JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 38 Issue 1
February 2023
Article Contents
WANG Anna, WANG Xuebing and WU Ligen. Effect of ionic concentration on the interaction between gelatin and amylose[J]. Journal of Light Industry, 2023, 38(1): 1-9. doi: 10.12187/2023.01.001
Citation: WANG Anna, WANG Xuebing and WU Ligen. Effect of ionic concentration on the interaction between gelatin and amylose[J]. Journal of Light Industry, 2023, 38(1): 1-9. doi: 10.12187/2023.01.001 shu

Effect of ionic concentration on the interaction between gelatin and amylose

  • Received Date: 2022-06-07
  • The effect of ionic concentration on the interaction between gelatin and amylose was investigated by spectroscopic methods. The results showed that ionic concentration could change the hydrophobic microenvironment of gelatin. With different ionic concentration, amylose could quench the endogenous fluorescence of gelatin to different degrees. The quenching mechanism was coupling quenching, which was mainly static quenching. With the increase of ionic concentration, the binding ability of gelatin and amylose was enhanced. In aqueous solution, the forces between gelatin and amylose were mainly van der Waals forces and hydrogen bonds, which were mainly driven by hydrophobic forces after sodium chloride was added. Gelatin-amylose binding caused rearrangement and conformational changes of gelatin, resulting in the change of tyrosine residues and tryptophan residues, and the binding site was closer to the tyrosine residues. The gelatin-amylose interaction was effected by the ionic concentration, the addition of sodium chloride was beneficial to the combination of gelatin and amylose and caused the change of conformation of gelatin.
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