JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Chaetomium globosum CGMCC 6882发酵所产黄酮类化合物对小鼠生长和肠道菌群的影响

王璐 ,  范伊琳 ,  王子朝 ,  郭佳源 ,  王琦 ,  牛梦 ,  师怡宁

downloadPDF
王璐, 范伊琳, 王子朝, 等. Chaetomium globosum CGMCC 6882发酵所产黄酮类化合物对小鼠生长和肠道菌群的影响[J]. 轻工学报, 2022, 37(6): 34-41. doi: 10.12187/2022.06.005
引用本文: 王璐, 范伊琳, 王子朝, 等. Chaetomium globosum CGMCC 6882发酵所产黄酮类化合物对小鼠生长和肠道菌群的影响[J]. 轻工学报, 2022, 37(6): 34-41. doi: 10.12187/2022.06.005
shu
WANG Lu, FAN Yilin, WANG Zichao, et al. Effects of flavonoids produced by Chaetomium globosum CGMCC 6882 on the body weight and gut microbiota of mice[J]. Journal of Light Industry, 2022, 37(6): 34-41. doi: 10.12187/2022.06.005
Citation: WANG Lu, FAN Yilin, WANG Zichao, et al. Effects of flavonoids produced by Chaetomium globosum CGMCC 6882 on the body weight and gut microbiota of mice[J]. Journal of Light Industry, 2022, 37(6): 34-41. doi: 10.12187/2022.06.005
shu

Chaetomium globosum CGMCC 6882发酵所产黄酮类化合物对小鼠生长和肠道菌群的影响

  • 1. 河南工业大学 生物工程学院, 河南 郑州 450001;

  • 2. 华商国际工程有限公司, 北京 100069;

  • 3. 河南工业大学 国家工程实验室/省重点实验室, 河南 郑州 450001;

  • 4. 河南工业大学 国际教育学院, 河南 郑州 450001

    • 作者简介: 王璐(1998—),女,黑龙江省齐齐哈尔市人,河南工业大学硕士研究生,主要研究方向为微生物学。E-mail:wangl201906@163.com;

  • 基金项目: 河南工业大学青年骨干教师培育计划项目(21420116)
    河南工业大学粮油食品学科国家工程实验室/省重点实验室项目(NL2021006)
    河南省自然科学基金项目(212300410131)

  • 中图分类号: TS201.1

Effects of flavonoids produced by Chaetomium globosum CGMCC 6882 on the body weight and gut microbiota of mice

  • 1. School of Biological Engineering, He'nan University of Technology, Zhengzhou 450001, China;

  • 2. Huashang International Engineering Co., Ltd., Beijing 100069, China;

  • 3. National Engineering Laboratory/Key Laboratory of He'nan Province, He'nan University of Technology, Zhengzhou 450001, China;

  • 4. School of International Education, He'nan University of Technology, Zhengzhou 450001, China

  • Received Date: 2022-01-24

    CLC number: TS201.1

  • 摘要: 以绞股蓝内生真菌CGMCC 6882(Chaetomium globosum CGMCC 6882)发酵所产黄酮类化合物为研究对象,通过检测小鼠生长情况、体重、血清生化指标、肠道菌群组成及代谢物短链脂肪酸(SCFAs),探究其对小鼠生长和肠道菌群的影响。结果表明:饲养21 d后,空白对照组小鼠净增体重为9.5 g,实验组小鼠净增体重为7.2 g,血清生化指标无明显变化;随饲养时间的延长,实验组小鼠Ace指数、Chao1指数与Shannon指数均上升、Simpson指数下降,OTU数也高于对照组;对照组小鼠肠道中Firmicutes/Bacteroidetes(F/B)值均在0.74以上,而实验组小鼠F/B值在0.41以下,且实验组小鼠肠道中乙酸、丙酸、丁酸和总酸含量分别由22.1 μmol/g、9.3 μmol/g、8.1 μmol/g和39.5 μmol/g上升至29.3 μmol/g、15.2 μmol/g、9.5 μmol/g和54.0 μmol/g。因此,C.globosum CGMCC 6882发酵所产黄酮类化合物可以有效降低小鼠体重、提高肠道菌群多样性和丰度、增加肠道中SCFAs含量。
    Abstract: Flavonoids produced by C.globosum CGMCC 6882 was used to detect the effects on mice gut microbiota via analyzing the growth profile, body weight, serum indexes, gut microbiota composition and short-chain fatty acids (SCFAs). Results showed that after 21 days feeding, body weight gain of mice in control group was 9.5 g and the body weight gain in experimental group was 7.2 g, and there was no significant change in serum biochemical indexes. Compared to control group, Ace, Chao1 and Shannon indexes and OTU number in flavonoids group increased with the extension of feeding time, but Simpson index decreased. In addition, the values of Firmicutes/Bacteroidetes (F/B) in control were higher than 0.74, while the F/B values of flavonoids group were lower than 0.41. Furthermore, acetate, propionate, butyrate and total short chain fatty acids in the cecum contents of flavonoids group mice increased from 22.1 μmol/g, 9.3 μmol/g, 8.1 μmol/g and 39.5 μmol/g to 29.3 μmol/g, 15.2 μmol/g, 9.5 μmol/g and 54.0 μmol/g, respectively. This work indicated that flavonoids produced by C.globosum CGMCC 6882 could not only effectively reduce the body weight of mice, but also increase the diversity, abundance and SCFAs contents of gut microbiota of mice.
    1. [1]

      TIAN B M,ZHAO J H,AN W,et al.Lycium ruthenicum diet alters the gut microbiota and partially enhances gut barrier function in male C57BL/6 mice [J].Journal of Functional Foods, 2019,52:516-528.

    2. [2]

      GONZÍLEZ-PARAMÁS A M,AYUDA-DURÁN B,MARTÍNEZ S,et al.The mechanisms behind the biological activity of flavonoids [J].Current Medicinal Chemistry,2019,26(39):6976-6990.

    3. [3]

      肖咏梅,李明,毛璞,等.黄酮类化合物生物改性及活性的研究进展[J].河南工业大学学报(自然科学版),2019,40(2):123-131,139.

    4. [4]

      BAGHERI N,AL LAWATI H A J,HASSANZADEH J.Simultaneous determination of total phenolic acids and total flavonoids in tea and honey samples using an integrated lab on a chip device [J].Food Chemistry,2021,342:128338.

    5. [5]

      JENNINGS A,KOCH M,BANG C,et al.Microbial diversity and abundance of parabacteroides mediate the associations between higher intake of flavonoid-rich foods and lower blood pressure [J].Hypertension,2021,78:1016-1026.

    6. [6]

      PEI R S,LIU X C,BOLLING B.Flavonoids and gut health[J].Current Opinion in Biotechnology,2020,61:153-159.

    7. [7]

      李明,卢慧,徐振江.黄酮类天然产物调控肠道微生物改善炎症性肠病的研究进展[J].河南工业大学学报(自然科学版),2020,41(3):118-129.

    8. [8]

      CHEN B,LUO J K,HAN Y H,et al.Dietary tangeretin alleviated dextran sulfate sodium-induced colitis in mice via inhibiting inflammatory response,restoring intestinal barrier function,and modulating gut microbiota[J].Journal of Agricultural and Food Chemistry,2021,69:7663-7674.

    9. [9]

      白利琴.多糖黄酮对大鼠血脂代谢与肠道菌群调节作用研究[D].石家庄:河北科技大学,2020.

    10. [10]

      HOEK-VAN DEN HIL E F,VAN SCHOTHORST E M,VAN DER STELT I,et al.Direct comparison of metabolic health effects of the flavonoids quercetin,hesperetin,epicatechin,apigenin and anthocyanins in high-fat-diet-fed mice [J].Genesand Nutrition,2015,10(4):23.

    11. [11]

      PANDA V,SHINDE P.Appetite suppressing effect of Spinacia oleracea in rats:Involvement of the short term satiety signal cholecystokinin [J].Appetite,2017,113:224-230.

    12. [12]

      付乾振,梁小娜,郑欣欣,等.Chaetomium globosum CGMCC 6882产黄酮类化合物的发酵工艺及产物抗氧化活性研究[J].河南工业大学学报(自然科学版),2022,43(1):83-90.

    13. [13]

      王伟,何平,江小明.木犀草素及其黄酮苷的抗炎、抗氧化作用[J].食品科学,2020,41(17):208-215.

    14. [14]

      GAO S Y,ZHAO C N,XU X Y,et al.Dietary plants,gut microbiota,and obesity:Effects and mechanisms[J].Trends in Food Science & Technology,2019,92(C):194-204.

    15. [15]

      周敬凯,翁竞玉,朱盼,等.地菍总黄酮对高脂饮食大鼠减肥作用及肠道菌群的影响[J].中成药,2021,43(10):2673-2678.

    16. [16]

      马欣雨,徐非,龚蕾蕾,等.王不留行黄酮苷对小鼠动脉粥样硬化的作用及机制[J].中国药理学通报,2021,37(11):1518-1523.

    17. [17]

      DUAN R Q,GUAN X,HUANG K,et al.Flavonoids from whole-grain oat alleviated high-fat diet-induced hyperlipidemia via regulating bile acid metabolism and gut microbiota in mice[J].Journal of Agricultural and Food Chemistry,2021,69:7629-7640.

    18. [18]

      ZHAO R Q,YANG W J,PEI F,et al.In vitro fermentation of six kinds of edible mushrooms and its effects on fecal microbiota composition[J].LWT-Food Science and Technology,2018,96:627-635.

    19. [19]

      王方杰,吴祖芳,翁佩芳,等.胡柚黄酮对高脂饮食诱导的肥胖小鼠模型肠道菌群的调节作用[J].食品科学,2020,41(21):140-146.

    20. [20]

      TURNBAUGH P J,HAMADY M,YATSUNENKO T,et al.A core gut microbiome in obese and lean twins[J].Nature,2009,457:480-484.

    21. [21]

      KOLIADA A,SYZENKO G,MOSEIKO V,et al.Association between body mass index and Firmicutes/Bacteroidetes ratio in an adult Ukrainian population[J].BMC Microbiology,2017,17:1017-1027.

    22. [22]

      LEY R E,BÄCKHED F,TURNBAUGH P,et al.Obesity alters gut microbial ecology[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102:11070-11075.

    23. [23]

      GAO R Y,ZHU C L,LI H,et al.Dysbiosis signatures of gut microbiota along the sequence from healthy,young patients to those with overweight and obesity[J].Obesity,2018,26:351-361.

    24. [24]

      MORRISON D J,PRESTON T.Formation of short chain fatty acids by the gut microbiota and their impact on human metabolism[J].Gut Microbes,2016,7:189-200.

    25. [25]

      LU Y Y,FAN C N,LI P,et al.Short chain fatty acids prevent high-fat-diet-induced obesity in mice by regulating G protein-coupled receptors and gut microbiota[J].Scientific Reports,2016,6:37589.

    26. [26]

      CANFORA E E,JOCKEN J W,BLAAK E E.Short-chain fatty acids in control of body weight and insulin sensitivity[J].Nature Reviews Endocrinology,2015,11:577-591.

    1. [1]

      李敏 , 贺姗姗 , 杨钰雯 . 改良QuEChERS方法结合超高效液相色谱测定火腿肠中杂环胺类化合物. 轻工学报, 2024, 39(5): 60-70. doi: 10.12187/2024.05.007

  • 加载中
WeChat 点击查看大图
计量
  • PDF下载量:  14
  • 文章访问数:  1312
  • 引证文献数: 0
文章相关
  • 收稿日期:  2022-01-24
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
王璐, 范伊琳, 王子朝, 等. Chaetomium globosum CGMCC 6882发酵所产黄酮类化合物对小鼠生长和肠道菌群的影响[J]. 轻工学报, 2022, 37(6): 34-41. doi: 10.12187/2022.06.005
引用本文: 王璐, 范伊琳, 王子朝, 等. Chaetomium globosum CGMCC 6882发酵所产黄酮类化合物对小鼠生长和肠道菌群的影响[J]. 轻工学报, 2022, 37(6): 34-41. doi: 10.12187/2022.06.005
shu
WANG Lu, FAN Yilin, WANG Zichao, et al. Effects of flavonoids produced by Chaetomium globosum CGMCC 6882 on the body weight and gut microbiota of mice[J]. Journal of Light Industry, 2022, 37(6): 34-41. doi: 10.12187/2022.06.005
Citation: WANG Lu, FAN Yilin, WANG Zichao, et al. Effects of flavonoids produced by Chaetomium globosum CGMCC 6882 on the body weight and gut microbiota of mice[J]. Journal of Light Industry, 2022, 37(6): 34-41. doi: 10.12187/2022.06.005
shu

Chaetomium globosum CGMCC 6882发酵所产黄酮类化合物对小鼠生长和肠道菌群的影响

    作者简介:王璐(1998—),女,黑龙江省齐齐哈尔市人,河南工业大学硕士研究生,主要研究方向为微生物学。E-mail:wangl201906@163.com
  • 1. 河南工业大学 生物工程学院, 河南 郑州 450001;
  • 2. 华商国际工程有限公司, 北京 100069;
  • 3. 河南工业大学 国家工程实验室/省重点实验室, 河南 郑州 450001;
  • 4. 河南工业大学 国际教育学院, 河南 郑州 450001
  • 收稿日期: 2022-01-24
基金项目:  河南工业大学青年骨干教师培育计划项目(21420116)河南工业大学粮油食品学科国家工程实验室/省重点实验室项目(NL2021006)河南省自然科学基金项目(212300410131)

摘要: 以绞股蓝内生真菌CGMCC 6882(Chaetomium globosum CGMCC 6882)发酵所产黄酮类化合物为研究对象,通过检测小鼠生长情况、体重、血清生化指标、肠道菌群组成及代谢物短链脂肪酸(SCFAs),探究其对小鼠生长和肠道菌群的影响。结果表明:饲养21 d后,空白对照组小鼠净增体重为9.5 g,实验组小鼠净增体重为7.2 g,血清生化指标无明显变化;随饲养时间的延长,实验组小鼠Ace指数、Chao1指数与Shannon指数均上升、Simpson指数下降,OTU数也高于对照组;对照组小鼠肠道中Firmicutes/Bacteroidetes(F/B)值均在0.74以上,而实验组小鼠F/B值在0.41以下,且实验组小鼠肠道中乙酸、丙酸、丁酸和总酸含量分别由22.1 μmol/g、9.3 μmol/g、8.1 μmol/g和39.5 μmol/g上升至29.3 μmol/g、15.2 μmol/g、9.5 μmol/g和54.0 μmol/g。因此,C.globosum CGMCC 6882发酵所产黄酮类化合物可以有效降低小鼠体重、提高肠道菌群多样性和丰度、增加肠道中SCFAs含量。

English Abstract

参考文献 (26) 相关文章 (1)

目录

/

返回文章

网站版权 © 轻工学报编辑部

地址：河南省郑州市科学大道136号邮编：450001

本系统由北京仁和汇智信息技术有限公司开发 技术支持： info@rhhz.net