食源性致病菌分子生物学检测技术研究进展
Progress in molecular biology detection technology for food-borne pathogens
-
摘要: 随着分子生物学的不断进步,对食源性致病菌的检测已经发展到了研究生物大分子的阶段.综观目前出现的一些检测食源性致病菌的新型技术,发现DNA探针技术、PCR技术等检测技术,具有敏感、特异和快速的特点,已经成为食源性致病菌检测的重要工具.未来食源性致病菌的检测将向着高敏感性、高特异性、操作便捷的方向发展,这就依赖于目前已有技术的改进创新、多种检测技术的联合使用及一些新技术和新方法的出现.Abstract: Along with the advance of molecular biology, the detection of food-borne pathogens have been developed to study phase of biological macromolecules. Through summarizing some new detection technologies for food-borne pathogens it was found that DNA probe techniques, PCR techniques et al, had became important tools for detection of food-borne pathogens with their characteristics of sensitivity, specificity and rapidness. The future detection of food-borne pathogens would toward the direction of high sensitivity, high specificity, and convenient operation, which depended on the improvement and innovation of the existing technology, combined use of a variety of detection technology and the emergence of some new technology and new methods.
-
Key words:
- food-borne pathogens /
- molecular biology /
- detection technology
-
-
[1]
Ikeda M,Yamaguchi N,Tani K,et al.Rapid and simple detection of food poisoning bacteria by bead assay with a microfluidic chip-based system[J].Journal of Microbiological Methods,2006,67(2):241.
-
[2]
García-Cano I,Serrano-Maldonado C E,Olvera-García M,et al.Antibacterial activity produced by Enterococcus spp.isolated from an artisanal Mexican dairy product,Cotija cheese[J].LWT-Food Science and Technology,2014,59(1):26.
-
[3]
Zunabovic M,Domig K J,Kneifel W.Practical relevance of methodologies for detecting and tracing of Listeria monocytogenes in ready-to-eat foods and manufacture environments-A review[J].LWT-Food Science and Technology,2011,44(2):351.
-
[4]
任慧婧,刘芸,王美会,等.志贺氏菌检测方法的研究进展[J].河南农业,2015(14):52.
-
[5]
孙强,李昱洁,任科研,等.大肠杆菌O157:H7检测技术的概况[J].吉林畜牧兽医,2013(7):61.
-
[6]
田晓艳,刘长江,刘延吉,等.生物技术在食品质量与安全中的应用[J].食品科学,2010,31(Z1):160.
-
[7]
谢修志.生物技术在食品检测方面的应用[J].生物技术通报,2010,(1):68.
-
[8]
Zeng Y,Wan Y,Zhang D,et al.A novel magneto-DNA duplex probe for bacterial DNA detection based on exonuclease III-aided cycling amplification[J].Talanta,2015,132:59.
-
[9]
Wei J,Zhou XM,Xing D,et al.Rapid and sensitive detection of Vibrio parahaemolyticus in sea foods by electrochemiluminescence polymerase chain reaction method[J].Food Chemistry,2010,123(3):852.
-
[10]
Wei J,Zhou X,Xing D,et al.Rapid and sensitive detection of Vibrio parahaemolyticus in sea foods by electrochemiluminescence polymerase chain reaction method[J].Food Chemistry,2010,123(3):852.
-
[11]
巢强国,杨学明,葛宇,等.PCR法检测食品中大肠杆菌O157:H7[J].食品科学,2010,31(8):212.
-
[12]
Bonnstetter K K,Wolter D J,Tenover F C,et al.Rapid multiplex PCR assay for identification of USA300 community-associated methicillin-resistant Staphylococcus aureus isolates[J].Journal of Clinical Microbiology,2007,45(1):141.
-
[13]
王攀,王萍,高林.PCR法快速检测三种食源性致病菌的研究[J].食品安全质量检测学报,2013,4(3):917.
-
[14]
Sjöling Å,Sadeghipoorjahromi L,Novak D,et al.Detection of major diarrheagenic bacterial pathogens by multiplex PCR panels[J].Microbiological Research,2015,172:34.
-
[15]
万志刚,汤慕瑾,吕敬章,等.多种食源性致病菌检测的多重PCR方法的研究[J].现代生物医学进展,2012,12(11):2177.
-
[16]
蔡军,李慧,欧静堃,等.3种食源性致病菌多重PCR检测体系的建立[J].食品科技, 2015(3):324.
-
[17]
楼秀芹,斯国静,戚建江,等.食品中阪崎肠杆菌多重PCR检测方法的建立[J].中国卫生检验杂志,2014,24(2):159.
-
[18]
Cano-Gomez A,Hoj L,Owens L,et al.A multiplex PCR-based protocol for identification and quantification of Vibrio harveyi-related species[J].Aquaculture,2015,437:195.
-
[19]
张惟材.生物实验室系列实时荧光定量PCR[M].北京:化学工业出版社,2013:5-10.
-
[20]
武鑫,张宇霞,史晗,等.致病性沙门菌SYBR GreenⅠ实时荧光定量PCR检测方法的建立[J].中国兽医科学,2015,45(3):270.
-
[21]
胡朝友,傅春玲,陆巧荣,等.TaqMan探针荧光PCR定量检测大肠埃希菌方法研究[J].现代预防医学,2014,41(6):1070.
-
[22]
韩春来.三种食源性致病菌多重荧光PCR检测方法的建立[J].家禽科学,2012(8):11.
-
[23]
张驰,杨军,刘新梅,等.食品中3种致病菌的Taqman多重荧光定量PCR检测[J].食品研究与开发,2011,32(4):151.
-
[24]
刘静宇,凌莉,邓翼惠,等.活的非可培养状态副溶血性弧菌实时荧光逆转录PCR检测方法的建立及其毒力研究[J].中国食品卫生杂志,2013,25(4):309.
-
[25]
Molaee N,Abtahi H,Ghannadzadeh M J,et al.Application of reverse transcriptase-PCR(RT-PCR) for rapid detection of viable Escherichia coli in drinking water samples[J].Journal of Environmental Health Science and Engineering,2015,13(1):24.
-
[26]
Amaro Filho S M,Nicol A F.The utility of in situ detection,including RT in situ PCR of viral nucleic acid and the co-localization of the cytokine response to the study of viral pathogenesis[J].Methods,2010,52(4):332.
-
[27]
吴彩云,蔡俊鹏,杨汝德.结合流式细胞仪检测技术的菌体原位PCR扩增[J].微生物学报,2004,44(3):399.
-
[28]
彭乃才.环介导等温扩增技术检测食源性致病菌的研究进展[J].肉类工业,2014(5):32.
-
[29]
张亚爽.LAMP和PCR检测单核细胞增生性李斯特氏菌的研究[D].保定:河北农业大学,2008.
-
[30]
徐义刚,李苏龙,李丹丹,等.食品中金黄色葡萄球菌DNA环介导恒温扩增快速检测方法的建立与应用[J].中国农业科学,2010,43(8):1655.
-
[31]
陈传,凌霄,郭震,等.LAMP技术在食源性病原微生物检测中的应用[J].生命科学研究,2014,18(2):140.
-
[32]
孟勋.生物芯片及其应用研究[J].中国科技信息,2015(3):35.
-
[33]
Stears R L,Martinsky T,Schena M.Trends in microarray analysis[J].Nature Medicine,2003,9(1):140.
-
[34]
Zhang H,Zhang Y,Lin Y,et al.Ultrasensitive detection and rapid identification of multiple foodborne pathogens with the naked eyes[J].Biosensors and Bioelectronics,2015,71:186.
-
[35]
高兴,辛文文,高姗,等.11种(株)食源性细菌基因芯片检测方法的建立[J].生物技术通报,2013(12):123.
-
[36]
孔金明.生物芯片技术在食品安全领域的应用综述[J].郑州轻工业学院学报:自然科学版,2013,28(1):1.
-
[37]
王颖.生物芯片技术及其应用研究[J].科学教育,2010,16(1):91.
-
[38]
王亚丽,蔡阳,刘韬,等.金黄色葡萄球菌液相芯片检测方法的建立及应用[J].中国生物制品学杂志,2012,25(10):1383.
-
[39]
尹俐.生物芯片技术在食源性疾病诊断中的应用[J].疾病监测与控制杂志,2010,4(8):457.
-
[1]
-
点击查看大图
计量
- PDF下载量: 43
- 文章访问数: 1419
- 引证文献数: 0
下载:
