LI Wan-peng, GONG Li-hua and FU Shuang. Uncertainty evaluation in the determination result of ochratoxin A in cereal grains by HPLC[J]. Journal of Light Industry, 2015, 30(3-4): 20-25. doi: 10.3969/j.issn.2095-476X.2015.3/4.005
Citation:
LI Wan-peng, GONG Li-hua and FU Shuang. Uncertainty evaluation in the determination result of ochratoxin A in cereal grains by HPLC[J]. Journal of Light Industry, 2015, 30(3-4): 20-25.
doi:
10.3969/j.issn.2095-476X.2015.3/4.005
Uncertainty evaluation in the determination result of ochratoxin A in cereal grains by HPLC
-
Food Laboratory, He'nan GRG Metrology & Test Co., Ltd., Zhengzhou 450001, China;
-
Consultancy Centre of He'nan Inspection & Quarantine Identification of P. R., Zhengzhou 450003, China
-
Received Date:
2015-04-14
Available Online:
2015-09-15
-
Abstract
An evaluation method for uncertainty in measuring ochratoxin A(OTA) in cereal grains with HPLC was established.Standard uncertainty and expanded uncertainty of the components in the process of the determination were calculated,an evaluation and comparison on the sources of uncertainty and the relative contribution were made. The results showed that the sources of the determination result uncertainty could be attributed to uncertainty of calibration, weighting sample, volume, recovery and testing by instrument. Preparing standard solution of the uncertainty of calibration accounted for the largest proportion of the uncertainty, and the uncertainty of volume accounted for the minimum. The expanded uncertainty was 0.476 μg/kg (coverage factor k=2) when the content of OTA was 3.17 μg/kg in cereal grains.Therefore, testing personnel should prepare standard solution strictly in accordance with the specification, at the same time, hold good critical control points of pretreatment process steps such as extraction, purification, elution and so on, in order to control the uncertainty of the determination results in an acceptable range.
-
-
References
-
[1]
陈荔红.高效液相色谱法测定全脂乳粉中苯甲酸含量的测定不确定度评定[J].质量技术监督研究,2011(5):27.
-
[2]
常晨阳,王碧生,方成俊,等.LC-MS/MS法测定动物源食品中呋喃西林代谢物不确定度的评定[J].现代农业科技,2014(12):274.
-
[3]
钟帼歆,富志军.LC-MS法测定猪肉中喹诺酮类药物残留的不确定度评定[J].海峡药学,2014,26(2):63.
-
[4]
倪永付,闫秋成,朱莉萍,等.LC-MS/MS法测定鲜姜中涕灭威及其代谢物的不确定度评定[J].食品研究与开发,2013,34(23):95.
-
[5]
Robertson M,Chan T S S.APLAC interpretation and guidance on the estimation of uncertainty of measurement in testing[J].Journal of AOAC International,2003,86(5):1070.
-
[6]
Kuang Y C,Rajan A,Ooi M P L,et al.Standard uncertainty evaluation of multivariate polynomial[J].Measurement,2014,58:483.
-
Proportional views
-
-
DownLoad: