JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 37 Issue 4
August 2022
Article Contents
LI Min, XU Dongming, YANG Yuwen, et al. A method for determination of heterocyclic aromatic amines in vinegar by ionic liquid aqueous two-phase extraction coupled with UPLC[J]. Journal of Light Industry, 2022, 37(4): 26-33. doi: 10.12187/2022.04.004
Citation: LI Min, XU Dongming, YANG Yuwen, et al. A method for determination of heterocyclic aromatic amines in vinegar by ionic liquid aqueous two-phase extraction coupled with UPLC[J]. Journal of Light Industry, 2022, 37(4): 26-33. doi: 10.12187/2022.04.004 shu

A method for determination of heterocyclic aromatic amines in vinegar by ionic liquid aqueous two-phase extraction coupled with UPLC

  • Received Date: 2021-07-12
    Accepted Date: 2022-04-24
  • A method of ionic liquid aqueous two-phase system, in which optimum extraction conditions were achieved by both single factor experiment and orthogonal experiment, combined with ultra-high performance liquid chromatography (UPLC), was established to detect Harman and Norharmane in vinegar. The experimental results showed that the optimum extraction conditions were as follows: [C6MIM]Br-K2HPO4 were used as the ionic liquid aqueous two-phase system, the amount of [C6MIM]Br was 200 μL, the amount of K2HPO4 was 0.5 g, pH value was 10, and the ultrasound time was 3 min. Under the appropriate optimal conditions, both the linear equations of Harman and Norharmane were 10.0~400.0 μg/mL, and both the correlation coefficients R2 were 0.999 5. The limits of detection (LODs) of Harman and Norharmane were 0.50 μg/mL and 0.45 μg/mL, respectively. All the precision experiment, repeatability experiment, and recovery experiment showed that the method had better accuracy and precision. That is, the method could be used for the determination of Harman and Norharmane in vinegar.
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