讨与研究[J].黑龙江发酵,1982(3):34.
57. [57]
  戴自鸣,汪俊英."增己减乳"的一项技术措施(摘要)[J].酿酒,1982(4):38.
58. [58]
  詹炳耀,郭宗武.低度张弓大曲酒[J].黑龙江发酵,1981(4):12.
59. [59]
  冯雅芳,张永利.凤型酒生产过程降乳措施探讨[J].酿酒,2015,42(6):89.
60. [60]
  田以清,牛学士.浅谈增己降乳提高浓香型白酒质量[J].酿酒科技,1992(6):22.
61. [61]
  钟玉叶,崔如生.洋河酒厂的多粮工艺创新[J].酿酒,2007,34(5):28.
62. [62]
  杨平,涂荣坤,钱志伟,等.甑桶醅层高度及结构设计对蒸馏效率及酒质的影响研究[J].酿酒科技,2012(10):94.
63. [63]
  陈全庚,陈珊,黄应华,等.气封甑和水封甑蒸馏对比试验研究[J].酿酒科技,2012(4):88.
64. [64]
  陈全庚,孙庆文,黄应华,等.应用白酒气态处理新技术提高蒸馏提香效果和原酒品质的生产实验[J].酿酒科技,2013(1):65.
65. [65]
  孙庆文,陈全庚,侯勇,等.气封甑蒸馏效果分析研究[J].酿酒科技,2014(4):57.
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沈阳化工大学材料科学与工程学院沈阳 110142

中国浓香型白酒“增己降乳”研究与应用进展

Research and application progress of “Ethyl Caproate-increasing and Ethyl Lactate-decreasing” in brewing of Chinese Luzhou-flavor liquor

计量

通讯作者: 陈斌, bchen63@163.com

中国浓香型白酒“增己降乳”研究与应用进展

English Abstract

Research and application progress of “Ethyl Caproate-increasing and Ethyl Lactate-decreasing” in brewing of Chinese Luzhou-flavor liquor

目录

中国浓香型白酒“增己降乳”研究与应用进展

Research and application progress of “Ethyl Caproate-increasing and Ethyl Lactate-decreasing” in brewing of Chinese Luzhou-flavor liquor

计量

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通讯作者: 陈斌, bchen63@163.com

中国浓香型白酒“增己降乳”研究与应用进展

English Abstract

Research and application progress of “Ethyl Caproate-increasing and Ethyl Lactate-decreasing” in brewing of Chinese Luzhou-flavor liquor

目录