Abstract: A realization method of two-machine serial communication based on LabVIEW was presented,using serial line to design the communication between two computers,and a communications system which can automatically send and receive had been realized through LabVIEW's VISA library.The serial communication control module was built up in a logical sequence,at the same time,looping statements,condition judgment statements and other statements were used and the data transfer format or instrument control command is converted to LabVIEW platform.The method is practical with a simple hardware interface,convenient software programming and practice.It has some reference value in the actual close communication.