中温大曲发酵过程中细菌群落结构解析及功能预测
Bacterial community structure analysis and functional prediction during fermentation of medium-temperature Daqu
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摘要: 利用高通量测序技术解析了中温大曲发酵过程中的细菌群落结构、功能及表型变化。结果表明:中温大曲细菌群落主要由魏斯氏菌属、葡萄球菌属、埃希氏菌属、乳杆菌属等组成;基于OTU水平可将中温大曲样品聚类为前期(0 d和3 d)、中期(9 d和13 d)和后期(30 d)3类;发酵前期以葡萄球菌属为主要优势菌,中期以魏斯氏菌属为主要优势菌,后期则以Muribaculum为主要优势菌;中温大曲细菌菌群的氨基酸转运和代谢、碳水化合物转运和代谢等基因功能丰度较高;与发酵中期相比,发酵前期和后期细菌菌群的细胞壁/膜/包膜生物发生、翻译后修饰、蛋白质折叠和伴侣蛋白等基因功能丰度较高;发酵前期大多为兼性厌氧菌,中期以好氧菌为主,后期则主要是厌氧菌。Abstract: Using high-throughput sequencing technology the changes in bacterial community structure, function and phenotype during the fermentation of medium-temperature Daqu were analyzed. The results showed that the bacterial community of medium-temperature Daqu was mainly composed of Welchiella, Staphylococcus, Escherichia, Lactobacillus, etc. Based on OTU level, the samples of medium-temperature Daqu could be clustered into three stages including early stage (0 d and 3 d), middle stage (9 d and 13 d) and late stage (30 d). Staphylococcus was the main bacteria in the early fermentation stage, weissella was the main dominant bacteria in the middle stage, and muribaculum was the main bacteria in the late fermentation stage. The functional abundance of genes such as amino acid transport and metabolism, carbohydrate transport and metabolism from bacteria inhabiting medium-temperature Daqu was high. The bacterial community in the early and late fermentation stages had higher abundance in gene function such as cell wall/membrane/envelope biogenesis, posttranslational modification, protein turnover, chaperones protein than in the middle fermentation stage. Most microorganisms were facultative anaerobic bacteria in the early stage of fermentation while the aerobic bacteria and anaerobic bacteria dominated in the middle and late stage, respectively.
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-
[1]
WANG B W,WU Q,XU Y,et al.Specific volumetric weight-driven shift in microbiota compositions with saccharifying activity change in starter for Chinese Baijiu fermentation[J].Frontiers in Microbiology,2018,66(22):5425-5432.
-
[2]
梁晨,杜海,徐岩.大曲贮存过程中原核微生物群落结构及风味成分演替规律[J].微生物学通报,2017,44(2):384-393.
-
[3]
JIN G Y,ZHU Y,XU Y.Mystery behind Chinese liquor fermentation[J].Trends in Food Science & Technology,2017,63:18-28.
-
[4]
LIU H L,SUNB G.Effect of fermentation processing on the flavor of Baijiu[J].Journal of Agricultural and Food Chemistry,2018,66(22):5425-5432.
-
[5]
WANG X S,DU H,ZHANG Y,et al.Environmental microbiota drives microbial succession and metabolic profiles during Chinese liquor fermentation[J].Applied & Environmental Microbiology,2017,84(4):02369-17.
-
[6]
WANG C L,SHI D J,GONG G L.Microorganisms in Daqu:A starter culture of Chinese Maotai-flavor liquor[J].World Journal of Microbiology & Biotechnology,2008,24(10):2183-2190.
-
[7]
肖辰.泸型酒中温大曲微生物群落的演替及其对酒醅发酵的贡献[D].无锡:江南大学,2018.
-
[8]
TANG J,CHEN J,CHEN D M,et al.Structural characteristics and formation mechanism of microbiota related to fermentation ability and alcohol production ability in Nongxiang Daqu[J].Foods,2022,11(17):2602.
-
[9]
胡晓龙,王康丽,牛广杰,等.基于高通量测序技术的中温大曲中微生物群落多样性解析[J].轻工学报,2019,34(4):21-29.
-
[10]
吴树坤,谢军,卫春会,等.四川不同地区浓香型大曲微生物群落结构比较[J].食品科学,2019,40(14):144-152.
-
[11]
周天慈,何宏魁,周庆伍,等.基于高通量扩增子测序技术解析中高温大曲微生物来源[J].食品与发酵工业,2021,47(16):66-71.
-
[12]
刘延波,赵志军,陈黄曌,等.高通量测序技术分析浓香型白酒中温曲和高温曲的细菌群落结构[J].现代食品科技,2018,34(5):229-235.
-
[13]
侯强川,王玉荣,王文平,等.茅台和尧治河高温大曲细菌群落结构差异及功能预测[J].食品与发酵工业,2022,48(1):36-44.
-
[14]
刘延波,王一菲,赵志军,等.包包曲与平板曲理化指标及细菌群落结构研究[J].轻工学报,2021,36(6):9-20.
-
[15]
王文平,熊英梅,陈赛浙,等.不同地区米酒曲细菌多样性解析及表型预测[J].食品工业科技,2021,42(20):134-139.
-
[16]
张杰,程伟,李娜,等.夏秋茶大曲与传统中温大曲中微生物群落及多样性研究[J].酿酒科技,2021(11):36-44.
-
[17]
ZHU M,ZHENG J,XIE J,et al.Effects of environmental factors on the microbial community changes during medium-high temperature Daqu manufacturin[J].Food Research International,2022,153:110955.
-
[18]
宁亚丽,吴跃,何嫱,等.基于高通量测序技术分析朝鲜族传统米酒及其酒曲中微生物群落多样性[J].食品科学,2019,40(16):107-114.
-
[19]
KE L Q,WANG L L,LI H B,et al.Molecular identification of lactic acid bacteria in Chinese rice wine using species-specific multiplex PCR[J].European Food Research and Technology,2014,239(1):59-65.
-
[20]
陈蒙恩,侯建光,张振科,等.陶融型大曲微生物多样性与挥发性风味成分的相关性研究[J].中国酿造,2020,39(10):54-60.
-
[21]
HUANG Y H,YI Z L,JIN Y L,et al.New microbial resource: microbial diversity,function and dynamics in Chinese liquor starter[J].Scientific Reports,2017,7:14577.
-
[22]
胡小霞,黄永光,涂华彬,等.酱香型白酒1轮次酿造细菌的菌群结构[J].食品科学,2020,41(14):175-182.
-
[23]
郑亚伦,赵婷,王家胜,等.数字化高温大曲发酵过程中微生物群落结构的变化[J].食品科学,2022,43(12):171-178.
-
[24]
王欢.基于微生物组学的酱香型白酒酿造微生物及酒体风味研究[D].贵阳:贵州大学,2019.
-
[25]
邢敏钰,杜海,徐岩.芝麻香型白酒发酵过程中乳酸菌多样性及其演替规律[J].微生物学通报,2018,45(1):19-28.
-
[26]
WANG X S,DU H,XU Y.Source tracking of prokaryotic communities in fermented grain of Chinese strong-flavor liquor[J].International Journal of Food Microbiology,2017,244:27-35.
-
[27]
YAN S B,CHEN X S,GUANG J Q.Bacterial and fungal diversity in the traditional Chinese strong flavour liquor Daqu[J].Journal of the Institute of Brewing,2019,125(4):443-452.
-
[28]
ZHENG X W,YAN Z,NOUT M J R,et al.Characterization of the microbial community in different types of Daqu samples as revealed by 16S rRNA and 26S rRNA gene clone libraries[J].World Journal of Microbiology and Biotechnology,2015,31(1):199-208.
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