JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

生物标志物检测方法的研究进展

武丰龙 ,  崔艳英 ,  张志锋 ,  王丹丹

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武丰龙, 崔艳英, 张志锋, 等. 生物标志物检测方法的研究进展[J]. 轻工学报, 2022, 37(5): 50-60. doi: 10.12187/2022.05.006
引用本文: 武丰龙, 崔艳英, 张志锋, 等. 生物标志物检测方法的研究进展[J]. 轻工学报, 2022, 37(5): 50-60. doi: 10.12187/2022.05.006
shu
WU Fenglong, CUI Yanying, ZHANG Zhifeng and et al. Research progress of methods for biomarker detection[J]. Journal of Light Industry, 2022, 37(5): 50-60. doi: 10.12187/2022.05.006
Citation: WU Fenglong, CUI Yanying, ZHANG Zhifeng and et al. Research progress of methods for biomarker detection[J]. Journal of Light Industry, 2022, 37(5): 50-60. doi: 10.12187/2022.05.006
shu

生物标志物检测方法的研究进展

  • 1. 郑州轻工业大学 软件学院, 河南 郑州 450001;

  • 2. 郑州轻工业大学 计算机与通信工程学院, 河南 郑州 450001

    • 作者简介: 武丰龙(1976-),男,河南省南阳市人,郑州轻工业大学讲师,主要研究方向为智能信息检测与信息计算。E-mail:wufenglong1112@163.com;

  • 基金项目: 国家自然科学基金面上基金项目(61975187)

  • 中图分类号: TS207.4;R318

Research progress of methods for biomarker detection

  • 1. College of Software Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;

  • 2. College of Computer and Communication Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China

  • Received Date: 2021-06-11
    Accepted Date: 2022-01-20

    CLC number: TS207.4;R318

  • 摘要: 综述了基于遗传物质(RNA和DNA)、免疫学(抗原/抗体)及结合微芯片技术的生物标志物检测方法的应用研究进展,指出:基于遗传物质的检测方法直接作用于靶标物质,准确性和灵敏度高,但其检测步骤繁多,对检测环境要求高;基于免疫学的检测方法操作简单、便携性高、能实现即时检测但准确性稍低,适用于快速、大规模的病毒筛查;结合微芯片技术的检测方法更加多元化,并能在短时间内实现靶标物质的检测,精确性、自动化程度得到提高,在病毒筛查、疾病诊断等方面表现出巨大的发展潜力。未来生物标志物检测技术可就优化免疫催化反应物、加大核酸提取物纯化程度、提高检测设备自动化程度、构建多重检测系统、实现检测设备微型化及检测信息可视化等方面开展进一步研究,以提高检测准确性、设备便携性,从而实现该技术的持续发展。
    Abstract: The application research progress of biomarker detection methods based on genetic material (RNA and DNA), immunology (antigen/antibody) and combined with microchip technology was reviewed. It was pointed out that the detection method based on genetic material acted directly on the target substance, and had high accuracy and good sensitivity, but it had many detection steps and high requirements for the detection environment; The detection method based on immunology was simple to operate, had high portabihity, and could realize instant detection. However, the accuracy was slightly lower. It was suitable for rapid and large-scale virus screening; Combining with microchip technology made the detection method more diverse. It could realize the detection of target substances in a short time, and the accuracy and degree of automation have been improved. This method showed great development potential in virus screening and disease diagnosis. In the future, the detection technology of biomarkers could be further studied in terms of optimizing the catalyst of the reaction, increasing the degree of purification of nucleic acid extracts, improving the automation of detection equipment, building multiple detection systems, realizing the miniaturization of detection equipment and visualization of detection information, etc. to improve detection accuracy and equipment portability so as to realize the continuous development of this technology.
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武丰龙, 崔艳英, 张志锋, 等. 生物标志物检测方法的研究进展[J]. 轻工学报, 2022, 37(5): 50-60. doi: 10.12187/2022.05.006
引用本文: 武丰龙, 崔艳英, 张志锋, 等. 生物标志物检测方法的研究进展[J]. 轻工学报, 2022, 37(5): 50-60. doi: 10.12187/2022.05.006
shu
WU Fenglong, CUI Yanying, ZHANG Zhifeng and et al. Research progress of methods for biomarker detection[J]. Journal of Light Industry, 2022, 37(5): 50-60. doi: 10.12187/2022.05.006
Citation: WU Fenglong, CUI Yanying, ZHANG Zhifeng and et al. Research progress of methods for biomarker detection[J]. Journal of Light Industry, 2022, 37(5): 50-60. doi: 10.12187/2022.05.006
shu

生物标志物检测方法的研究进展

    作者简介:武丰龙(1976-),男,河南省南阳市人,郑州轻工业大学讲师,主要研究方向为智能信息检测与信息计算。E-mail:wufenglong1112@163.com
  • 1. 郑州轻工业大学 软件学院, 河南 郑州 450001;
  • 2. 郑州轻工业大学 计算机与通信工程学院, 河南 郑州 450001
  • 收稿日期: 2021-06-11
  • 录用日期: 2022-01-20
基金项目:  国家自然科学基金面上基金项目(61975187)

摘要: 综述了基于遗传物质(RNA和DNA)、免疫学(抗原/抗体)及结合微芯片技术的生物标志物检测方法的应用研究进展,指出:基于遗传物质的检测方法直接作用于靶标物质,准确性和灵敏度高,但其检测步骤繁多,对检测环境要求高;基于免疫学的检测方法操作简单、便携性高、能实现即时检测但准确性稍低,适用于快速、大规模的病毒筛查;结合微芯片技术的检测方法更加多元化,并能在短时间内实现靶标物质的检测,精确性、自动化程度得到提高,在病毒筛查、疾病诊断等方面表现出巨大的发展潜力。未来生物标志物检测技术可就优化免疫催化反应物、加大核酸提取物纯化程度、提高检测设备自动化程度、构建多重检测系统、实现检测设备微型化及检测信息可视化等方面开展进一步研究,以提高检测准确性、设备便携性,从而实现该技术的持续发展。

English Abstract

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