响应面法优化烟叶中蔗糖酯的超声波萃取工艺
Optimization on ultrasonic-assisted extraction technology of sucrose esters from tobacco by response surface methodology
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摘要: 对烟叶中蔗糖酯(SE)的超声波萃取工艺进行了研究.以气相色谱-质谱(GC-MS)法作为分析手段,采用响应面法考察了萃取溶剂、温度、时间和料液比等因素对萃取SE的影响.结果表明,烟叶中SE的最佳萃取条件为:以乙腈为萃取溶剂,料液比为1:29,萃取温度为43℃,时间16 min.在此条件下,烟叶中SE总萃取量的验证值为5.039,理论预测值为5.067;SE的平均回收率为99.87%,与单因素最佳条件下的平均回收率(92.13%)相比提高了7.74%.对10种烟叶样品测定的结果表明,烟叶中SE的种类和含量因产地、品种、等级的不同而有所不同.Abstract: The ultrasonic-assisted extraction technology of sucrose esters (SE) from tobacco was studied.The qualitative and quantitative analysis was performed by gas chromatography-mass spectrometry (GC-MS) method,and the effect of various factors including extraction solvent, temperature,time and solid liquid ratio on the total extract yield of SE was investigated by response surface methodology (RSM).The results showed that the optimal extraction conditions were as follows:the extraction solvent acetonitrile,the solid liquid ratio 1:29,the temperature 43℃ and the extraction time 16 min.Under the optimized conditions,the total extract yield of SE was 5.039 (actual) or 5.067 (theoretical),the average recovery was 99.87%,7.74% more than that obtained under the optimized conditions of single facter tests.The determination results of 10 kinds of tobacco leaf showed that the kinds and content of SE in tobacco leaves were different with different origin,variety and level.
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Key words:
- tobacco /
- sucrose ester /
- ultrasonic extraction /
- GC-MS /
- response surface methodology
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