食源性致病菌PCR检测技术研究进展

胡金强; 雷俊婷; 景建洲; 孙新城; 高辉; 耿尧; 章银良; 董彩文; 姜春鹏

doi:10.3969/j.issn.2096-1553.2016.3.007

食源性致病菌PCR检测技术研究进展

郑州轻工业学院食品与生物工程学院, 河南郑州 450001;
食品生产与安全河南省协同创新中心, 河南郑州 450001;
河南省食品安全国际联合实验室, 河南郑州 450001
基金项目: 河南省高等学校青年骨干教师资助计划项目(教高[2015]1032号)
郑州轻工业学院青年骨干教师资助计划项目(2013QNGG02)
郑州轻工业学院研究生创新基金项目(2014031)
国家自然科学基金项目(31201901)
郑州轻工业学院博士基金项目(2011BSJJ033)
河南省教育厅科学技术重点研究项目(14A180025)
郑州市科技攻关项目(20130857)
教育部留学回国人员科研启动基金项目(教外司留[2013]693号)
中图分类号: TS207.4

Advance in PCR detection technologies for foodborne pathogenic bacteria

School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
He'nan Collaborative Innovation Center for Food Production and Safety, Zhengzhou 450001, China;
He'nan International Joint Laboratory for Food Safety, Zhengzhou 450001, China
Received Date: 2015-05-20
Available Online: 2016-05-15
CLC number: TS207.4

摘要: 食源性致病菌是诱发食品安全问题的重要隐患,在众多食源性致病菌检测技术中,PCR检测技术因具有特异、敏感、简便、快速等优点而得以广泛应用.然而现有的包括多重PCR技术、实时荧光定量PCR技术、多重实时荧光定量PCR技术、IMS-多重实时荧光定量PCR技术、PCR-ELISA技术、EMA/PMA-PCR技术和DPO-PCR技术等在内的食源性致病菌PCR检测技术及其衍生技术仍存在成本高、效率低、质控差等缺陷,未来应向高灵敏度、高特异性、高通量、高重复性、简易、经济方向发展,以适应食源性致病菌对检测技术的要求.
- 食源性致病菌 /
- PCR检测技术 /
- 食品安全
Abstract: Foodborne pathogenic bacteria is the hidden danger of food safety.During many detection methods,PCR and PCR-derived technologies have advantages of sensible,specific,convenient and rapid,which have been widely used in the detection of foodborne pathogenic bacteria.At present,PCR detection technologies including multiple PCR,real-time PCR,multiple real-time PCR,IMS-multiplex real-time PCR,PCR-ELISA,EMA/PMA-PCR and DPO-PCR have defects such as high cost,low efficiency and poor quality control and need to be impoved to meet the requirements of high sensibility,high specificity,high throughput,high reproducibility,convenience and economics for the detection of foodborne pathogenic bacteria.
- foodborne pathogenic bacteria /
- PCR detection technology /
- food safety
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食源性致病菌PCR检测技术研究进展

Advance in PCR detection technologies for foodborne pathogenic bacteria

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通讯作者: 陈斌, bchen63@163.com

食源性致病菌PCR检测技术研究进展

English Abstract

Advance in PCR detection technologies for foodborne pathogenic bacteria

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食源性致病菌PCR检测技术研究进展

Advance in PCR detection technologies for foodborne pathogenic bacteria

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通讯作者: 陈斌, bchen63@163.com

食源性致病菌PCR检测技术研究进展

English Abstract

Advance in PCR detection technologies for foodborne pathogenic bacteria

目录