ZHANG Rui, LI Yuan-shi, KOU Xiao-teng, et al. Separation and purification of laccase used for tobacco stem lignin degradation[J]. Journal of Light Industry, 2014, 29(6): 5-8,12. doi: 10.3969/j.issn.2095-476X.2014.06.002
Citation:
ZHANG Rui, LI Yuan-shi, KOU Xiao-teng, et al. Separation and purification of laccase used for tobacco stem lignin degradation[J]. Journal of Light Industry, 2014, 29(6): 5-8,12.
doi:
10.3969/j.issn.2095-476X.2014.06.002
Separation and purification of laccase used for tobacco stem lignin degradation
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College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
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Production and Technical Center, Jilin Tobacco Industry Co., Ltd., Yanji 133000, China;
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Department of Materials Research, China Tobacco Anhui Industry Co., Ltd., Bengbu 233000, China;
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Technology Center, China Tobacco Yunnan Reconstituted Tobacco Co., Ltd., Kunming 650000, China
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Corresponding author:
MA Lin,
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Received Date:
2014-04-22
Available Online:
2014-11-15
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Abstract
After the laccase crude enzyme which was obtained by fermenting Coriolus versicolor had been concentrated and hyperfiltrated,grading salting precipitation and DEAE-Sepharose-F.F.column chromatography had been taken to separate and purify the laccase fermented liquid with ammonium sulfate as the salting-out agent.SDS-PAGE electrophoresis results showed that pure laccase could be obtained,the molecular weight was about 64.4 kD,enzyme was purified for 22.80 times by the purification process,the enzyme activity recovery rate was 36.32%.These proved that the laccase which was produced by Coriolus versicolor could be well purified.Using the enzyme solution in the experiment stem lignin degradation,the results showed that the intrinsic quality of the processed tobacco stems had been improved to some extent,miscellaneous gases and irritating significantly reduced,wood gas also significantly reduced,aroma and aftertaste slightly improved.
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