XU Chun-ping, SHI Chao-wen and MAO Duo-bin. Analysis of exopolysaccharides from fermented Pleurotus pulmonarius and its application in cigarette[J]. Journal of Light Industry, 2013, 28(1): 41-44. doi: 10.3969/j.issn.2095-476X.2013.01.010
Citation:
XU Chun-ping, SHI Chao-wen and MAO Duo-bin. Analysis of exopolysaccharides from fermented Pleurotus pulmonarius and its application in cigarette[J]. Journal of Light Industry, 2013, 28(1): 41-44.
doi:
10.3969/j.issn.2095-476X.2013.01.010
Analysis of exopolysaccharides from fermented Pleurotus pulmonarius and its application in cigarette
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College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
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College of Life Science, He'nan Agriculture University, Zhengzhou 450002, China
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Received Date:
2012-09-07
Available Online:
2013-01-15
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Abstract
Pleurotus pulmonarius was cultivated in a 5 L stirred-tank reactor,and exopolysaccharides(EPS) were extracted from fermentation broth.After EPS was deproteinized by Sevag method,one group of EPS was obtained from the culture filtrates by gel filtration chromatography on Sepharose CL-6B.FT-IR spectroscopy was used for obtaining vibrational spectra of the purified EPS fraction.Polysaccharide is acidic polysaccharides,mainly consist of glucose and mannose.Different concentrations of EPS from Coriolus versicolor were injected in cigarettes,and calculated the transfer rates.The results showed that the transfer rate of EPS in gas in the rage of 1.26%~12.40% and the adding 0.04% EPS could improve the quality,increase the amount of aroma,enhanced sense of sweet,raise sophistication and cover the smoking foreign gas,The results provided a strong support for the application of EPS in cigarette industry.
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References
-
[1]
孟庆虹,张守文.真菌多糖的研究进展[J].粮食与食品工业,2003(3):42.
-
[2]
樊锦艳,王秋颖.食药用真菌多糖的研究及开发利用[J].食品工业科技,2003(12):106.
-
[3]
杜庆.食(药)用真菌多糖的研究进展[J].中国食物与营养,2011,17(5):75.
-
[4]
郭振楚.糖类化学[M].化学工业出版社,2005.
-
[5]
王凯,朱瑞芝,向能军,等.卷烟制品中糖类物质的热解研究进展[J].光谱实验室,2009(5):1289.
-
[6]
周繇.中国长白山植物资源志[M].北京:中国林业出版社,2010.
-
[7]
王永敏,祝文兴,安利国,等.蛹虫草胞外多糖的提取纯化及免疫活性研究[J].食品与药品,2009,11(3):8.
-
[8]
齐慧玲,魏绍云,王继伦,等.Sevag法去除蛋白及多糖中蛋白的研究[J].天津化工,2000(3):20.
-
[9]
Masuko T,Minami A,Iwasaki N,et al.Carbohydrate analysis by a phenol-sulfuricacidmethod in microplate format[J].Analytical Biochemistry,2005,339(1):69.
-
[10]
宋瑜冰,宗永立,谢剑平,等.一些酯类香料在卷烟中的转移研究[J].烟草科技,2005(6):22.
-
[11]
姬小明,刘云,刘乐,等.5-木糖酯类衍生物的合成及其卷烟加香应用[J].科技导报2010,21:68.
-
[12]
王吉中,耿卢静,席攀攀,等.发酵产云芝胞外多糖的分析及其在卷烟中的应用[J].食品工业科技,2012(8):140.
-
[13]
王吉中,杨琛琛, 耿卢静,等.硬毛盖孔菌胞外多糖组分分析及其在卷烟烟气中的转移率[J].烟草化学,2012(6):52.
-
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