DU Shaoping, HU Haiyan, GAN Xiangwu, et al. Study on high-density fermentation of β-mannanase produced by constitutive Pichia pastoris[J]. Journal of Light Industry, 2020, 35(4): 1-7. doi: 10.12187/2020.04.001
Citation:
DU Shaoping, HU Haiyan, GAN Xiangwu, et al. Study on high-density fermentation of β-mannanase produced by constitutive Pichia pastoris[J]. Journal of Light Industry, 2020, 35(4): 1-7.
doi:
10.12187/2020.04.001
Study on high-density fermentation of β-mannanase produced by constitutive Pichia pastoris
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Received Date:
2020-05-21
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Abstract
The carbon and nitrogen sources of the constitutive Pichia pastoris X-33/pGAPZαA-Man26A-39 fermentation medium were optimized by shake flask experiment,and the fermentation conditions were regulated in a 50 L fermentor by using intermittent feeding strategy,in order to realize high-density fermentation of constitutive Pichia pastoris to produce β-mannanase.The results showed that glucose was the most suitable carbon source for fermentation medium,and its initial centration was 30 g/L;corn syrup was the most suitable nitrogen source,and its initial mass fraction was 6%;during the regulation of intermittent fed fermentation,the pH value of bacteria could be maintained between 5.0~5.5 by intermittent flow adding 25% ammonia hydroxide,50% glucose solution and 20% corn syrup,and make sure the carbon and nitrogen sources of the fermentation medium were sufficient;after 60 h fermentation,took measures to put the fermentater tank,at this time,the maximum enzyme activity was 2 685.5 U/mL,the mass concentration of bacteria reached 331.1 g/L,and the OD600 reached 302.8,which achieved the high expression of β-mannanase.
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Proportional views
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