复合菌群协同发酵烟梗的降解特性及微生物多样性研究

宋丽丽; 霍姗浩; 孙永威; 杨旭; 张志平; 张靖楠; 魏涛

doi:10.12187/2023.01.008

February 2023

Article Contents

SONG Lili, HUO Shanhao, SUN Yongwei, et al. Study on degradation characteristics and microbial diversity of tobacco stem by synergistic fermentation with microbial community[J]. Journal of Light Industry, 2023, 38(1): 63-70. doi: 10.12187/2023.01.008

Citation: SONG Lili, HUO Shanhao, SUN Yongwei, et al. Study on degradation characteristics and microbial diversity of tobacco stem by synergistic fermentation with microbial community[J]. Journal of Light Industry, 2023, 38(1): 63-70. doi: 10.12187/2023.01.008 shu

Study on degradation characteristics and microbial diversity of tobacco stem by synergistic fermentation with microbial community

College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China

Received Date: 2022-10-06

Abstract

The bacterial community composed by Bacillus subtilis and Lactobacillus plantarum was used to ferment tobacco stem, the degradation of cell wall components, the structural properties and thermal reaction characteristics, the changes of aroma components and bacterial community structure of tobacco stem by solid-state anaerobic fermentation were studied. Results showed that after 5 days of anaerobic fermentation, the degradation rate of lignin in tobacco stem was 50.55%. Degradation of lignin was enhanced mainly by the breakdown of covalent bond between lignin and hemicellulose, demethylation, benzene ring decomposition, etc. After anaerobic fermentation, the pyrolysis weight loss of tobacco stem was increased by 4.85% and the maximum pyrolysis rate was increased by 13.28%, which was more conducive to the pyrolysis reaction. The content of total aroma components in tobacco stem after fermentation was increased by 7.88%, and the content of degraded carotene products increased significantly. In addition to Bacillus subtilis and Lactobacillus plantarum, the relative abundance of functional bacteria related to lignin degradation was greatly increased, and the degradation and transformation of tobacco stem lignin was improved by the synergistic fermentation with microbial community.
- tobacco stem,
- lignin,
- bacterial community,
- solid-state anaerobic fermentation,
- aroma component
References
1. [1]
  ZOU X D, BK A, ABU-IZNEID T, et al.Current advances of functional phytochemicals in Nicotiana plant and related potential value of tobacco processing waste:A review[J].Biomedicine and Pharmacotherapy, 2021, 143:112191.
2. [2]
  杨旭, 魏涛, 张志平, 等.强化微生物固态厌氧发酵对烟梗细胞壁组分的影响研究[J].轻工学报, 2020, 35(6):35-40.
3. [3]
  WANG Y F, CHEN K F, MO L H, et al.Optimization of coagulation-flocculation process for papermaking-reconstituted tobacco slice wastewater treatment using response surface methodology[J].Journal of Industrial and Engineering Chemistry, 2014, 20(2):391-396.
4. [4]
  侯轶, 胡亚成, 李友明, 等.碱性离子液体有效去除烟梗中木质素的研究[J].湖南大学学报(自然科学版), 2017, 44(12):150-156.
5. [5]
  米兰, 王保兴, 周桂园, 等.再造烟叶烟梗原料化学成分及梗膏感官质量分析[J].湖北农业科学, 2019, 58(5):80-84.
6. [6]
  WANG L, WEN Y B, SUN D P, et al.Study on the decrease of harmful substance in paper-process reconstituted tobacco sheet[C]//Advanced Materials Research. Stafa-Zurich:Trans Tech Publications Ltd, 2011:2338-2343.
7. [7]
  KHAN M U, AHRING B K.Lignin degradation under anaerobic digestion:Influence of lignin modifications-A review[J].Biomass and Bioenergy, 2019, 128:105325.
8. [8]
  NAGLE N J, DONOHOE B S, WOLFRUM E J, et al.Chemical and structural changes in corn stover after ensiling:Influence on bioconversion[J].Frontiers in Bioengineering and Biotechnology, 2020, 8:739.
9. [9]
  甄达文, 于铁妹, 朱珊珊, 等.利用酶法与微生物发酵法制备天然烟用香料[J].食品工业科技, 2009, 30(12):268-272.
10. [10]
  DINESH G H, SUNDARAM K, MOHANRASU K, et al. Optimization (substrate and pH) and anaerobic fermentative hydrogen production by various industrial wastes isolates utilizing biscuit industry waste as substrate[J].Journal of Pure and Applied Microbiology, 2018, 12(3):1587-1595.
11. [11]
  阴美晓.枯草芽孢杆菌对金属污染植物厌氧发酵影响的机制研究[D].北京:华北电力大学, 2021.
12. [12]
  HUANG S, LIU D Y, CHEN M Y, et al.Effects of Bacillus subtilis subsp. on the microbial community and aroma components of flue-cured tobacco leaves based on metagenome analysis[J].Archives of Microbiology, 2022, 204(12):726.
13. [13]
  云南省烟草农业科学研究院.枯草芽孢杆菌、红发夫酵母和根霉混合烟草发酵方法:202111038749.1[P].2021-12-28.
14. [14]
  宋丽丽, 张永良, 张志平, 等.白腐菌液体发酵降解烟梗木质素的研究[J].轻工学报, 2019, 34(1):36-42.
15. [15]
  李晓, 顾小燕, 丁美宙, 等.漆酶降解烟梗木质素的条件优化[J].贵州农业科学, 2016, 44(2):149-151.
16. [16]
  SLUITER A, HAMES B, RUIZ R, et al.Determination of structural carbohydrates and lignin in biomass[J]. Laboratory Analytical Procedure, 2008, 1617(1):1-16.
17. [17]
  杨海健, 丁红营, 于国东, 等.咔唑比色法测定造纸法再造烟叶中的果胶含量[J].分析试验室, 2012, 31(6):100-102.
18. [18]
  胡晓龙, 王康丽, 牛广杰, 等.基于高通量测序技术的中温大曲中微生物群落多样性解析[J].轻工学报, 2019, 34(4):21-29.
19. [19]
  YOU L Y, BAO W C, YAO C Q, et al.Changes in chemical composition, structural and functional microbiome during alfalfa (Medicago sativa) ensilage with Lactobacillus plantarum PS-8[J].Animal Nutrition, 2022, 9:100-109.
20. [20]
  郑艳红, 戴芸芸, 杨洋, 等.废次烟叶提取液源木质素降解菌Bacillus subtilis SM降解特性[J].微生物学通报, 2017, 44(7):1525-1534.
21. [21]
  PEREIRA P, ARANTES V, PEREIRA B, et al.Effect of the chemical treatment sequence on pineapple peel fiber:Chemical composition and thermal degradation behavior[J].Cellulose, 2022, 29:8587-8598.
22. [22]
  ZHAO X Y, CHEN J, CHEN F L, et al.Surface characterization of corn stalk superfine powder studied by FTIR and XRD[J].Colloids and Surfaces B(Biointerfaces), 2013, 104:207-212.
23. [23]
  赵一全, 张慧, 张晓昱, 等.木质素的微生物解聚与高值转化[J].微生物学报, 2020, 60(12):2717-2733.
24. [24]
  KHAN M U, AHRING B K.Lignin degradation under anaerobic digestion:Influence of lignin modifications-A review[J].Biomass and Bioenergy, 2019, 128:105325.
25. [25]
  LIANG M, LU W J, LEI P, et al.Physical and combustion properties of binder-assisted hydrochar pellets from hydrothermal carbonization of tobacco stem[J].Waste and Biomass Valorization, 2020, 11(11):6369-6382.
26. [26]
  丁一郎, 叶建斌, 杨金初, 等.生物制备全烟草组分再造烟叶及结构特性分析[J].烟草科技, 2022, 55(7):56-65.
27. [27]
  朱咸鑫, 朱列书, 胡日生, 等.浓香型产区烤烟品种间化学成分和中性致香物质的差异[J].湖南农业大学学报(自然科学版), 2017, 43(5):512-517.
28. [28]
  魏涛, 陈顺心, 黄申, 等.β-胡萝卜素降解菌HC-3发酵条件优化及其对再造烟叶浓缩液增香效果研究[J].轻工学报, 2020, 35(2):24-32.
29. [29]
  陈兴, 申晓峰, 巩效伟, 等.利用微生物制剂提高梗丝品质的研究[J].中国烟草学报, 2013, 19(3):83-86.
30. [30]
  刘文莉, 张娟, 堵国成, 等.一株胶红酵母Rhodotorula mucilaginosa YG14的筛选及混菌发酵提高梗丝品质[J].食品与生物技术学报, 2021, 40(9):64-72.
31. [31]
  孙哲, 黄芷珊, 刘幼强, 等.酒饼叶对豉香型白酒酒饼细菌多样性影响研究[J].食品与发酵工业, 2022, 48(13):63-69.
32. [32]
  潘明凤, 姜曼, 周祚万.木质素生物降解的最新研究进展[J].材料导报, 2011, 25(S2):372-377.
33. [33]
  马英辉, 李利军, 卢美欢, 等.一株伯克霍尔德菌的鉴定及其对木质素的降解特性研究[J].环境污染与防治, 2021, 43(1):25-29.
34. [34]
  肖辰, 陆震鸣, 张晓娟, 等.泸型酒酒醅细菌群落的发酵演替规律[J].微生物学报, 2019, 59(1):195-204.
35. [35]
  赵听, 张凯煜, 谷洁, 等.复合菌群FWD1的木质纤维素降解特性及其微生物多样性研究[J].农业环境科学学报, 2015, 34(8):1582-1588.
Proportional views

Get Citation

PDF

XML

Article Metrics

Article views(5098) PDF downloads(17) Cited by()

Ralated

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Study on degradation characteristics and microbial diversity of tobacco stem by synergistic fermentation with microbial community

Abstract

References

Proportional views

Article Metrics

Ralated

Proportional views

通讯作者: 陈斌, bchen63@163.com