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分子生物学技术在堆肥微生物研究中的应用综述

张蔓 ,  魏明宝 ,  马闯 ,  张宏忠 ,  赵继红

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张蔓, 魏明宝, 马闯, 等. 分子生物学技术在堆肥微生物研究中的应用综述[J]. 轻工学报, 2013, 28(3): 13-19. doi: 10.3969/j.issn.2095-476X.2013.03.004
引用本文: 张蔓, 魏明宝, 马闯, 等. 分子生物学技术在堆肥微生物研究中的应用综述[J]. 轻工学报, 2013, 28(3): 13-19. doi: 10.3969/j.issn.2095-476X.2013.03.004
shu
ZHANG Man, WEI Ming-bao, MA Chuang, et al. Review of application of molecular biotechnology in compost microorganism research[J]. Journal of Light Industry, 2013, 28(3): 13-19. doi: 10.3969/j.issn.2095-476X.2013.03.004
Citation: ZHANG Man, WEI Ming-bao, MA Chuang, et al. Review of application of molecular biotechnology in compost microorganism research[J]. Journal of Light Industry, 2013, 28(3): 13-19. doi: 10.3969/j.issn.2095-476X.2013.03.004
shu

分子生物学技术在堆肥微生物研究中的应用综述

  • 郑州轻工业学院 材料与化学工程学院, 河南 郑州 450001;

  • 河南省表界面科学重点实验室, 河南 郑州 450001

  • 基金项目: 河南省重大公益项目(101100910300)
    国家水体污染控制与治理科技重大专项子课题(2012ZX07204-001)

  • 中图分类号: Q71;Q75;Q785

Review of application of molecular biotechnology in compost microorganism research

  • College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;

  • He'nan Provincial Key Lab of Surface and Interface Science, Zhengzhou 450001, China

  • Received Date: 2013-03-26
    Available Online: 2013-05-15

    CLC number: Q71;Q75;Q785

  • 摘要: 对常用的16S/18S rRNA/DNA序列分析、变性梯度凝胶电泳、DNA单链构象多态性分析、限制性片段长度多态性分析、随机扩增多态性DNA分析、扩增的限制性片段长度多态性分析等分子生物学技术的特点及其在堆肥微生物研究中的应用进行了述评,提出堆肥微生物研究应结合传统的分离培养方法,多种技术互补使用,以便取得更科学的结果.
    Abstract: The molecular biotechnology characteristics and application in compost microorganism research were reviewed,including 16S/18S rRNA/DNA sequence analysis,DGGE,SSCP,RFLP,RAPD and AFLP technology.In the future research of compost microorganism,we will complementarily use the traditional separation method and combine molecular biotechnology to achieve a better science result.
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  • 收稿日期:  2013-03-26
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张蔓, 魏明宝, 马闯, 等. 分子生物学技术在堆肥微生物研究中的应用综述[J]. 轻工学报, 2013, 28(3): 13-19. doi: 10.3969/j.issn.2095-476X.2013.03.004
引用本文: 张蔓, 魏明宝, 马闯, 等. 分子生物学技术在堆肥微生物研究中的应用综述[J]. 轻工学报, 2013, 28(3): 13-19. doi: 10.3969/j.issn.2095-476X.2013.03.004
shu
ZHANG Man, WEI Ming-bao, MA Chuang, et al. Review of application of molecular biotechnology in compost microorganism research[J]. Journal of Light Industry, 2013, 28(3): 13-19. doi: 10.3969/j.issn.2095-476X.2013.03.004
Citation: ZHANG Man, WEI Ming-bao, MA Chuang, et al. Review of application of molecular biotechnology in compost microorganism research[J]. Journal of Light Industry, 2013, 28(3): 13-19. doi: 10.3969/j.issn.2095-476X.2013.03.004
shu

分子生物学技术在堆肥微生物研究中的应用综述

  • 郑州轻工业学院 材料与化学工程学院, 河南 郑州 450001;
  • 河南省表界面科学重点实验室, 河南 郑州 450001
  • 收稿日期: 2013-03-26
  • 网络出版日期: 2013-05-15
基金项目:  河南省重大公益项目(101100910300)国家水体污染控制与治理科技重大专项子课题(2012ZX07204-001)

摘要: 对常用的16S/18S rRNA/DNA序列分析、变性梯度凝胶电泳、DNA单链构象多态性分析、限制性片段长度多态性分析、随机扩增多态性DNA分析、扩增的限制性片段长度多态性分析等分子生物学技术的特点及其在堆肥微生物研究中的应用进行了述评,提出堆肥微生物研究应结合传统的分离培养方法,多种技术互补使用,以便取得更科学的结果.

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