烟草废弃物提取物的双水相萃取及其化妆品功效研究

沙云菲; 熊骏威; 费婷; 吴达; 任爽; 蒋汶

doi:10.12187/2024.02.010

CN 41-1437/TS ISSN 2096-1553

沙云菲 , 熊骏威 , 费婷 , 吴达 , 任爽 , 蒋汶

沙云菲, 熊骏威, 费婷, 等. 烟草废弃物提取物的双水相萃取及其化妆品功效研究[J]. 轻工学报, 2024, 39(2): 80-86,93. doi: 10.12187/2024.02.010

引用本文: 沙云菲, 熊骏威, 费婷, 等. 烟草废弃物提取物的双水相萃取及其化妆品功效研究[J]. 轻工学报, 2024, 39(2): 80-86,93. doi: 10.12187/2024.02.010

SHA Yunfei, XIONG Junwei, FEI Ting, et al. Two-phase extraction of tobacco waste extracts and their cosmetic efficacy[J]. Journal of Light Industry, 2024, 39(2): 80-86,93. doi: 10.12187/2024.02.010

Citation: SHA Yunfei, XIONG Junwei, FEI Ting, et al. Two-phase extraction of tobacco waste extracts and their cosmetic efficacy[J]. Journal of Light Industry, 2024, 39(2): 80-86,93. doi: 10.12187/2024.02.010

烟草废弃物提取物的双水相萃取及其化妆品功效研究

1. 上海烟草集团有限责任公司, 上海 200082;
2. 上海应用技术大学香料香精技术与工程学院, 上海 201418;
3. 香料香精化妆品省部共建协同创新中心, 上海 201418

作者简介: 沙云菲(1980-),女,浙江省鄞县人,上海烟草集团有限责任公司高级工程师,主要研究方向为烟草化学分析与应用技术。E-mail:shayf@sh.tobacco.com.cn;

基金项目: 香料香精化妆品省部共建协同创新中心开放课题项目(XTCXC-202101)
上海应用技术大学引进人才科研启动项目(YJ2021-89)
上海市科委地方能力建设项目(19090503600)
上海高校青年教师培养资助计划项目(ZZ202212008)
中图分类号: TS49

Two-phase extraction of tobacco waste extracts and their cosmetic efficacy

1. Shanghai Tobacco Group Co., Ltd., Shanghai 200082, China;
2. School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China;
3. Collaborative Innovation Center of Fragrance Flavour and Cosmetics, Shanghai 201418, China
Received Date: 2023-03-08
Accepted Date: 2023-07-12

CLC number: TS49

摘要: 采用双水相萃取法制备烟草废弃物提取物,探究不同萃取条件对其中上相多酚得率的影响,并探讨其抗氧化活性、紫外吸收特性和细胞毒性。结果表明:当料液质量比为1:60,搅拌时间为30 min,(NH₄)₂SO₄质量分数为23.3 %,乙醇质量分数为23.5 %时,上相多酚得率最高,为48.59 mg/g;该萃取条件下,烟草废弃物提取物具有较好的抗氧化活性,当质量浓度为2 mg/mL时,对DPPH自由基清除率达98 %,对ABTS自由基清除率达78 %;当提取物质量浓度为0.5 mg/mL时,具有较好的紫外吸收特性,有一定的光防护功效;当提取物质量浓度在0~1.2 mg/mL范围内时,对Hacat细胞无细胞毒性,有望成为天然无刺激的功效型化妆品原料。
- 烟草废弃物 /
- 多酚 /
- 双水相萃取法 /
- 化妆品 /
- 紫外吸收特性 /
- 抗氧化活性
Abstract: A two-phase aqueous extraction method was established to prepare tobacco waste extracts. The effects of different extraction conditions on the yield of upper phase polyphe nols were studied. The antioxidant activity and UV absorption chaoracteristic were also discussed. The results indicated when the solid-liquid ratio of tobacco waste was 1:60, the stirring time was 30 min, the mass fraction of (NH₄)₂SO₄ was 23.3 % and the ethanol was 23.5 %, the extraction rate of upper phase polyphenols was the highest, and the content of polyphenol was 48.59 mg/g. The tobacco waste extracts showed good antioxidant activity, and the scavenging rate of DPPH free radical was 98 % and ABTS free radical was 78 % at the mass concentration of 2 mg/mL. When the mass concentration of the extracts was 0.5 mg/mL, the extracts had good UV absorption and a certain effect of light protection. When the mass concentration of the extracts was within the range of 0~1. 2 mg/mL, the extracts had no cytotoxicity to HaCat cells and were expected to become a natural and non-irritating functional cosmetic raw material.
- tobacco waste extract /
- polyphenol /
- dual aqueous phase extraction method /
- cosmetics /
- UV absorption characteristic /
- antioxidant activity
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沙云菲, 熊骏威, 费婷, 等. 烟草废弃物提取物的双水相萃取及其化妆品功效研究[J]. 轻工学报, 2024, 39(2): 80-86,93. doi: 10.12187/2024.02.010

引用本文: 沙云菲, 熊骏威, 费婷, 等. 烟草废弃物提取物的双水相萃取及其化妆品功效研究[J]. 轻工学报, 2024, 39(2): 80-86,93. doi: 10.12187/2024.02.010

SHA Yunfei, XIONG Junwei, FEI Ting, et al. Two-phase extraction of tobacco waste extracts and their cosmetic efficacy[J]. Journal of Light Industry, 2024, 39(2): 80-86,93. doi: 10.12187/2024.02.010

烟草废弃物提取物的双水相萃取及其化妆品功效研究

作者简介:沙云菲(1980-),女,浙江省鄞县人,上海烟草集团有限责任公司高级工程师,主要研究方向为烟草化学分析与应用技术。E-mail:shayf@sh.tobacco.com.cn

1. 上海烟草集团有限责任公司, 上海 200082;
2. 上海应用技术大学香料香精技术与工程学院, 上海 201418;
3. 香料香精化妆品省部共建协同创新中心, 上海 201418

收稿日期: 2023-03-08
录用日期: 2023-07-12

基金项目: 香料香精化妆品省部共建协同创新中心开放课题项目(XTCXC-202101)上海应用技术大学引进人才科研启动项目(YJ2021-89)上海市科委地方能力建设项目(19090503600)上海高校青年教师培养资助计划项目(ZZ202212008)

关键词:

摘要: 采用双水相萃取法制备烟草废弃物提取物,探究不同萃取条件对其中上相多酚得率的影响,并探讨其抗氧化活性、紫外吸收特性和细胞毒性。结果表明:当料液质量比为1:60,搅拌时间为30 min,(NH₄)₂SO₄质量分数为23.3 %,乙醇质量分数为23.5 %时,上相多酚得率最高,为48.59 mg/g;该萃取条件下,烟草废弃物提取物具有较好的抗氧化活性,当质量浓度为2 mg/mL时,对DPPH自由基清除率达98 %,对ABTS自由基清除率达78 %;当提取物质量浓度为0.5 mg/mL时,具有较好的紫外吸收特性,有一定的光防护功效;当提取物质量浓度在0~1.2 mg/mL范围内时,对Hacat细胞无细胞毒性,有望成为天然无刺激的功效型化妆品原料。

English Abstract

Two-phase extraction of tobacco waste extracts and their cosmetic efficacy

1. Shanghai Tobacco Group Co., Ltd., Shanghai 200082, China;
2. School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China;
3. Collaborative Innovation Center of Fragrance Flavour and Cosmetics, Shanghai 201418, China

Received Date: 2023-03-08
Accepted Date: 2023-07-12

Abstract: A two-phase aqueous extraction method was established to prepare tobacco waste extracts. The effects of different extraction conditions on the yield of upper phase polyphe nols were studied. The antioxidant activity and UV absorption chaoracteristic were also discussed. The results indicated when the solid-liquid ratio of tobacco waste was 1:60, the stirring time was 30 min, the mass fraction of (NH₄)₂SO₄ was 23.3 % and the ethanol was 23.5 %, the extraction rate of upper phase polyphenols was the highest, and the content of polyphenol was 48.59 mg/g. The tobacco waste extracts showed good antioxidant activity, and the scavenging rate of DPPH free radical was 98 % and ABTS free radical was 78 % at the mass concentration of 2 mg/mL. When the mass concentration of the extracts was 0.5 mg/mL, the extracts had good UV absorption and a certain effect of light protection. When the mass concentration of the extracts was within the range of 0~1. 2 mg/mL, the extracts had no cytotoxicity to HaCat cells and were expected to become a natural and non-irritating functional cosmetic raw material.