不同燕麦产品对正常小鼠消化酶及Na+-K+-ATP酶活性的影响
Effect of different oat products on digestive enzyme and Na+-K+-ATPase activity of normal mice
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摘要: 探讨了不同的燕麦产品对正常小鼠胃肠道消化酶和小肠黏膜Na+-K+-ATP酶活性的影响.将实验动物随机分为空白对照和分别饲喂燕麦面粉、燕麦麸皮、燕麦片、燕麦β-葡聚糖计5组,饲喂3周,测定血清胰蛋白酶、脂肪酶、淀粉酶以及小肠黏膜Na+-K+-ATP酶活性,并测体重、进食量等指标.结果显示:饲喂3周后,面粉组小鼠体重和进食量与对照组基本一致,其他实验组小鼠体重下降,进食量减少,与对照组相比差异显著(P<0.05).燕麦产品对小鼠消化酶活性的抑制作用也很明显,除面粉组外,各实验组血清淀粉酶活性与对照组相比均差异显著(P<0.05);各实验组胰蛋白酶、脂肪酶和Na+-K+-ATP酶活性与对照组相比显著降低(P<0.05).实验表明:燕麦产品可抑制小鼠消化酶和小肠黏膜Na+-K+-ATP酶的活性,降低营养物质的利用率,降低体重,减少进食量.其变化过程与不同燕麦产品中β-葡聚糖含量呈正相关.
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关键词:
- 燕麦产品 /
- 消化酶 /
- Na+-K+-ATP酶
Abstract: The effect of different oat products on digestive enzyme and Na+-K+-ATPase activity of normal mice was studied.The experimental animals were randomly divided into control group,oat β-glucan group,oat flour group,oat meal group and oat bran group.The mice were fed for three weeks.The intestinal mucosa Na+-K+-ATPase activities and digestive enzyme were determined by spectrophotometry.The body weight and food in-take were also examined.The results showed that after three weeks, the mice body weight and food in-take of oat flour group is fit to control group,other groups body weight and food in-take of mice decrease,and there is significant difference comparing with control group(P<0.05).Except for the oat flour group,the others suppressed body weight gain of mice and resulted in significantly decreased food in-take and amylse activity compared with the control group(P<0.05);the trypsin,lipase and Na+-K+-ATPase activity of all experimental groups were significantly lower than the control group(P<0.05).The result showed oat products could decrease level of body weight,food in-take,and intestinal mucosa Na+-K+-ATPase and digestive enzyme activity of mice.The availability of nutrient was depression.The change course is positive correlation with β-glucan of different oat products.-
Key words:
- oat products /
- digestive enzyme /
- Na+-K+-ATPase
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