款冬叶黄酮的抗氧化活性研究
Research on the antioxidant activity of coltsfoot leaf flavonoid
-
摘要: 采用超声波辅助提取款冬叶黄酮,利用吸附树脂对其进行纯化,进而探讨款冬叶黄酮体外抗氧化活性(DPPH+·清除力和Fe3+还原力)及其对亚急性衰老模型小鼠体内抗氧化活性(T-AOC,SOD活性,GSH-PX活性和MDA含量)的影响.结果表明:款冬叶黄酮具有良好的抗氧化活性,可提高DPPH+·清除力和Fe3+还原力,并可显著提高亚急性衰老模型小鼠体内的T-AOC,SOD活性和GSH-PX活性,降低MDA含量.款冬叶黄酮良好的抗氧化活性可应用于抗衰老产品的研究,为我国中草药活性成分的提取与应用提供参考.Abstract: Using ultrasonic auxiliary to extract coltsfoot leaf flavonoid,and to purify it by adsorption resin,coltsfoot leaf flavonoid in vitro antioxidant activity (DPPH+·scavenging force and Fe3+ reducing force) and its influence on antioxidant activity of subacute aging model mice (T-AOC,SOD activity,GSH-PX activity and MDA content) were explored.The results showed that the coltsfoot leaf flavonoid had good antioxidant activity,and they could improve the Fe3+ reducing force and DPPH+·scavenging force,and obviously improve the T-AOC,SOD and GSH-PX activity of the subacute aging model mice and reduce MDA content.The good antioxidant activity of coltsfoot leaf flavonoid could be applied to promote anti-aging research and provide a reference for the extraction and application of active ingredient in Chinese herbal medicine.
-
Key words:
- coltsfoot leaf /
- flavonoid /
- aging model mice /
- antioxidant activity
-
-
[1]
刘毅,王允,万德光,等.款冬花本草考证[J].中药材,2010,33(4):634.
-
[2]
刘佳,孙国强,秦梦,等.款冬花的本草考证[J].中国现代应用药学,2018,35(2):204.
-
[3]
刘玉峰,杨秀伟,武滨.款冬花化学成分的研究[J].中国中药杂志,2007,32(22):2378.
-
[4]
朱丹,袁芳,孟坤,等.黄酮类化合物的研究进展[J].中华中医药杂志,2007,22(6):387.
-
[5]
梁树才,宗自卫,于海英,等.荷叶总黄酮对小鼠酒精肝损伤的保护作用[J].食品工业科技,2014,35(9):347.
-
[6]
任虹,张乃元,田文静,等.源于果蔬的黄酮类化合物及其抗肿瘤作用靶点研究进展[J].食品科学,2013,34(11):321.
-
[7]
曾佑炜.黄酮抗癌作用研究进展[J].天然产物研究与开发,2016(11):1838.
-
[8]
卢秋玉,陈晓宇,申庆荣,等.木棉花总黄酮降血脂作用及其机制研究[J].中药药理与临床,2016(1):88.
-
[9]
李淑珍,李进.黑果枸杞总黄酮降血脂作用[J].时珍国医国药,2012,23(5):1072.
-
[10]
李鹏程,朴春红,张岚,等.荞麦壳黄酮提取物对2型糖尿病大鼠的血糖改善作用及机制[J].食品科学,2017,38(5):244.
-
[11]
宋逍, 段玺, 唐志书,等. 微波辅助提取款冬花多糖的工艺及抗氧化活性研究[J].中国医学创新, 2016(23):27.
-
[12]
程晓叶,张霞,廖曼,等.UPLC-Q-TOF-MS法分析款冬花的化学成分[J].中草药,2017(12):2390.
-
[13]
乔月,陈丽华,胡宏秀,等.基于UPLC-MS/MS同时测定款冬花中吡咯里西啶生物碱及其氮氧化物的含量[J].中国实验方剂学杂志, 2016(1):155.
-
[14]
丁彩丽,吴鸣建,程向红,等.SP825大孔吸附树脂纯化款冬花中总黄酮的研究[J].食品科技,2009,34(6):220.
-
[15]
CENH Z,BERTIN R,FROLDI G.EC50 estimation of antioxidant activity in DPPH·assay using several statistical programs[J].Food Chemistry,2013,138(1):414.
-
[16]
MA Z,HUANG T,LI Y,et al.Study on antioxidant activity in vitro of methanol extract of jasminum sambac leaves[J].Chinese Wild Plant Resources,2015,34(3):20.
-
[17]
丁豪,杨海燕,辛志宏.昆仑雪菊黄酮类化合物的抗氧化相互作用研究[J].食品科学,2015,21:26.
-
[1]
计量
- PDF下载量: 9
- 文章访问数: 1776
- 引证文献数: 0