JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 38 Issue 1
February 2023
Article Contents
HUANG Shen, LIU Danyang, ZHANG Zenghui, et al. Study on improving tobacco quality mechanism of Shandong province by Enterobacter and Acinetobacter mixed fermentation[J]. Journal of Light Industry, 2023, 38(1): 45-53. doi: 10.12187/2023.01.006
Citation: HUANG Shen, LIU Danyang, ZHANG Zenghui, et al. Study on improving tobacco quality mechanism of Shandong province by Enterobacter and Acinetobacter mixed fermentation[J]. Journal of Light Industry, 2023, 38(1): 45-53. doi: 10.12187/2023.01.006 shu

Study on improving tobacco quality mechanism of Shandong province by Enterobacter and Acinetobacter mixed fermentation

  • Received Date: 2022-11-03
  • Shandong tobacco leaves were fermented by Enterobacter cloacae F8-2 and Acinetobacter nosocomialis 7S-2 mixed bacteria. The sensory quality, chemical composition and chemical composition of tobacco smoke and microbial community on tobacco leaf surface before and after fermentation were studied. The results showed that when Enterobacter and Acinetobacter were mixed in 1:1 fermentation for 24 h, the aroma of the tobacco leaf increased and the impurity of the tobacco leaf decreased most significantly. After fermentation, methylheptenone, dihydrotomarone and 5-methylfurfural increased by 89.22%, 35.67% and 33.93%, respectively. Nerolol, solanone and acyl alcohol in tobacco smoke increased by 78.01%, 46.83% and 45.95%, respectively. Mixed fermentation had a great impact on the bacterial community on the surface of tobacco leaves, and the microorganisms cooperated with each other in fermentation and symbiosis. At the same time, Enterobacter and Acinetobacter may be related to the production of aromatic substances such as benzaldehyde and methyl heptene ketone.
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    1. [1]

      REID J J, MCKINSTRY D W, HALEY D E.The fermentation of cigar-leaf tobacco[J].Science, 1937, 86(2235):404.

    2. [2]

      ZHENG T F, ZHANG Q Y, WU Q Y, et al.Effects of inoculation with acinetobacter on fermentation of cigar tobacco leaves[J].Front Microbiol, 2022, 13:911791.

    3. [3]

      DAI J C, DONG A J, XIONG G X, et al.Production of highly active extracellular amylase and cellulase from Bacillus subtilis ZIM3 and a recombinant strain with a potential application in tobacco fermentation[J].Front Microbiol, 2020, 11:1539.

    4. [4]

      ZANG J H, XU Y S, XIA W S, et al.Correlations between microbiota succession and flavor formation during fermentation of Chinese low-salt fermented common carp(Cyprinus carpio L.) inoculated with mixed starter cultures[J].Food Microbiol, 2020, 90:103487.

    5. [5]

      徐颖宣, 徐尔尼, 冯乃宪, 等.微生物混菌发酵应用研究进展[J].中国酿造, 2008(5):1-4.

    6. [6]

      龙章德, 苏赞, 李季刚, 等.一种改善广西百色地区烟叶品质的混合微生物发酵技术[J].轻工学报, 2021, 36(5):59-66.

    7. [7]

      司美茹, 薛泉宏, 蔡艳.混合发酵对纤维素酶和淀粉酶活性的影响[J].西北农林科技大学学报, 2002(5):69-73.

    8. [8]

      薛云, 宁振兴, 苏赞, 等.基于芽孢杆菌固态发酵技术提高广西河池C4F烟叶品质的研究[J].轻工学报, 2022, 37(5):76-84.

    9. [9]

      韩锦峰, 朱大恒, 刘卫群, 等.陈化发酵期间烤烟叶面微生物活性及其应用研究[J].中国烟草科学, 1997(4):15-16.

    10. [10]

      帅瑶, 陶菡, 田运霞, 等.复合菌种发酵烟叶产酶及挥发性风味物质变化[J].河南农业科学, 2020, 49(10):162-175.

    11. [11]

      李萌, 王旭东, 罗昭标, 等.混菌固态发酵低次烟叶工艺优化及挥发性致香成分分析[J].河南农业科学, 2022, 51(9):171-180.

    12. [12]

      WU X Y, ZHU P C, LI D L, et al.Bioaugmentation of Bacillus amyloliquefaciens-Bacillus kochii co-cultivation to improve sensory quality of flue-cured tobacco[J].Arch Microbiol, 2021, 203:5723-5733.

    13. [13]

      国家烟草专卖局.卷烟用常规分析用吸烟机测定总粒相物和焦油:GB/T 19609-2004[S].北京:中国标准出版社, 2004.

    14. [14]

      毛多斌, 黄晓玉, 周利峰, 等.枯草芽孢杆菌分离鉴定及其对烟叶化学成分和吸味品质的影响[J].烟草科技, 2022, 55(8):10-19.

    15. [15]

      郑泽浩.烟叶微生物多样性及其对烟叶发酵品质的影响研究[D].咸阳:西北农林科技大学, 2020.

    16. [16]

      赵铭钦, 李芳芳, 李晓强, 等.不同生物制剂处理对发酵烤烟香气物质含量的影响研究[J].云南农业大学学报, 2007(4):519-524.

    17. [17]

      叶建斌, 齐晓娜, 杨宗灿, 等.肠杆菌发酵对烟草浸膏化学成分及卷烟品质的影响[J].食品与机械, 2021, 37(5):33-38.

    18. [18]

      汪芳芳.霍氏肠杆菌降解叶黄素的研究[D].郑州:郑州轻工业学院, 2017.

    19. [19]

      张启东, 刘俊辉, 柴国璧, 等.主流烟气粒相物水溶性组分中烤甜香成分分析[J].烟草科技, 2014(6):54-59.

    20. [20]

      范武, 何峰, 姬凌波, 等.卷烟主流烟气辛香特征成分组群的分布特征及感官贡献[J].烟草科技, 2021, 54(2):36-43.

    21. [21]

      代丽, 黄永成, 宫长荣, 等.密集式烘烤条件下不同变黄温湿度对烤后烟叶致香物质的影响[J].华北农学报, 2008, 23(6):148-152.

    22. [22]

      LI J J, ZHAO Y Y, QIN Y Q, et al.Influence of microbiota and metabolites on the quality of tobacco during fermentation[J].BMC Microbiol, 2020, 20:356.

    23. [23]

      HUANG J W, YANG J K, DUAN Y Q, et al.Bacterial diversities on unaged and aging flue-cured tobacco leaves estimated by 16S rRNA sequence analysis[J].Appl Microbiol Biotechnol, 2010, 88:553-562.

    24. [24]

      WANG F, ZHAO H W, XIANG H Y, et al.Species diversity and functional prediction of surface bacterial communities on aging flue-cured tobaccos[J].Curr Microbiol, 2018, 75:1306-1315.

    25. [25]

      ZHOU J X, YU L F, ZHANG J, et al.Characterization of the core microbiome in tobacco leaves during aging[J].MicrobiologyOpen, 2020, 9(1):e984.

    26. [26]

      ZHAO D D, HU J, CHEN W X.Analysis of the relationship between microorganisms and flavourdevelopment in dry-cured grass carp by high-throughput sequencing, volatile flavour analysis and metabolomics[J].Food Chemistry, 2022, 368:130889.

    27. [27]

      KUMAR M, JI B Y, ZENGLER K, et al.Modeling approaches for studying the microbiome[J].Nat Microbiol, 2014, 4:1253-1267.

    28. [28]

      ZHOU J X, YU L F, ZHANG J, et al.Dynamic characteristics and co-occurrence patterns of microbial community in tobacco leaves during the 24-month aging process[J].Ann Microbiol, 2021, 71:9.

    29. [29]

      WU Q, KONG Y, XU Y.Flavor profile of Chinese liquor is altered by interactions of intrinsic and extrinsic microbes[J].Appl Environ Microbiol, 2016, 82:422-430.

    30. [30]

      HU Y Y, ZHANG L, LIU Q, et al.The potential correlation between bacterial diversity and the characteristic volatile flavour of traditional dry sausages from Northeast China[J].Food Microbiology, 2020, 91:103-505.

    31. [31]

      XU X X, WU B B, ZHAO W T, et al.Correlation between autochthonous microbial communities and key odorants during the fermentation of red pepper (Capsicum annuum L.)[J].Food Microbiology, 2020, 91:103-510.

    32. [32]

      ZHANG Q Y, GENG Z Z, LI D L, et al.Characterization and discrimination of microbial community and co-occurrence patterns in fresh and strong flavor style flue-cured tobacco leaves[J].MicrobiologyOpen, 2020, 9(7):e965.

    33. [33]

      ABBOTT D W, BORASTON A B.Structural biology of pectin degradation by Enterobacteriaceae[J].Microbiol Mol Biol Rev, 2008, 72(2):301-316.

    34. [34]

      RACHMAN M A, FURUTANI Y, NAKASHIMADA Y, et al.Enhanced hydrogen production in altered mixed acid fermentation of glucose by Enterobacer aerogenes[J].J Ferment Bioeng, 1997, 83(4):358-363.

    35. [35]

      ZHENG T F, ZHANG Q Y, PENG Z.et al.Metabolite-based cell sorting workflow for identifying microbes producing carbonyls in tobacco leaves[J].Appl Microbiol Biotechnol, 2022, 106:4199-4209.

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