节杆菌Z3粗酶液降解白肋烟烟碱条件优化
Conditions optimization of Arthrobacter Z3 crude enzyme solution degradate nicotine in the Burley tobacco
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摘要: 以节杆菌Z3(Arthrobacter Z3)为产酶菌株,通过正交实验,对烟碱脱氢酶降解白肋烟烟碱条件进行了研究.实验结果表明,最佳发酵条件为:酶液添加量50%,发酵温度33℃,发酵时间9 h,烟丝含水率70%.此条件下烟碱降解率为49.32%,使白肋烟烟碱含量达到了烤烟水平.这表明,粗酶液能显著降低白肋烟中的烟碱.Abstract: Using the Arthrobacter Z3 as enzyme production strains,the conditions of nicotine dehydrogenase degradation Burley nicotine was studied by orthogonal experiment.The results showed that the best fermentation conditions were:50% of the added amount of the enzyme solution, fermentation temperature 33℃, fermentation time 9 h, tobacco shreds moisture content 70%.Under these conditions nicotine degradation rate was 49.32% and Burley nicotine content reached the same level of flue-cured tobacco.The crude enzyme fluid can significantly reduce nicotine in Burley tobacco.
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Key words:
- Burley tobacco /
- nicotine degradation /
- fermentation condition /
- Arthrobacter Z3
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