高效液相色谱法同时测定果蔬汁饮料中20种禁限用食品添加剂

刘胜男; 张国庆; 王素方; 郭保宝; 权明珠; 石志格

doi:10.3969/j.issn.2096-1553.2016.4.003

July 2016

Article Contents

LIU Sheng-nan, ZHANG Guo-qing, WANG Su-fang, et al. Simultaneous determination of 20 restricted food additives in fruit and vegetable juice beverage by HPLC[J]. Journal of Light Industry, 2016, 31(4): 23-29. doi: 10.3969/j.issn.2096-1553.2016.4.003

Citation: LIU Sheng-nan, ZHANG Guo-qing, WANG Su-fang, et al. Simultaneous determination of 20 restricted food additives in fruit and vegetable juice beverage by HPLC[J]. Journal of Light Industry, 2016, 31(4): 23-29. doi: 10.3969/j.issn.2096-1553.2016.4.003 shu

Simultaneous determination of 20 restricted food additives in fruit and vegetable juice beverage by HPLC

Sanmenxia Entry-exit Inspection and Quarantine Bureau of P. R. China, Sanmenxia 472000, China;
Inspection & Quarantine Technology Centre, He'nan Entry-exit Inspection and Quarantine Bureau of P. R. China, Zhengzhou 450003, China

Corresponding author: WANG Su-fang,
Received Date: 2016-04-13
Available Online: 2016-07-15

Abstract

A high performance liquid chromatography (HPLC) method was developed for simultaneous determination of 20 restricted food additives in fruit and vegetable juice beverage.The method was based on extraction with methanol mixed with water.The separation was achieved on a ZORBAX SB-C18 column by using methanol and NH4Ac as mobile phase with the gradient eluent.Components were detected with a diode array detector (DAD).The quantification was performed by the external standard method.The results showed that the method exhibited an excellent linearity in the concentration range of 0.5~50.0 μg/mL with a correlation coefficient greater than 0.999 0 and a good repeatability with relative standard deviation of 1.5%~4.9%.The limits of detection were between 0.5 mg/kg and 12.5 mg/kg,the limit of quantitation were between 2.5 mg/kg and 40.0 mg/kg,and average recoveries ranged from 80.1% to 100.9%.The test result of the additives content of ten types of fruit and vegetable juice beverage on the market showed that the present method was simple,and had good separation effect.Its recovery and accuracy could meet the requirements for the simultaneous determination additives in fruit and vegetable juice beverage.
- high performance liquid chromatography (HPLC),
- food additives,
- fruit and vegetable juice beverage
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Simultaneous determination of 20 restricted food additives in fruit and vegetable juice beverage by HPLC

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