石斛多糖超高压提取工艺条件的优化
Technique condition optimization for ultra high pressure extraction of dendrobium polysaccharide
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摘要: 采用超高压方法提取铁皮石斛中的多糖成分.在单因素实验的基础上,选取压力、时间、粉碎度和固液比(g:mL)4个影响因素,以石斛多糖为指标,通过正交试验优化超高压方法提取石斛多糖的工艺条件.结果表明,将石斛粉碎到80目后,按固液比1:20,采用300 MPa的压力提取6min后,石斛多糖的得率达到19.27%.这表明超高压方法是一有效的提取石斛多糖的方法.Abstract: Dendrobium candidum polysaccharide was extracted by ultra high pressure extraction (UHPE) method.Based on single factor experiments,orthogonal test was used to optimize the extraction conditions.Pressure,holding time,size and ratio of solid to liquid (g:mL) were as 4 factors and the yield of dendrobium polysaccharide (DP) was as target.The optimum parameters for extracting DP were as follows:pressure 300 MPa,holding time 6 min,size 80 mesh and ratio of solid to liquid (g:mL) 1:20.The yield of DP was 19.27%.So UHPE was a suitable method for extraction of DP.
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