基于随机蛙跳筛选的初烤烟叶中<i>β</i>-胡萝卜素和叶黄素含量近红外模型的建立

何屹; 杨本刚; 尹嵩; 陈实; 尹晓东; 周文忠; 贾学伟; 杨盼盼

doi:10.12187/2024.02.013

April 2024

Article Contents

HE Yi, YANG Bengang, YIN Song, et al. Establishment of near infrared models of flue-cured tobacco leaves β-carotene and lutein based on random frog screening[J]. Journal of Light Industry, 2024, 39(2): 100-106. doi: 10.12187/2024.02.013

Citation: HE Yi, YANG Bengang, YIN Song, et al. Establishment of near infrared models of flue-cured tobacco leaves β-carotene and lutein based on random frog screening[J]. Journal of Light Industry, 2024, 39(2): 100-106. doi: 10.12187/2024.02.013 shu

Establishment of near infrared models of flue-cured tobacco leaves β-carotene and lutein based on random frog screening

HE Yi ¹ ,
YANG Bengang ¹ ,
YIN Song ¹ ,
CHEN Shi ² ,
YIN Xiaodong ³ ,
ZHOU Wenzhong ⁴ ,
JIA Xuewei ⁵ ,
YANG Panpan ⁴

1. Sichuan Sanlian New Materials Co., Ltd., Chengdu 610101, China;
2. Bijie Cigarette Factory, Guizhou Zhongyan Industrial Co., Ltd., Bijie 551799, China;
3. Yunnan Tobacco Leaf Company, Kunming 652017, China;
4. Yunnan Tongchuang Testing Technology Co., Ltd., Kunming 650106, China;
5. College of Tobacco Science and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China

Received Date: 2023-05-09
Accepted Date: 2023-08-23

Abstract

To improve the accuracy and stability of rapid determination of β-carotene and lutein in flue-cured by near infrared spectroscopy, this study used random frog to screen the characteristic wavelength of partial least squares (PLS) near infrared model of the above indicators. Furthermore, the optimal number of wavelengths and principal components were determined by internal interaction verification, and the accuracy and stability of the optimal model were verified by internal parameters and external parameters. The results showed that:compared with the full-wavelength(1298 wavelengths) model, the determination coefficient R² of the β-carotene and lutein models established 181 and 216 wavelength variables by RF wavelength screening combined with PLS was increased from 0.771 8 and 0.867 7 to 0.951 1 and 0.958 7, respectively. The corrected values of root mean square error of calibration (RMSEC) was reduced from 3.362 μg/g and 4.930 μg/g to 1.556 μg/g and 2.756 μg/g, respectively. The values of root mean square error of cross validation (RMSECV) was reduced from 3.304 μg/g, 4.836 μg/g to 1.529 μg/g, 2.703 μg/g; The average relative error of external validation (50 samples) decreased from 11.80 % and 9.62 % to 5.48 % and 5.49 %, respectively. The random frog screening wavelength method could significantly improve the accuracy and stability of the near infrared models for the determination of β-carotene and lutein of flue-cured tobacco, which could provide more accurate and reliable data reference for its quality evaluation.
- near infrared model,
- random frog,
- flue-cured tobacco leaf,
- β-carotene,
- lutein
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Establishment of near infrared models of flue-cured tobacco leaves β-carotene and lutein based on random frog screening

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