安石榴苷对UVB诱导HaCaT细胞光损伤的保护作用

沈芳铭; 尹淑涛; 赵冲

doi:10.12187/2022.06.007

安石榴苷对UVB诱导HaCaT细胞光损伤的保护作用

中国农业大学食品科学与营养工程学院, 北京 100083

作者简介: 沈芳铭(1999—),女,福建省泉州市人,中国农业大学硕士研究生,主要研究方向为营养与食品安全。E-mail:shenfangming7123@163.com;

基金项目: 国家自然科学基金青年科学基金项目(31901691)
中图分类号: TS255.1

Protective effect of punicalagin on UVB-induced photodamage in HaCaT cells

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Received Date: 2022-05-30

CLC number: TS255.1

摘要: 利用Caco-2细胞和HaCaT细胞建立肠-皮肤共培养体系,采用免疫荧光法评价安石榴苷对中波紫外线(UVB)诱导的光损伤产物环丁烷嘧啶二聚体(CPD)的抑制效果,通过实时荧光定量PCR、免疫沉淀、蛋白免疫印迹法探究安石榴苷对核苷酸切除修复(NER)途径相关基因转录和产物表达的影响。结果表明,安石榴苷能降低HaCaT细胞中的CPD水平,上调着色性干皮病基因组C(XPC)的mRNA转录水平,并以SIRT1基因依赖的方式降低XPA蛋白的乙酰化水平。这表明在Caco-2细胞与HaCaT细胞的肠-皮肤共培养体系中,安石榴苷可能通过激活NER途径相关的XPC/XPA蛋白促进UVB诱导的光损伤的修复,其作用依赖于SIRT1蛋白的去乙酰化活性。
- 安石榴苷 /
- 中波紫外线 /
- 细胞共培养 /
- 光损伤 /
- 核苷酸切除修复
Abstract: In this study, the co-culture system of Caco-2 and HaCaT cells was established, and the inhibitory effect of punicalagin on cyclobutene pyrimidine dimers (CPD) which was one major type of UVB-induced DNA lesions was evaluated by immunofluorescence. Then the effects of punicalagin on transcription and expression levels of nucleotide excision repair (NER) related genes was further investigated by quantitative real-time PCR, immunoprecipitation and western-blotting. The results showed that punicalagin reduced CPD level in HaCaT cells, upregulated xeroderma pigmentosum group C (XPC) mRNA level and attenuated XPA acetylation in a SIRT1-dependent manner. This showed, punicalagin's efficacy against UVB-induced photodamage in the co-culture system of Caco-2 and HaCaT cells was probably activated by the NER related XPC/XPA which depended on the deacetylation activity of SIRT1.
- punicalagin /
- ultraviolet B /
- co-culture /
- photodamage /
- nucleotide excision repair
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安石榴苷对UVB诱导HaCaT细胞光损伤的保护作用

作者简介: 沈芳铭(1999—),女,福建省泉州市人,中国农业大学硕士研究生,主要研究方向为营养与食品安全。E-mail:shenfangming7123@163.com;

Protective effect of punicalagin on UVB-induced photodamage in HaCaT cells

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通讯作者: 陈斌, bchen63@163.com

安石榴苷对UVB诱导HaCaT细胞光损伤的保护作用

作者简介:沈芳铭(1999—),女,福建省泉州市人,中国农业大学硕士研究生,主要研究方向为营养与食品安全。E-mail:shenfangming7123@163.com

English Abstract

Protective effect of punicalagin on UVB-induced photodamage in HaCaT cells

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安石榴苷对UVB诱导HaCaT细胞光损伤的保护作用

作者简介: 沈芳铭(1999—),女,福建省泉州市人,中国农业大学硕士研究生,主要研究方向为营养与食品安全。E-mail:shenfangming7123@163.com;

Protective effect of punicalagin on UVB-induced photodamage in HaCaT cells

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通讯作者: 陈斌, bchen63@163.com

安石榴苷对UVB诱导HaCaT细胞光损伤的保护作用

作者简介:沈芳铭(1999—),女,福建省泉州市人,中国农业大学硕士研究生,主要研究方向为营养与食品安全。E-mail:shenfangming7123@163.com

English Abstract

Protective effect of punicalagin on UVB-induced photodamage in HaCaT cells

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