JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 35 Issue 4
July 2020
Article Contents
DU Shaoping, HU Haiyan, GAN Xiangwu, et al. Study on high-density fermentation of β-mannanase produced by constitutive Pichia pastoris[J]. Journal of Light Industry, 2020, 35(4): 1-7. doi: 10.12187/2020.04.001
Citation: DU Shaoping, HU Haiyan, GAN Xiangwu, et al. Study on high-density fermentation of β-mannanase produced by constitutive Pichia pastoris[J]. Journal of Light Industry, 2020, 35(4): 1-7. doi: 10.12187/2020.04.001 shu

Study on high-density fermentation of β-mannanase produced by constitutive Pichia pastoris

  • Received Date: 2020-05-21
  • The carbon and nitrogen sources of the constitutive Pichia pastoris X-33/pGAPZαA-Man26A-39 fermentation medium were optimized by shake flask experiment,and the fermentation conditions were regulated in a 50 L fermentor by using intermittent feeding strategy,in order to realize high-density fermentation of constitutive Pichia pastoris to produce β-mannanase.The results showed that glucose was the most suitable carbon source for fermentation medium,and its initial centration was 30 g/L;corn syrup was the most suitable nitrogen source,and its initial mass fraction was 6%;during the regulation of intermittent fed fermentation,the pH value of bacteria could be maintained between 5.0~5.5 by intermittent flow adding 25% ammonia hydroxide,50% glucose solution and 20% corn syrup,and make sure the carbon and nitrogen sources of the fermentation medium were sufficient;after 60 h fermentation,took measures to put the fermentater tank,at this time,the maximum enzyme activity was 2 685.5 U/mL,the mass concentration of bacteria reached 331.1 g/L,and the OD600 reached 302.8,which achieved the high expression of β-mannanase.
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    1. [1]

      SCHELLER H V,ULVSKOV P.Hemicelluloses[J].Annual Review of Plant Biology,2010,61:263.

    2. [2]

      STÅLBRAND H,SIIKAAHO M,TENKANEN M,et al.Purification and characterization of two β-mannanases from Trichoderma reesei[J].Journal of Biotechnology,1993,29(3):229.

    3. [3]

      KANSOH A L,NAGIEB Z A.Xylanase and mannanase enzymes from Streptomyces galbus NR and their use in biobleaching of softwood kraft pulp[J].Antonie Van Leeuwonhoek,2004,85(2):103.

    4. [4]

      NUNES C S,MALMLÖF K.Effects of guar gum and cellulose on glucose absorption,hormonal release and hepatic metabolism in the pig[J].British Journal of Nutrition,1992,68(3):693.

    5. [5]

      BHAT M K.Cellulases and related enzymes in biotechnology[J].Biotechnology Advances,2000,18(5):355.

    6. [6]

      熊进,黄魁英,夏枫耿,等.耐高温β-甘露聚糖酶M27-8菌株发酵条件的优化[J].中国饲料,2017,19:13.

    7. [7]

      吴秀秀,吕晓慧,胡亚冬,等.耐高温耐酸稳定假密环菌(Armillariella tabescens)MAN47β-甘露聚糖酶体外分子定向进化[J].中国生物工程杂志,2012,32(3):83.

    8. [8]

      ZHAO W,ZHENG J,ZHOU H B,et al.A thermotolerant and cold-active mannan endo-1,4-β-mannosidase from Aspergillus niger CBS 513.88:Constitutive overexpression and high-density fermentation in Pichia pastoris[J].Bioresource Technology,2011,102:7538.

    9. [9]

      ZHENG J,ZHAO W,GUO N,et al.Development of an industrial medium and a novel fed-batch strategyfor high-level expression of recombinant β-mananase by Pichia pastoris[J].Bioresource Technology,2012,118:257.

    10. [10]

      赵凯,许鹏举,谷光烨.3,5-二硝基水杨酸比色法测定还原糖含量的研究[J].食品科学,2008,29(8):534.

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