DENG Mei-zhong, CHEN Du-jian, HUANG Sheng-han and et al. Determination of 3,4,5-trihydroxy benzoic acid in tobacco essence and flavor[J]. Journal of Light Industry, 2014, 29(4): 39-42. doi: 10.3969/j.issn.2095-476X.2014.04.009
Citation:
DENG Mei-zhong, CHEN Du-jian, HUANG Sheng-han and et al. Determination of 3,4,5-trihydroxy benzoic acid in tobacco essence and flavor[J]. Journal of Light Industry, 2014, 29(4): 39-42.
doi:
10.3969/j.issn.2095-476X.2014.04.009
Determination of 3,4,5-trihydroxy benzoic acid in tobacco essence and flavor
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Received Date:
2014-03-21
Available Online:
2014-07-15
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Abstract
A method for the detection of 3,4,5-trihydroxy benzoic acid in tobacco essence and flavor was developed by using high performance liquid chromatography.After oscillate-extraction by ethanol,the samples were separated by a C18 column (100 mm×3.9 mm,5 μm),using methanol and 0.05% phosphoric acid (6:94) as mobile phases by isocratic elution,and detected by diode array detector with the wavelength of 272 nm.The experimental results showed that:within the range of 0.1~10.0 mg/L,the linear correlation coefficient for 3,4,5-trihydroxy benzoic acid was 0.999 4,the low detection limit was 1.01 μg/g,the recovery was 93.1%~103.2%,the RSD < 5% (n=5).The method was sensitive,accurate and easy-controlled,and it was suitable for quantitive determination of 3,4,5-trihydroxy benzoic acid in different types of tobacco essence and flavor.
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References
-
[1]
吕喆, 龚守良,牛凤兰,等.3,4,5-三羟基苯甲酸对肿瘤细胞凋亡及细胞周期进程的影响[J].吉林大学学报:医学版,2008,34(1):90.
-
[2]
赵文静, 牛凤兰,刘作家.3,4,5-三羟基苯甲酸对H22肝癌小鼠实体瘤的抑制作用及其机制[J].吉林大学学报:医学版,2010,36(1):79.
-
[3]
李俊,周彩霞,周小波,等.高效液相色谱法测定丁香酸及其杂质含量[J].分析科学学报,2000,16(2):142.
-
[4]
李刚,杨晓虹, 王艳,等.分离和鉴定草本水杨梅中芳香类化合物[J].吉林大学学报:医学版,2006,32(2):196.
-
[5]
郗艳丽,马颖哲,牛凤兰,等.HPLC测定3,4,5-三羟基苯甲酸脂质体药物含量及包封率[J].中国药学杂志,2010,15(2):101.
-
[6]
韩林涛,黄芳.高效液相色谱法测定克淋通分散片中没食子酸含量的方法学研究[J].湖北中医学院学报,2008,10(2):46.
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Proportional views
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