JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

苦荞麦麸碱提多糖的制备与分析

孙元琳 ,  陕方 ,  边俊生 ,  白洁

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孙元琳, 陕方, 边俊生, 等. 苦荞麦麸碱提多糖的制备与分析[J]. 轻工学报, 2011, 26(2): 1-4. doi: 10.3969/j.issn.1004-1478.2011.02.001
引用本文: 孙元琳, 陕方, 边俊生, 等. 苦荞麦麸碱提多糖的制备与分析[J]. 轻工学报, 2011, 26(2): 1-4. doi: 10.3969/j.issn.1004-1478.2011.02.001
shu
SUN Yuan-lin, SHAN Fang, BIAN Jun-sheng and et al. Preparation and analysis of alkali-soluble polysaccharide from tartary buckwheat bran[J]. Journal of Light Industry, 2011, 26(2): 1-4. doi: 10.3969/j.issn.1004-1478.2011.02.001
Citation: SUN Yuan-lin, SHAN Fang, BIAN Jun-sheng and et al. Preparation and analysis of alkali-soluble polysaccharide from tartary buckwheat bran[J]. Journal of Light Industry, 2011, 26(2): 1-4. doi: 10.3969/j.issn.1004-1478.2011.02.001
shu

苦荞麦麸碱提多糖的制备与分析

  • 运城学院 生命科学系, 山西 运城 044000;

  • 山西省农科院 农产品综合利用研究所, 山西 太原 030031

  • 基金项目: "十一五"国家科技支撑计划子课题项目(2006BAD02B06-19)

  • 中图分类号: S38

Preparation and analysis of alkali-soluble polysaccharide from tartary buckwheat bran

  • Dept.of Life Sci., Yuncheng Univ., Yuncheng 044000, China;

  • Inst.of Farm Products Comprehensive Utilization, Shanxi Academy of Agr.Sci., Taiyuan 030031, China

  • Received Date: 2010-12-28
    Available Online: 2011-03-15

    CLC number: S38

  • 摘要: 以苦荞麦麸为原料、0.2 mol/L NaOH溶液为提取介质分离碱提多糖,并采用DEAE-Sephrose CL-6B柱层析技术进行分级纯化.结果表明:苦荞麦麸碱提多糖(ABP)主要由葡萄糖(Glc)组成,并含有少量阿拉伯糖(Ara)、木糖(Xyl)、半乳糖(Gal)和微量甘露糖(Man),初步推测ABP主要为β-葡聚糖.ABP经离子交换柱层析分级为2个组分:ABP1和ABP2,ABP1的多糖含量(85.30%)较ABP(64.53%)有所提高,且只含有Glc和少量Xyl,其中n(Glc):n(Xyl)=9.47:1.ABP1经凝胶色谱鉴定为均-组分,其相对分子质量为8.5×104.
    Abstract: With tartary buckwheat bran as material,alkali-soluble polysaccharide was extracted with 0.2 mol/L NaOH solution and purified on DEAE-Sepharose CL-6B chromatography.The results showed that ABP was mainly composed of glucose,along with small amount of arabinose,xylose,and mannose,which was mainly β-gulcan.ABP was fractionated into 2 fractions on DEAE-Sepharose CL-6B column:ABP1 and ABP2.ABP1,with 85.30% of polysaccharide,consisted mainly of glucose,and small amount of xylose.The molar ratio was n(Glc):n(Xyl)=9.47:1.The molecular weight was 8.5×104 determined by high-performance size-exclusion chromatography.
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  • 收稿日期:  2010-12-28
  • 刊出日期:  2011-03-15
通讯作者: 陈斌, bchen63@163.com
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孙元琳, 陕方, 边俊生, 等. 苦荞麦麸碱提多糖的制备与分析[J]. 轻工学报, 2011, 26(2): 1-4. doi: 10.3969/j.issn.1004-1478.2011.02.001
引用本文: 孙元琳, 陕方, 边俊生, 等. 苦荞麦麸碱提多糖的制备与分析[J]. 轻工学报, 2011, 26(2): 1-4. doi: 10.3969/j.issn.1004-1478.2011.02.001
shu
SUN Yuan-lin, SHAN Fang, BIAN Jun-sheng and et al. Preparation and analysis of alkali-soluble polysaccharide from tartary buckwheat bran[J]. Journal of Light Industry, 2011, 26(2): 1-4. doi: 10.3969/j.issn.1004-1478.2011.02.001
Citation: SUN Yuan-lin, SHAN Fang, BIAN Jun-sheng and et al. Preparation and analysis of alkali-soluble polysaccharide from tartary buckwheat bran[J]. Journal of Light Industry, 2011, 26(2): 1-4. doi: 10.3969/j.issn.1004-1478.2011.02.001
shu

苦荞麦麸碱提多糖的制备与分析

  • 运城学院 生命科学系, 山西 运城 044000;
  • 山西省农科院 农产品综合利用研究所, 山西 太原 030031
  • 收稿日期: 2010-12-28
  • 网络出版日期: 2011-03-15
基金项目:  "十一五"国家科技支撑计划子课题项目(2006BAD02B06-19)

摘要: 以苦荞麦麸为原料、0.2 mol/L NaOH溶液为提取介质分离碱提多糖,并采用DEAE-Sephrose CL-6B柱层析技术进行分级纯化.结果表明:苦荞麦麸碱提多糖(ABP)主要由葡萄糖(Glc)组成,并含有少量阿拉伯糖(Ara)、木糖(Xyl)、半乳糖(Gal)和微量甘露糖(Man),初步推测ABP主要为β-葡聚糖.ABP经离子交换柱层析分级为2个组分:ABP1和ABP2,ABP1的多糖含量(85.30%)较ABP(64.53%)有所提高,且只含有Glc和少量Xyl,其中n(Glc):n(Xyl)=9.47:1.ABP1经凝胶色谱鉴定为均-组分,其相对分子质量为8.5×104.

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