JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 37 Issue 4
August 2022
Article Contents
LONG Zhangde, WANG Min, XUE Yun, et al. Screening and identifying of β-carotene degrading strains from tobacco leaves and its optimization of fermentation conditions[J]. Journal of Light Industry, 2022, 37(4): 86-93. doi: 10.12187/2022.04.012
Citation: LONG Zhangde, WANG Min, XUE Yun, et al. Screening and identifying of β-carotene degrading strains from tobacco leaves and its optimization of fermentation conditions[J]. Journal of Light Industry, 2022, 37(4): 86-93. doi: 10.12187/2022.04.012 shu

Screening and identifying of β-carotene degrading strains from tobacco leaves and its optimization of fermentation conditions

  • Received Date: 2021-09-29
  • The strains that could degrade β-carotene with high efficiency were screened from fresh Yuyan 13 tobacco leaves, and the main degradation products were detected by GC-MS, the species of the strain were identified by morphology and 16S rDNA phylogenetic tree, the fermentation conditions of the strain were optimized by single factor test and orthogonal test. The results showed that YT-3 strain was Enterobacter hormanii subsp.which could degrade β-carotene with high efficiency. The main degradation products were β-ionone, dihydrokiwifruit lactone, β-cyclic citral (3.21%) and other flavor substances. The optimum fermentation conditions of YT-3 strain were as follows: NaNO3 concentration 4 g/L, sucrose concentration 30 g/L, yeast powder concentration 3 g/L, initial pH value 7.0. Under these fermentation conditions, the degradation rate of β-carotene reached 97.05%.
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