JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

基于改进G-四链体DNA酶的电化学适配体传感器构建及卡那霉素高灵敏检测

伍永梅 ,  朱肖倩 ,  方娇 ,  白艳红

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伍永梅, 朱肖倩, 方娇, 等. 基于改进G-四链体DNA酶的电化学适配体传感器构建及卡那霉素高灵敏检测[J]. 轻工学报, 2023, 38(6): 62-69. doi: 10.12187/2023.06.008
引用本文: 伍永梅, 朱肖倩, 方娇, 等. 基于改进G-四链体DNA酶的电化学适配体传感器构建及卡那霉素高灵敏检测[J]. 轻工学报, 2023, 38(6): 62-69. doi: 10.12187/2023.06.008
shu
WU Yongmei, ZHU Xiaoqian, FANG Jiao and et al. Construction of electrochemical aptasensor for highly sensitive detection of kanamycin based on the improved G-quadruplex DNAzyme[J]. Journal of Light Industry, 2023, 38(6): 62-69. doi: 10.12187/2023.06.008
Citation: WU Yongmei, ZHU Xiaoqian, FANG Jiao and et al. Construction of electrochemical aptasensor for highly sensitive detection of kanamycin based on the improved G-quadruplex DNAzyme[J]. Journal of Light Industry, 2023, 38(6): 62-69. doi: 10.12187/2023.06.008
shu

基于改进G-四链体DNA酶的电化学适配体传感器构建及卡那霉素高灵敏检测

  • 1. 郑州轻工业大学 食品与生物工程学院, 河南 郑州 450001;

  • 2. 河南省冷链食品质量安全控制重点实验室, 河南 郑州 450001;

  • 3. 食品生产与安全河南省协同创新中心, 河南 郑州 450001

    • 作者简介: 伍永梅(1987-),女,河南省新乡市人,郑州轻工业大学讲师,博士,主要研究方向为食品安全和疾病标志物检测。E-mail:wuyongmei@zzuli.edu.cn;

  • 基金项目: 河南省重点研发与推广专项项目（222102310140）；河南省高等学校重点科研项目（21A550015）；郑州轻工业大学博士启动科研基金项目（0123-13501050070）

  • 中图分类号: TS207.3

Construction of electrochemical aptasensor for highly sensitive detection of kanamycin based on the improved G-quadruplex DNAzyme

  • 1. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;

  • 2. Henan Key Laboratory of Cold Chain Food Quality and Safety Control, Zhengzhou 450001, China;

  • 3. Collaborative Innovation Center of Food Production and Safety of Henan Province, Zhengzhou 450001, China

  • Received Date: 2023-03-16
    Accepted Date: 2023-05-01

    CLC number: TS207.3

  • 摘要: 将阳离子多肽（peptide）与G-四链体DNA酶（G4 DNAzyme）共价组装得到高活性的G4 DNAzyme-peptide复合物，进一步构建新型电化学适配体传感器，研究该复合物对电化学适配体传感器响应性能的影响，并将该传感器用于牛奶中卡那霉素（KANA）的检测分析。结果表明：G4 DNAzyme-peptide可以显著放大电化学信号，明显提高传感器检测灵敏度；在捕获探针序列最优浓度（2.0 μmol/L）下，电流信号强度变化与目标物浓度（0.06 pmol/L~20 nmol/L）对数值呈良好的线性关系，检测限为0.02 pmol/L，优于其他KANA检测方法；该传感器对KANA具有良好的选择性，在实际牛奶样品检测中，KANA的加标回收率为97.1%~105.5%，相对标准差为3.60%~5.74%，且检测结果与ELISA法一致，说明该传感器能够实现牛奶中KANA的高灵敏检测。
    Abstract: A highly active G4 DNAzyme-peptide complex obtained by covalent assembly of cationic peptide on G4 DNAzyme was used to construct a novel electrochemical aptasensor. The effect of complex on the performance of electrochemical aptasensor was investigated and the proposed aptasensor was used to detect kanamycin (KANA) in milk. The experimental results indicated that G4 DNAzyme-peptide could significantly amplify the electrochemical signal and greatly improve the detection sensitivity of aptasensor. Under the optimal concentration of capture probe (CP, 2.0 μmol/L), the change of current intensity showed a good linear relationship with the logarithm of the target concentration in the range of 0.06 pmol/L~20 nmol/L, and the detection limit was 0.02 pmol/L, which was superior to other KANA detection methods. The aptasensor showed better selectivity and lower detection limit and the recoveries obtained in milk sample were from 97.1% to 105.5% and the relative standard deviation were from 3.60% to 5.74%. The experimental results were consistent with ELISA method, indicating the proposed aptasensor could achieve high sensitivity for the detection of KANA in milk.
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伍永梅, 朱肖倩, 方娇, 等. 基于改进G-四链体DNA酶的电化学适配体传感器构建及卡那霉素高灵敏检测[J]. 轻工学报, 2023, 38(6): 62-69. doi: 10.12187/2023.06.008
引用本文: 伍永梅, 朱肖倩, 方娇, 等. 基于改进G-四链体DNA酶的电化学适配体传感器构建及卡那霉素高灵敏检测[J]. 轻工学报, 2023, 38(6): 62-69. doi: 10.12187/2023.06.008
shu
WU Yongmei, ZHU Xiaoqian, FANG Jiao and et al. Construction of electrochemical aptasensor for highly sensitive detection of kanamycin based on the improved G-quadruplex DNAzyme[J]. Journal of Light Industry, 2023, 38(6): 62-69. doi: 10.12187/2023.06.008
Citation: WU Yongmei, ZHU Xiaoqian, FANG Jiao and et al. Construction of electrochemical aptasensor for highly sensitive detection of kanamycin based on the improved G-quadruplex DNAzyme[J]. Journal of Light Industry, 2023, 38(6): 62-69. doi: 10.12187/2023.06.008
shu

基于改进G-四链体DNA酶的电化学适配体传感器构建及卡那霉素高灵敏检测

    作者简介:伍永梅(1987-),女,河南省新乡市人,郑州轻工业大学讲师,博士,主要研究方向为食品安全和疾病标志物检测。E-mail:wuyongmei@zzuli.edu.cn
  • 1. 郑州轻工业大学 食品与生物工程学院, 河南 郑州 450001;
  • 2. 河南省冷链食品质量安全控制重点实验室, 河南 郑州 450001;
  • 3. 食品生产与安全河南省协同创新中心, 河南 郑州 450001
  • 收稿日期: 2023-03-16
  • 录用日期: 2023-05-01
基金项目:  河南省重点研发与推广专项项目（222102310140）；河南省高等学校重点科研项目（21A550015）；郑州轻工业大学博士启动科研基金项目（0123-13501050070）

摘要: 将阳离子多肽（peptide）与G-四链体DNA酶（G4 DNAzyme）共价组装得到高活性的G4 DNAzyme-peptide复合物，进一步构建新型电化学适配体传感器，研究该复合物对电化学适配体传感器响应性能的影响，并将该传感器用于牛奶中卡那霉素（KANA）的检测分析。结果表明：G4 DNAzyme-peptide可以显著放大电化学信号，明显提高传感器检测灵敏度；在捕获探针序列最优浓度（2.0 μmol/L）下，电流信号强度变化与目标物浓度（0.06 pmol/L~20 nmol/L）对数值呈良好的线性关系，检测限为0.02 pmol/L，优于其他KANA检测方法；该传感器对KANA具有良好的选择性，在实际牛奶样品检测中，KANA的加标回收率为97.1%~105.5%，相对标准差为3.60%~5.74%，且检测结果与ELISA法一致，说明该传感器能够实现牛奶中KANA的高灵敏检测。

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