高效液相色谱法同时测定果蔬汁饮料中20种禁限用食品添加剂

刘胜男; 张国庆; 王素方; 郭保宝; 权明珠; 石志格

doi:10.3969/j.issn.2096-1553.2016.4.003

CN 41-1437/TS ISSN 2096-1553

高效液相色谱法同时测定果蔬汁饮料中20种禁限用食品添加剂

刘胜男 , 张国庆 , 王素方 , 郭保宝 , 权明珠 , 石志格

刘胜男, 张国庆, 王素方, 等. 高效液相色谱法同时测定果蔬汁饮料中20种禁限用食品添加剂[J]. 轻工学报, 2016, 31(4): 23-29. doi: 10.3969/j.issn.2096-1553.2016.4.003

引用本文: 刘胜男, 张国庆, 王素方, 等. 高效液相色谱法同时测定果蔬汁饮料中20种禁限用食品添加剂[J]. 轻工学报, 2016, 31(4): 23-29. doi: 10.3969/j.issn.2096-1553.2016.4.003

LIU Sheng-nan, ZHANG Guo-qing, WANG Su-fang, et al. Simultaneous determination of 20 restricted food additives in fruit and vegetable juice beverage by HPLC[J]. Journal of Light Industry, 2016, 31(4): 23-29. doi: 10.3969/j.issn.2096-1553.2016.4.003

Citation: LIU Sheng-nan, ZHANG Guo-qing, WANG Su-fang, et al. Simultaneous determination of 20 restricted food additives in fruit and vegetable juice beverage by HPLC[J]. Journal of Light Industry, 2016, 31(4): 23-29. doi: 10.3969/j.issn.2096-1553.2016.4.003

高效液相色谱法同时测定果蔬汁饮料中20种禁限用食品添加剂

中华人民共和国三门峡出入境检验检疫局, 河南三门峡 472000;
中华人民共和国河南出入境检验检疫局检验检疫技术中心, 河南郑州 450003

通讯作者: 王素方

基金项目: 国家质量监督检验检疫总局科技计划项目（2015IK114）；河南省科技攻关计划项目（152102110025）
中图分类号: TS255.7;O657.63

Simultaneous determination of 20 restricted food additives in fruit and vegetable juice beverage by HPLC

Sanmenxia Entry-exit Inspection and Quarantine Bureau of P. R. China, Sanmenxia 472000, China;
Inspection & Quarantine Technology Centre, He'nan Entry-exit Inspection and Quarantine Bureau of P. R. China, Zhengzhou 450003, China

Corresponding author: WANG Su-fang,

Received Date: 2016-04-13
Available Online: 2016-07-15
CLC number: TS255.7;O657.63

摘要: 研究了同时测定果蔬汁饮料中20种禁限用食品添加剂的高效液相色谱方法：采用甲醇-水提取样品中的添加剂，在ZORBAX SB-C₁₈色谱柱上分离，以甲醇-乙酸铵为流动相进行梯度洗脱，采用二极管阵列检测器多波长分析，外标法定量.结果表明，20种添加剂在0.5～50.0 μg/mL质量浓度范围内线性关系良好，相关系数大于0.9990，方法检出限范围0.5～12.5 mg/kg，定量限范围2.5～40.0 mg/kg，平均加标回收率范围80.1%～100.9%，相对标准偏差1.5%～4.9%.对10种市售果蔬汁饮料中添加剂含量测检结果表明，该方法操作简单、检测添加剂种类多、分离效果好、回收率和精密度均满足要求，适用于果蔬汁饮料中多种添加剂的同时检测.
- 高效液相色谱 /
- 食品添加剂 /
- 果蔬汁饮料
Abstract: A high performance liquid chromatography (HPLC) method was developed for simultaneous determination of 20 restricted food additives in fruit and vegetable juice beverage.The method was based on extraction with methanol mixed with water.The separation was achieved on a ZORBAX SB-C18 column by using methanol and NH4Ac as mobile phase with the gradient eluent.Components were detected with a diode array detector (DAD).The quantification was performed by the external standard method.The results showed that the method exhibited an excellent linearity in the concentration range of 0.5~50.0 μg/mL with a correlation coefficient greater than 0.999 0 and a good repeatability with relative standard deviation of 1.5%~4.9%.The limits of detection were between 0.5 mg/kg and 12.5 mg/kg,the limit of quantitation were between 2.5 mg/kg and 40.0 mg/kg,and average recoveries ranged from 80.1% to 100.9%.The test result of the additives content of ten types of fruit and vegetable juice beverage on the market showed that the present method was simple,and had good separation effect.Its recovery and accuracy could meet the requirements for the simultaneous determination additives in fruit and vegetable juice beverage.
- high performance liquid chromatography (HPLC) /
- food additives /
- fruit and vegetable juice beverage
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沈阳化工大学材料科学与工程学院沈阳 110142

刘胜男, 张国庆, 王素方, 等. 高效液相色谱法同时测定果蔬汁饮料中20种禁限用食品添加剂[J]. 轻工学报, 2016, 31(4): 23-29. doi: 10.3969/j.issn.2096-1553.2016.4.003

高效液相色谱法同时测定果蔬汁饮料中20种禁限用食品添加剂

通讯作者: 王素方

中华人民共和国三门峡出入境检验检疫局, 河南三门峡 472000;
中华人民共和国河南出入境检验检疫局检验检疫技术中心, 河南郑州 450003

收稿日期: 2016-04-13
网络出版日期: 2016-07-15

基金项目: 国家质量监督检验检疫总局科技计划项目（2015IK114）；河南省科技攻关计划项目（152102110025）

关键词:

摘要: 研究了同时测定果蔬汁饮料中20种禁限用食品添加剂的高效液相色谱方法：采用甲醇-水提取样品中的添加剂，在ZORBAX SB-C₁₈色谱柱上分离，以甲醇-乙酸铵为流动相进行梯度洗脱，采用二极管阵列检测器多波长分析，外标法定量.结果表明，20种添加剂在0.5～50.0 μg/mL质量浓度范围内线性关系良好，相关系数大于0.9990，方法检出限范围0.5～12.5 mg/kg，定量限范围2.5～40.0 mg/kg，平均加标回收率范围80.1%～100.9%，相对标准偏差1.5%～4.9%.对10种市售果蔬汁饮料中添加剂含量测检结果表明，该方法操作简单、检测添加剂种类多、分离效果好、回收率和精密度均满足要求，适用于果蔬汁饮料中多种添加剂的同时检测.

English Abstract

Simultaneous determination of 20 restricted food additives in fruit and vegetable juice beverage by HPLC

Corresponding author: WANG Su-fang,

Sanmenxia Entry-exit Inspection and Quarantine Bureau of P. R. China, Sanmenxia 472000, China;
Inspection & Quarantine Technology Centre, He'nan Entry-exit Inspection and Quarantine Bureau of P. R. China, Zhengzhou 450003, China

Received Date: 2016-04-13
Available Online: 2016-07-15

Abstract: A high performance liquid chromatography (HPLC) method was developed for simultaneous determination of 20 restricted food additives in fruit and vegetable juice beverage.The method was based on extraction with methanol mixed with water.The separation was achieved on a ZORBAX SB-C18 column by using methanol and NH4Ac as mobile phase with the gradient eluent.Components were detected with a diode array detector (DAD).The quantification was performed by the external standard method.The results showed that the method exhibited an excellent linearity in the concentration range of 0.5~50.0 μg/mL with a correlation coefficient greater than 0.999 0 and a good repeatability with relative standard deviation of 1.5%~4.9%.The limits of detection were between 0.5 mg/kg and 12.5 mg/kg,the limit of quantitation were between 2.5 mg/kg and 40.0 mg/kg,and average recoveries ranged from 80.1% to 100.9%.The test result of the additives content of ten types of fruit and vegetable juice beverage on the market showed that the present method was simple,and had good separation effect.Its recovery and accuracy could meet the requirements for the simultaneous determination additives in fruit and vegetable juice beverage.

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