JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 36 Issue 6
December 2021
Article Contents
YAN Hongyang, HUANG Qimeng, CAI Xinghua, et al. Isolation and identification of aroma-producing strains and application of fermentation products in cigarette flavoring[J]. Journal of Light Industry, 2021, 36(6): 47-54. doi: 10.12187/2021.06.006
Citation: YAN Hongyang, HUANG Qimeng, CAI Xinghua, et al. Isolation and identification of aroma-producing strains and application of fermentation products in cigarette flavoring[J]. Journal of Light Industry, 2021, 36(6): 47-54. doi: 10.12187/2021.06.006 shu

Isolation and identification of aroma-producing strains and application of fermentation products in cigarette flavoring

  • Received Date: 2021-01-31
  • An aroma-producing strain MG6 with aroma production was isolated from the surface of sunny rose grape. The strain was identified and the characteristic aroma components of its fermentation broth were analyzed. Then the tobacco flavor was prepared by the strain and the aroma evaluation of cigarette was carried out. The results showed that the strain was Pichia terricola. Compared with the control group, the main characteristic aroma components such as ketones, alcohols, esters, phenols and olefins in the fermentation broth of the strain were significantly increased, and the total contents of megastigmatrien-3-one, dihydrokiwifruit lactone and neophytadiene were increased in different degrees, which were particularly important for improving the aroma quality of tobacco. When the added amount of tobacco flavoring was 0. 05%, the quality and amount of aroma were improved, the irritation was reduced, and the overall sensory quality was improved.
  • 加载中
    1. [1]

      高海有,刘秀明,高莉,等. 烟用香精香料研究现状与发展趋势[J]. 香料香精化妆品,2019(2):70.

    2. [2]

      喻阑清. 微生物发酵烟用香料液研究[D]. 贵阳:贵州大学,2018.

    3. [3]

      薛云,白家峰,严俊,等. 烟用天然香料的研究进展[J]. 轻工科技,2015,31(10):11.

    4. [4]

      张峰,操晓亮,伊勇涛,等. 茶树花纯露的制备及其在卷烟中的应用[J]. 中国烟草学报, 2020,26(5):18.

    5. [5]

      MALDONADO-ROBLEDO G, RODRIGUEZ-BUSTAMANTE E,SANCHEZ-CONTRERAS A,et al. Production of tobacco aroma from lutein. Specific role of the microorganisms involved in the process[J]. Applied Microbiology and Biotechnology, 2003,62(5/6):484.

    6. [6]

      高锐,杨威,宋鹏飞, 等. 微生物制备烟用香料的研究进展[J]. 安徽农业科学,2017,45(2):92.

    7. [7]

      ETSCHMANN M M W, SELL D, SCHRADER J. Medium optimization for the production of the aroma compound 2-phenylethanol using a genetic algorithm[J]. Journal of Molecular Catalysis B Enzymatic,2004,29(6):187.

    8. [8]

      LIU H G, HE H L, CHENG C H, et al. Diversity analysis of the bacterial community in tobacco waste extract during reconstituted tobacco process[J]. Applied Microbiology and Biotechnology,2015,99(1):469.

    9. [9]

      吕品,李丹,黄龙,等. 烟叶产香菌的分离及其在烟用香料制备中的应用[J]. 烟草科技, 2009(1):37.

    10. [10]

      庹有朋,王刚,段旺军,等. 利用生物技术对烤烟发酵的研究[J]. 食品与发酵科技,2020,56(1):69.

    11. [11]

      周丽娟,郑琳,刘煜宇,等. 荔枝产香内生菌的挥发性成分分析及其在卷烟中的应用研究[J]. 精细化工,2010,27(10):1013.

    12. [12]

      朱宇,郑琳,刘煜宇,等. 西瓜内生菌的挥发性成分分析及其发酵香料在卷烟中的应用[J]. 光谱实验室,2011,28(4):2093.

    13. [13]

      郭林青,朴永革,朱春阳,等. 烟草产香酵母YG-4的筛选鉴定及香气成分分析[J]. 轻工学报,2019,34(5):27.

    14. [14]

      张俊杰,尚益民,程大伟,等. 河南安阳赤霞珠葡萄果表酵母菌的分离与鉴定[J]. 轻工学报,2018,33(3):39.

    15. [15]

      温雅骄. 干红葡萄酒酿酒酵母的优选与鉴定及其酿酒品质评价[D]. 呼和浩特:内蒙古农业大学,2019.

    16. [16]

      张俊杰,杨旭,郭晨,等. 不同葡萄品种栽培土壤中酵母菌的组成及差异分析[J]. 酿酒, 2017,44(1):76.

    17. [17]

      管庆林,周笑犁,赵姗,等. 番茄自然发酵液中酵母菌的分离鉴定及其特性研究[J]. 食品工业科技,2021,42(3):96.

    18. [18]

      陈丽花,任丽霞,李东娜,等. 甜酒曲中优质酵母菌的分离鉴定及其产香特性分析[J]. 食品科学,2021,42(6):142.

    19. [19]

      张俊杰,尚益民,彭姗姗,等. 产香酵母的筛选及其苹果酒发酵特性[J]. 中国酿造,2019,38(8):31.

    20. [20]

      MONTROCHER R, VERNER M C, BRIOLAY J, et al. Phylogenetic analysis of the Saccharomyces cerevisiae group based on polymorphisms of rDNA spacer sequences[J]. International Journal of Systematic Bacteriology, 1998, 48(1):295.

    21. [21]

      FELL J W, BOEKHOUT T, FONSECA A, et al. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis[J]. International Journal of Systematic and Evolutionary Microbiology,2000,50(3):1351.

    22. [22]

      FERNÁDEZ-ESPINAR M T,ESTEVE-ZARZOSO B,QUEROL A,et al. RFLP analysis of the ribosomal internal transcribed spacers and the 5.8S rRNA gene region of the genus Saccharomyces:a fast method for species identification and the differentiation of flor yeasts[J]. Antonie Van Leeuwenhoek,2000,78(1):87.

    23. [23]

      国家烟草专卖局. 烟草在制品感官评价方法:YC/T 415-2011[S]. 北京:中国标准出版社,2011.

    24. [24]

      卿蔓君,白梅,张勇,等. 西藏曲拉和云南乳饼中酵母菌的鉴定及其生物多样性[J]. 微生物学报,2010,50(9):1141.

    25. [25]

      LORLIAM W, AKARACHARANYA A, JINDAMORAKOT S, et al. Characterization of xylose-utilizing yeasts isolated from herbivore faeces in Thailand[J]. Scienceasia, 2013, 39(1):26.

    26. [26]

      KURTZMAN C P,ROBNETT C J,BASEHOARPOWERS E. Phylodenetic relationship among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyes gen. nov.[J]. FEMS Yeast Research,2008(6):939.

    27. [27]

      陈思睿,唐琳琳,冯建文,等. 高效降解柠檬酸酵母菌的筛选鉴定及其在红树莓果汁中降酸特性[J]. 食品科学,2020,635(22):142.

Article Metrics

Article views(3365) PDF downloads(56) Cited by()

Ralated
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return