JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 29 Issue 4
July 2014
Article Contents
WANG Ming-hua, YANG Guang, ZHANG Yuan-chang, et al. DNA immobilization and hybridization on graphene/gold nanoparticles/polypyrrole nanocomposite[J]. Journal of Light Industry, 2014, 29(4): 6-11. doi: 10.3969/j.issn.2095-476X.2014.04.002
Citation: WANG Ming-hua, YANG Guang, ZHANG Yuan-chang, et al. DNA immobilization and hybridization on graphene/gold nanoparticles/polypyrrole nanocomposite[J]. Journal of Light Industry, 2014, 29(4): 6-11. doi: 10.3969/j.issn.2095-476X.2014.04.002 shu

DNA immobilization and hybridization on graphene/gold nanoparticles/polypyrrole nanocomposite

  • Corresponding author: ZHANG Zhi-hong, 
  • Received Date: 2014-06-13
    Available Online: 2014-07-15
  • Graphene/gold nanoparticles/polypyrrole (G/AuNPs/PPy) nanocomposite was prepared by in-situ chemical oxidation method,then DNA was immobilized and hybridized on the surface of the composite.The chemical structure,element composition and surface morphology of the nanocomposite were characterized by Fourier transform infrared spectroscopy,X-ray photoelectron spectroscopy,field emission scanning electron microscope and transmission electron microscopy,respectively.Afterwards,electrochemical cyclic voltammetry,impedance spectroscopy and electrochemical quartz crystal microbalance were used to determine the variation of electrochemical properties and weight of the nanocomposite before and after DNA immobilization/hybridization.It demonstrated that the electrochemical activities of G/AuNPs/PPy were better than that of G/AuNPs nanocomposite.The immobilization amount of DNA on the G/AuNPs/PPy nanocomposite was 307 ng,and aslo 357 ng of perfectly matched target DNA was detected on the surface.
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