JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 38 Issue 1
February 2023
Article Contents
LI Shitou, PAN Fanda, HUANG Xiaoyu, et al. Screening of tobacco-derived functional microorganisms with different aging time based on metagenomics[J]. Journal of Light Industry, 2023, 38(1): 101-109. doi: 10.12187/2023.01.012
Citation: LI Shitou, PAN Fanda, HUANG Xiaoyu, et al. Screening of tobacco-derived functional microorganisms with different aging time based on metagenomics[J]. Journal of Light Industry, 2023, 38(1): 101-109. doi: 10.12187/2023.01.012 shu

Screening of tobacco-derived functional microorganisms with different aging time based on metagenomics

  • Received Date: 2022-10-08
  • The community structure and functional gene succession of surface microorganisms in Yunnan tobacco leaves at different aging stages were analyzed based on metagenomics. The changes of neutral aroma components during the aging of tobacco leaves were traced. The correlation between microorganisms, enzyme gene and neutral aroma components was explored, and a screening method for tobacco-derived functional microorganisms was established. The results showed at the 3rd, 6th, 9th and 12th month of aging, the dominant genera were Sphingomonas, Enterobacter, Pantoea, and Acinetobacter,respectively. The neutral aroma components in tobacco leaves such as benzyl alcohol, phenylacetaldehyde, 2-acetylpyrrole, solanone, damascenone and megastigmatrienone had an increasing trend. Furfural and β-cyclocitral had a first increase and then decrease trend. The content of hydrocarbons decreased. Klebsiella had a significant positive correlation with β-damascenone, megastrienone and solanone. Acinetobacter had a significant positive correlation with 2-acetylfuran, linalool and megastrienone. Phenylethylaldehyde, benzaldehyde, furfuryl alcohol and cypressene were negatively correlated with almost all carbohydrate synthase, and most glycosidase families were positively correlated with neutral aroma components. A total of 75 strains of microorganisms were screened during tobacco leaf aging, and the microbial types and functions were basically consistent with the results of metagenome analysis, indicating that the analysis results of metagenome could guide microbial screening, enhance the purpose of screening, and improve screening efficiency.
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