响应面法优化烟叶中蔗糖酯的超声波萃取工艺

陈凤华; 李山; 贾春晓; 王瑞玲; 王莹莹; 毛多斌

doi:10.3969/j.issn.2095-476X.2013.04.004

CN 41-1437/TS ISSN 2096-1553

响应面法优化烟叶中蔗糖酯的超声波萃取工艺

陈凤华 , 李山 , 贾春晓 , 王瑞玲 , 王莹莹 , 毛多斌

陈凤华, 李山, 贾春晓, 等. 响应面法优化烟叶中蔗糖酯的超声波萃取工艺[J]. 轻工学报, 2013, 28(4): 15-19. doi: 10.3969/j.issn.2095-476X.2013.04.004

引用本文: 陈凤华, 李山, 贾春晓, 等. 响应面法优化烟叶中蔗糖酯的超声波萃取工艺[J]. 轻工学报, 2013, 28(4): 15-19. doi: 10.3969/j.issn.2095-476X.2013.04.004

CHEN Feng-hua, LI Shan, JIA Chun-xiao, et al. Optimization on ultrasonic-assisted extraction technology of sucrose esters from tobacco by response surface methodology[J]. Journal of Light Industry, 2013, 28(4): 15-19. doi: 10.3969/j.issn.2095-476X.2013.04.004

Citation: CHEN Feng-hua, LI Shan, JIA Chun-xiao, et al. Optimization on ultrasonic-assisted extraction technology of sucrose esters from tobacco by response surface methodology[J]. Journal of Light Industry, 2013, 28(4): 15-19. doi: 10.3969/j.issn.2095-476X.2013.04.004

响应面法优化烟叶中蔗糖酯的超声波萃取工艺

郑州轻工业学院材料与化学工程学院, 河南郑州 450001;
郑州轻工业学院烟草科学与工程学院, 河南郑州 450001

通讯作者: 贾春晓

基金项目: 中国烟草总公司卷烟增香保润重大科技专项(110201101025(BR-07))
中图分类号: TS424;TQ651.2

Optimization on ultrasonic-assisted extraction technology of sucrose esters from tobacco by response surface methodology

College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China

Corresponding author: JIA Chun-xiao,

Received Date: 2013-06-01
Available Online: 2013-07-15
CLC number: TS424;TQ651.2

摘要: 对烟叶中蔗糖酯(SE)的超声波萃取工艺进行了研究.以气相色谱-质谱(GC-MS)法作为分析手段,采用响应面法考察了萃取溶剂、温度、时间和料液比等因素对萃取SE的影响.结果表明,烟叶中SE的最佳萃取条件为:以乙腈为萃取溶剂,料液比为1:29,萃取温度为43℃,时间16 min.在此条件下,烟叶中SE总萃取量的验证值为5.039,理论预测值为5.067;SE的平均回收率为99.87%,与单因素最佳条件下的平均回收率(92.13%)相比提高了7.74%.对10种烟叶样品测定的结果表明,烟叶中SE的种类和含量因产地、品种、等级的不同而有所不同.
- 烟叶 /
- 蔗糖酯 /
- 超声波萃取法 /
- 气相色谱-质谱联用 /
- 响应面法
Abstract: The ultrasonic-assisted extraction technology of sucrose esters (SE) from tobacco was studied.The qualitative and quantitative analysis was performed by gas chromatography-mass spectrometry (GC-MS) method,and the effect of various factors including extraction solvent, temperature,time and solid liquid ratio on the total extract yield of SE was investigated by response surface methodology (RSM).The results showed that the optimal extraction conditions were as follows:the extraction solvent acetonitrile,the solid liquid ratio 1:29,the temperature 43℃ and the extraction time 16 min.Under the optimized conditions,the total extract yield of SE was 5.039 (actual) or 5.067 (theoretical),the average recovery was 99.87%,7.74% more than that obtained under the optimized conditions of single facter tests.The determination results of 10 kinds of tobacco leaf showed that the kinds and content of SE in tobacco leaves were different with different origin,variety and level.
- tobacco /
- sucrose ester /
- ultrasonic extraction /
- GC-MS /
- response surface methodology
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1.
沈阳化工大学材料科学与工程学院沈阳 110142

陈凤华, 李山, 贾春晓, 等. 响应面法优化烟叶中蔗糖酯的超声波萃取工艺[J]. 轻工学报, 2013, 28(4): 15-19. doi: 10.3969/j.issn.2095-476X.2013.04.004

引用本文: 陈凤华, 李山, 贾春晓, 等. 响应面法优化烟叶中蔗糖酯的超声波萃取工艺[J]. 轻工学报, 2013, 28(4): 15-19. doi: 10.3969/j.issn.2095-476X.2013.04.004

响应面法优化烟叶中蔗糖酯的超声波萃取工艺

通讯作者: 贾春晓

郑州轻工业学院材料与化学工程学院, 河南郑州 450001;
郑州轻工业学院烟草科学与工程学院, 河南郑州 450001

收稿日期: 2013-06-01
网络出版日期: 2013-07-15

基金项目: 中国烟草总公司卷烟增香保润重大科技专项(110201101025(BR-07))

关键词:

摘要: 对烟叶中蔗糖酯(SE)的超声波萃取工艺进行了研究.以气相色谱-质谱(GC-MS)法作为分析手段,采用响应面法考察了萃取溶剂、温度、时间和料液比等因素对萃取SE的影响.结果表明,烟叶中SE的最佳萃取条件为:以乙腈为萃取溶剂,料液比为1:29,萃取温度为43℃,时间16 min.在此条件下,烟叶中SE总萃取量的验证值为5.039,理论预测值为5.067;SE的平均回收率为99.87%,与单因素最佳条件下的平均回收率(92.13%)相比提高了7.74%.对10种烟叶样品测定的结果表明,烟叶中SE的种类和含量因产地、品种、等级的不同而有所不同.

English Abstract

Optimization on ultrasonic-assisted extraction technology of sucrose esters from tobacco by response surface methodology

Corresponding author: JIA Chun-xiao,

College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China

Received Date: 2013-06-01
Available Online: 2013-07-15

Abstract: The ultrasonic-assisted extraction technology of sucrose esters (SE) from tobacco was studied.The qualitative and quantitative analysis was performed by gas chromatography-mass spectrometry (GC-MS) method,and the effect of various factors including extraction solvent, temperature,time and solid liquid ratio on the total extract yield of SE was investigated by response surface methodology (RSM).The results showed that the optimal extraction conditions were as follows:the extraction solvent acetonitrile,the solid liquid ratio 1:29,the temperature 43℃ and the extraction time 16 min.Under the optimized conditions,the total extract yield of SE was 5.039 (actual) or 5.067 (theoretical),the average recovery was 99.87%,7.74% more than that obtained under the optimized conditions of single facter tests.The determination results of 10 kinds of tobacco leaf showed that the kinds and content of SE in tobacco leaves were different with different origin,variety and level.

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