均匀试验优化黑蒜提取液的抗氧化活性
Antioxidant activity optimization of black garlic extract based on uniform design
-
摘要: 以DPPH自由基清除率为指标,利用均匀试验考察了提取温度、时间、pH和底物质量比对黑蒜提取液抗氧化活性的影响.结果表明,pH对黑蒜提取液抗氧化活性具有显著影响(p<0.05).黑蒜提取液制取的最佳工艺条件为:提取温度40℃,提取时间30 min,pH=3.0,黑蒜与去离子水的质量比(g GA6FA mL)为1 GA6FA 25,此时对DPPH自由基的清除率为92.65%,与理论值的相对误差3.59%.Abstract: With DPPH radical-scavenging rate as the indicator, the effects of temperature, time, pH value and substrates mass ratio on the antioxidant activity of black garlic extract were studied by using the uniform design.The result showed that the pH value had a significant influence (p<0.05) on the antioxidant activity of black garlic extract, and the optimal extracting conditions were temperature 40℃, retention time 30 min, pH=3.0 and mass ratio of black garlic to deionized water 1 GA6FA 25 (g GA6FA mL).Under these conditions, the DPPH radical-scavenging rate was 92.65%, which had a relative error of 3.59% to the theoretical value.
-
Key words:
- black garlic /
- antioxidant activity /
- uniform design
-
-
[1]
Khoo Y,Aziz Z.Garlic supplementation and serum cholesterol:a metaanalysis[J].J Clin Pharm Ther,2009,34:133.
-
[2]
Pal R,Vaiphei K,Sikander A,et al.Effect of garlic on isoniazid and rifampicin-induced hepatic injury in rats[J].World J Gastroenterol,2006,12:636.
-
[3]
Shinkawa H,Takemura S,Minamiyama Y,et al.S-allylcysteine is effective as a chemopreventive agent against porcine serum-induced hepatic fibrosis in rats[J].Osaka City Med,2009,55:61.
-
[4]
何荣海,马海乐.大蒜辣素超声辅助提取的试验研究[J].食品科学,2006,27(2):147.
-
[5]
陆广念,朱志雄,宋晓敏.常见蔬菜抗氧化活性与总酚含量的研究[J].食品科技,2009,34(9):68.
-
[6]
Ackermann R T,Mulrow C D,Ramirez G,et al.Garlic shows promise for improving some cardiovascular risk factors[J].Arch Int Med,2001,161:813.
-
[7]
Chowdhury R,Dutta A,Chaudhuri S R,et al.In vitro and in vivo reduction of sodium arsenite induced toxicity by aqueous garlic extract[J].Food Chem Toxicol,2008,46:740.
-
[8]
周广勇,缪冶炼,陈介余,等.黑大蒜贮藏中主要成分和自由基清除能力的变化[J].中国食品学报,2010,10(6):64.
-
[9]
Das I,Saha T.Effect of garlic on lipid peroxidation and antioxidation enzymes in DMBA-induced skin carcinoma[J].Nutrition,2009,25:459.
-
[10]
陆茂松,闵吉梅,王夔.大蒜有机硫化物的研究(Ⅱ)[J].中草药,2002,33(12):1059.
-
[11]
Rivlin R S.Historical perspectives on the use of garlic[J].J Nutr,2001,131:954.
-
[12]
Sato E,Kohno M,Hamano H,et a1.Increased antioxidative potency of garlic by spontaneous short-term fermentation[J].Plant Foods Hum Nutr,2006,61:157.
-
[13]
Kim S H,Jung E Y,Kang D H,et al.Physical stability,antioxidative properties,and photoprotective effects of a functionalized formulation containing black garlic extract[J].Journal of Photochemistry and Photobiology B:Bio-logy,2012,117:104.
-
[14]
章银良,周文权.均匀试验优化酪蛋白-木糖美拉德提取产物的抗氧化活性[J].食品工业,2013,13(1):27.
-
[15]
潘丽军,章银良.试验设计与数据处理[M].南京:东南大学出版社,2008.
-
[1]
计量
- PDF下载量: 28
- 文章访问数: 925
- 引证文献数: 0