基于离子液体的菌藻类胡萝卜素提取工艺研究

张志平; 宋洋洋; 王秋领; 王清福; 龚贵平; 付瑜锋; 陈高

doi:10.12187/2024.02.003

April 2024

Article Contents

ZHANG Zhiping, SONG Yangyang, WANG Qiuling, et al. Research on extraction process of carotenoids from yeasts and algae based on ionic liquid[J]. Journal of Light Industry, 2024, 39(2): 19-27. doi: 10.12187/2024.02.003

Citation: ZHANG Zhiping, SONG Yangyang, WANG Qiuling, et al. Research on extraction process of carotenoids from yeasts and algae based on ionic liquid[J]. Journal of Light Industry, 2024, 39(2): 19-27. doi: 10.12187/2024.02.003 shu

Research on extraction process of carotenoids from yeasts and algae based on ionic liquid

1. College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
2. Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou 450003, China;
3. Biogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu 610042, China;
4. Shandong Academy of Agricultural Science, Jinan 250100, China

Received Date: 2023-10-24
Accepted Date: 2024-02-24

Abstract

Using Rhodotorula glutinis and Chlorella vularis sludge as raw material, the extraction process of carotenoids from yeast and algae was optimized through single factor and response experiments, and the in vitro antioxidant activity of carotenoids from yeast and algae was investigated. The results showed that the optimal ionic liquid for yeast and algal wall breaking was 1-butyl-3-methylimidazole chloride (BMIMCl), and the optimal extraction process for carotene from yeast and algal sludge was 1 mol/L ionic liquid concentration, pre-treatment temperature of 53 ℃, and pre-treatment time of 57 min. Under this condition, the extraction amount of carotene from yeast and algae was 1.311 mg/g. When the mass concentration was 30 μg/mL, the scavenging rates of DPPH free radical, ·OH free radical and ABTS⁺ free radical were 82.3 %, 37.6 % and 86.8 %, respectively, and the reducing power was 0.289, showing good antioxidant activity. The yeast and algal sludge treated with BMIMCl had a higher content of extracted carotenoids and better antioxidant activity.
- ionic liquid,
- yeast and algal sludge,
- carotenoids,
- extraction process,
- antioxidant activity
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Research on extraction process of carotenoids from yeasts and algae based on ionic liquid

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