基于低场核磁共振的烤烟烟叶石油醚提取物含量检测方法

付光明; 韦凤杰; 常剑波; 贾保顺; 李致新; 来苗; 杨晓朋; 姬小明

doi:10.12187/2024.04.011

August 2024

Article Contents

FU Guangming, WEI Fengjie, CHANG Jianbo, et al. Determination of petroleum ether extracts in flue-cured tobacco leaves by low-field nuclear magnetic resonance[J]. Journal of Light Industry, 2024, 39(4): 80-88. doi: 10.12187/2024.04.011

Citation: FU Guangming, WEI Fengjie, CHANG Jianbo, et al. Determination of petroleum ether extracts in flue-cured tobacco leaves by low-field nuclear magnetic resonance[J]. Journal of Light Industry, 2024, 39(4): 80-88. doi: 10.12187/2024.04.011 shu

Determination of petroleum ether extracts in flue-cured tobacco leaves by low-field nuclear magnetic resonance

1. Tobacco College, Henan Agricultural University, Zhengzhou 450046, China;
2. Henan Tobacco Company, Zhengzhou 450018, China;
3. Henan Tobacco Company Sanmenxia City Company, Sanmenxia 472000, China

Received Date: 2023-08-01
Accepted Date: 2023-10-25
Available Online: 2024-08-15

Abstract

In response to the problems of slow detection speed, cumbersome operation, and solvent pollution in the detection of petroleum ether extract in tobacco leaves using the current traditional Soxhlet extraction method, low field nuclear magnetic resonance technology was used to detect the petroleum ether extract content in tobacco leaves. The detection parameters such as drying time, sample quality, echo time, and number of repeated scans were optimized to verify the repeatability and accuracy of the method, and the detection results were compared with the traditional Soxhlet extraction method. The results showed that 3.0 g of tobacco leaf powder passing through a 40 mesh sieve was dried at 80 ℃ for 4 hours. Under the conditions of echo time of 0.25 ms and 8 repeated scans, the petroleum ether extract detection results were good. The standard curve R² reached 0.999 81, and the relative standard deviation of repeatability was 1.45% to 2.99%. The intra day and inter day precision were 2.61% and 1.10%, respectively. The correlation coefficient between the detection results of this method and the Soxhlet extraction method reaches 0.998 55, with an absolute error within 0.02%~0.35% and a relative error within 0.26%~3.69%. Compared with the traditional Soxhlet extraction method, this method is simple, fast, accurate, and solvent free, making it suitable for the accurate quantification of petroleum ether extracts from large quantities of flue-cered tobacco leaves.
- low-field nuclear magnetic resonance,
- petroleum ether extract,
- tobacco leaf powder,
- repeatability,
- precision
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Determination of petroleum ether extracts in flue-cured tobacco leaves by low-field nuclear magnetic resonance

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