WANG Guo-qing, LIU Yun, KE Wei, et al. Screening analysis of antibiotics drugs in animal feed using UPLC-Orbitrap HRMS[J]. Journal of Light Industry, 2016, 31(4): 15-22. doi: 10.3969/j.issn.2096-1553.2016.4.002
Citation:
WANG Guo-qing, LIU Yun, KE Wei, et al. Screening analysis of antibiotics drugs in animal feed using UPLC-Orbitrap HRMS[J]. Journal of Light Industry, 2016, 31(4): 15-22.
doi:
10.3969/j.issn.2096-1553.2016.4.002
Screening analysis of antibiotics drugs in animal feed using UPLC-Orbitrap HRMS
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College of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
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China National Tobacco Quality Supervision and Test Center, Zhengzhou 450000, China
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Received Date:
2016-04-13
Available Online:
2016-07-15
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Abstract
73 kinds of mixed antibacterial drug standards were separated and ionized by ultra high performance liquid chromatography-Orbtrap high resolution mass spectrometry (UPLC-Orbitrap HRMS), the retention times and ionization mode of the the antimicrobial drugs were determined, and the antimicrobial drug accurate measurement of molecular weight and the retention time of the standard database were established.Feed samples were homogenized, first extracted with acetonitrile water solution 50% (v/v), second extracted with acetonitrile, purified by amino solid phase extraction cartridge, dried with nitrogen concentration and filtered for detection.The results showed that the recovery rate of this method was 34.51%~129.64%, the relative standard deviation was 0.1%~24.4%,and add amount of 5 μg·g-1 could be detected.
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