JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

柚子幼果精油的提取及其抗氧化活性研究

庄姗姗 ,  刘袆帆 ,  张沁铭 ,  肖更生 ,  戴宏杰 ,  马路凯

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庄姗姗, 刘袆帆, 张沁铭, 等. 柚子幼果精油的提取及其抗氧化活性研究[J]. 轻工学报, 2022, 37(2): 51-58. doi: 10.12187/2022.02.007
引用本文: 庄姗姗, 刘袆帆, 张沁铭, 等. 柚子幼果精油的提取及其抗氧化活性研究[J]. 轻工学报, 2022, 37(2): 51-58. doi: 10.12187/2022.02.007
shu
ZHUANG Shanshan, LIU Huifan, ZHANG Qinming, et al. Study on extraction and antioxidant activity of essential oil from grapefruit fruitlet[J]. Journal of Light Industry, 2022, 37(2): 51-58. doi: 10.12187/2022.02.007
Citation: ZHUANG Shanshan, LIU Huifan, ZHANG Qinming, et al. Study on extraction and antioxidant activity of essential oil from grapefruit fruitlet[J]. Journal of Light Industry, 2022, 37(2): 51-58. doi: 10.12187/2022.02.007
shu

柚子幼果精油的提取及其抗氧化活性研究

  • 1. 仲恺农业工程学院 轻工食品学院/广东省岭南特色食品科学与技术重点实验室, 广东 广州 510225;

  • 2. 广东省梅州市梅县区农业科学研究所, 广东 梅州 514783;

  • 3. 西南大学 食品科学学院, 重庆 400715;

  • 4. 仲恺农业工程学院 现代农业创新研究院, 广东 广州 510225

    • 作者简介: 庄姗姗(1995—),女,广东省揭阳市人,仲恺农业工程学院硕士研究生,主要研究方向为食品加工与安全。E-mail:987045601@qq.com;

  • 基金项目: 国家自然科学基金项目(32001622)
    广东省岭南特色食品科学与技术重点实验室项目(2021B1212040013)
    国家重点研发计划项目(2019YFD1002300)

  • 中图分类号: TS225.1

Study on extraction and antioxidant activity of essential oil from grapefruit fruitlet

  • 1. Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology/College of Light Industry and Food Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China;

  • 2. Agricultural Science Research Institute of Meixian District, Meizhou 514783, China;

  • 3. College of Food Science, Southwest University, Chongqing 400715, China;

  • 4. Academy of Contemporary Agricultural Engineering Innovations, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China

  • Received Date: 2021-06-21
    Accepted Date: 2022-01-19

    CLC number: TS225.1

  • 摘要: 以柚子幼果为原料,采用亚临界流体萃取技术提取其精油,利用GC-MS联用技术对精油的化学成分进行分析,并应用AAPH诱导红细胞氧化应激模型对精油的抗氧化活性进行初步研究。结果表明:柚子幼果精油中共鉴定出126种有效成分,主要包括醇类、萜烯类、酮类、酯类、醛类、酚类和烷烃类7类化合物,其中醇类为主要化合物,共11种,相对含量为16.43%,萜烯类共10种,相对含量为5.37%,酮类共10种,相对含量为3.71%;经柚子幼果精油预处理后,AAPH诱导的红细胞溶血受到抑制,细胞外的MDA浓度和LDH释放量均减少,在保护细胞膜完整性的同时,也缓解了细胞的氧化应激反应,即柚子幼果精油能够通过清除自由基抑制AAPH引起的红细胞溶血。
    Abstract: Subcritical fluid extraction was used to extract the essential oil from grapefruit fruitlet, The chemical composition of the essential oil was analyzed by GC-MS, the antioxidant activity of the essential oil was preliminally investigated by using AAPH induced hemolysis model. The results showed that a total of 126 active components were identified in the essential oil of grapefruit fruitlet, including 7 main compounds: alcohols, terpenes, ketones, esters, aldehydes, phenols and alkanes. Alcohols were the main compounds, with a total of 11 species and a relative content of 16.43%. There were 10 terpenes with a relative content of 5.37%, and 10 ketones, with a relative content of 3.71%. After grapefruit fruitlet essential oil pretreatment, the erythrocyte hemolysis induced by AAPH was restrained, MDA concentration and LDH release quantity in extracellular decreased, which not only protected the cell membrane integrity, but also reduced oxidative stress reaction in the cell. It could be concluded that grapefruit fruitlet essential oil was able to remove free radicals, thereby inhibiting AAPH cause erythrocyte hemolysis.
    1. [1]

      王琴,罗洁莹,柳建良,等.响应面法优化超声-微波协同辅助提取金柚幼果总黄酮工艺[J].食品研究与开发,2020,41(2):83-91.

    2. [2]

      FURUKAWA Y,HARA R I,NAKAYA M,et al.Citrus auraptene induces glial cell line-derived neurotrophic factor in C6 cells[J].International Journal of Molecular Sciences,2020,21(1):253.

    3. [3]

      李宁,杨小红.蛇床子有效成分对金黄色葡萄球菌凝固酶活性的影响[J].广东医学,2007(6):876-877.

    4. [4]

      沈秀,周则卫,刘培勋,等.蛇床子素对荷瘤小鼠的抗肿瘤作用[J].中华医学实践杂志,2006,5(3):241-243.

    5. [5]

      FURUKAWA Y,WASHIMI Y S,HARA R I,et al.Citrus auraptene induces expression of brain-derived neurotrophic factor in Neuro2a cells[J].Molecules,2020,25(5):1117.

    6. [6]

      宋丽丽,李科娜,杨旭,等.小黄姜精油提取工艺优化、成分分析及其生物活性研究[J].轻工学报,2020,35(5):7-15.

    7. [7]

      李嫒.海棠果实提取物抗氧化活性及对胃癌细胞BGC-803的体外抑制活性研究[D].咸阳:西北农林科技大学,2018.

    8. [8]

      李乐,庄斐尔,赵更生,等.蛇床子素对麻醉开胸犬心电图和血流动力学的影响[J].中国药理学与毒理学杂志,1994(2):119-121.

    9. [9]

      李泮霖,李锋,余剑军,等.化州柚幼果生长过程中柚皮苷含量的动态变化研究[J].中药材,2012,35(4):538-539.

    10. [10]

      林家逊,许有瑞,顾生玖,等.GC-MS法分析沙田柚幼果中挥发油化学成分的研究[J].安徽农业科学,2008(24):10527-10537.

    11. [11]

      姜红宇,刘郁峰,盘雪,等.响应面法优化胡柚幼果中柚皮苷与新橙皮苷的提取工艺[J].中国现代应用药学,2017,34(11):1546-1551.

    12. [12]

      许有瑞,聂琼嵘,顾生玖,等.沙田柚幼果中柚皮苷提取工艺研究[J].安徽农业科学,2008(18):7525-7534.

    13. [13]

      ZHANG M M,ZHANG H,LI H X,et al.Antioxidant mechanism of betaine without free radicals scavenging ability[J].Journal of Agricultural & Food Chemistry,2016,64(42):7921-7930.

    14. [14]

      WANG Q,LUO J Y,LIU H F,et al.Protective effects of the flavonoid fraction obtained from pomelo fruitlets through ultrasonic-associated microwave extraction against AAPH-induced erythrocyte hemolysis[J].RSC Advances,2019,9(28):16007-16017.

    15. [15]

      LIU J,CHEN P,HE J,et al.Extraction of oil from Jatropha curcas seeds by subcritical fluid extraction[J].Industrial Crops and Products,2014,62:235-241.

    16. [16]

      丘苑新,何娣,马路凯,等.亚临界丁烷萃取柚籽油的工艺研究及其营养评价[J].粮食与油脂,2021,34(6):78-81.

    17. [17]

      康林芝,王娜,云帆,等.GC-MS法测定不同温度处理的莲子心精油成分含量变化[J].农产品加工,2015(10):41-42,47.

    18. [18]

      WANG W Y,SHI H,ZHU J H,et al.Purification and structural characterization of a novel antioxidant and antibacterial protein from Arca inflata[J].International Journal of Biological Macromolecules,2018,116:289-298.

    19. [19]

      BURITS M,BUCAR F.Antioxidant activity of Nigella sativa essential oil[J].Phytotherapy Research,2015,14(5):323-328.

    20. [20]

      DE MORAES J,DE OLIVEIRA R N,COSTA J P,et al.Phytol, a diterpene alcohol from chlorophyll, as a drug against neglected tropical disease Schistosomiasis mansoni[J].PLoS Negl Trop Dis,2014,8(1):e2617.

    21. [21]

      XU X L,ZHU Q,LV(LYU) H,et al.Isolation and biological activities of citral from sweet orange oil[J].Advanced Materials Research,2013(750/751/752):1621-1625.

    22. [22]

      HUI W,XI H R,LAI F R,et al.Chemical and cellular antioxidant activity of flavone extracts of Labisia pumila before and after in vitro gastrointestinal digestion[J].RSC Advances,2018,8(22):12116-12126.

    23. [23]

      HEMPEL N,MELENDEZ J A.Intracellular redox status controls membrane localization of pro-and anti-migratory signaling molecules[J].Redox Biology,2014,2:245-250.

    24. [24]

      WU J H,HUO J Y,HUANG M Q,et al.Structural characterization of a tetrapeptide from Sesame flavor-type Baijiu and its preventive effects against AAPH-induced oxidative stress in HepG2 cells[J].Journal of Agricultural & Food Chemistry,2017,65(48):10495-10504.

    25. [25]

      DU Y C,ESFANDI R,WILLIAM W G,et al.Antioxidant activity of oat proteins derived peptides in stressed hepatic HepG2 cells[J].Antioxidants,2016,5(4):39.

    26. [26]

      ZHANG M M,ZHANG H,LI H X,LAI F,et al.Antioxidant mechanism of betaine without free radicals scavenging ability[J].Journal of Agricultural and Food Chemistry,2016,64(42):7921-7930.

    27. [27]

      GIRIDHARAN V V,THANDAVARAYAN R A,KONISHI T.Amelioration of scopolamine induced cognitive dysfunction and oxidative stress by inonotus obliquus:A medicinal mushroom[J].Food & Function,2011,2(6):320-327.

    28. [28]

      龙娅,胡文忠,李元政,等.植物精油的抗氧化活性及其在果蔬保鲜上的应用研究进展[J].食品工业科技,2019,40(23):343-348.

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庄姗姗, 刘袆帆, 张沁铭, 等. 柚子幼果精油的提取及其抗氧化活性研究[J]. 轻工学报, 2022, 37(2): 51-58. doi: 10.12187/2022.02.007
引用本文: 庄姗姗, 刘袆帆, 张沁铭, 等. 柚子幼果精油的提取及其抗氧化活性研究[J]. 轻工学报, 2022, 37(2): 51-58. doi: 10.12187/2022.02.007
shu
ZHUANG Shanshan, LIU Huifan, ZHANG Qinming, et al. Study on extraction and antioxidant activity of essential oil from grapefruit fruitlet[J]. Journal of Light Industry, 2022, 37(2): 51-58. doi: 10.12187/2022.02.007
Citation: ZHUANG Shanshan, LIU Huifan, ZHANG Qinming, et al. Study on extraction and antioxidant activity of essential oil from grapefruit fruitlet[J]. Journal of Light Industry, 2022, 37(2): 51-58. doi: 10.12187/2022.02.007
shu

柚子幼果精油的提取及其抗氧化活性研究

    作者简介:庄姗姗(1995—),女,广东省揭阳市人,仲恺农业工程学院硕士研究生,主要研究方向为食品加工与安全。E-mail:987045601@qq.com
  • 1. 仲恺农业工程学院 轻工食品学院/广东省岭南特色食品科学与技术重点实验室, 广东 广州 510225;
  • 2. 广东省梅州市梅县区农业科学研究所, 广东 梅州 514783;
  • 3. 西南大学 食品科学学院, 重庆 400715;
  • 4. 仲恺农业工程学院 现代农业创新研究院, 广东 广州 510225
  • 收稿日期: 2021-06-21
  • 录用日期: 2022-01-19
基金项目:  国家自然科学基金项目(32001622)广东省岭南特色食品科学与技术重点实验室项目(2021B1212040013)国家重点研发计划项目(2019YFD1002300)

摘要: 以柚子幼果为原料,采用亚临界流体萃取技术提取其精油,利用GC-MS联用技术对精油的化学成分进行分析,并应用AAPH诱导红细胞氧化应激模型对精油的抗氧化活性进行初步研究。结果表明:柚子幼果精油中共鉴定出126种有效成分,主要包括醇类、萜烯类、酮类、酯类、醛类、酚类和烷烃类7类化合物,其中醇类为主要化合物,共11种,相对含量为16.43%,萜烯类共10种,相对含量为5.37%,酮类共10种,相对含量为3.71%;经柚子幼果精油预处理后,AAPH诱导的红细胞溶血受到抑制,细胞外的MDA浓度和LDH释放量均减少,在保护细胞膜完整性的同时,也缓解了细胞的氧化应激反应,即柚子幼果精油能够通过清除自由基抑制AAPH引起的红细胞溶血。

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