带鱼蛋白酶解工艺条件的响应面优化
Response surface optimization of proteolysis technological conditions of hairtail
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摘要: 以带鱼为原材料，从6种蛋白酶中筛选出碱性蛋白酶作为带鱼蛋白酶解的最佳蛋白酶，进而利用单因素试验和响应面试验优化带鱼蛋白酶解的工艺条件.结果表明：带鱼蛋白酶解的最佳工艺条件为底物质量分数5.3%，蛋白酶质量分数2.7%，酶解pH值8.3，酶解温度63℃，酶解时间168 min，在该工艺条件下，带鱼蛋白水解度达到37.07%，与响应面优化试验回归模型的预测值基本一致.Abstract: Taking hairtail as raw material,alkaline protease was selected from 6 kinds of proteases as the best protease for hairtail proteolysis,and the technological conditions of hairtail proteolysis were optimized by single factor test and response surface test.The results showed that the optimum conditions for proteolysis of hairtail were as follows:the substrate mass fraction was 5.3%,the protease mass fraction was 2.7%,the pH value of hydrolysis was 8.3,the hydrolysis temperature was 63℃,and the hydrolysis time was 168 min.Under these conditions,the hydrolysis degree of hairtail protein reached 37.07%,which was basically consistent with the predicted value of regression model of response surface optimization test.
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Key words:
- hairtail /
- proteolysis /
- alkaline protease /
- response surface optimization
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