枸杞多肽的制备及其抗氧化活性研究

李松林; 林静; 蒋长兴; 陈晓明; 王小花; 叶华; 王辉

doi:10.3969/j.issn.2096-1553.2016.6.005

CN 41-1437/TS ISSN 2096-1553

枸杞多肽的制备及其抗氧化活性研究

李松林 , 林静 , 蒋长兴 , 陈晓明 , 王小花 , 叶华 , 王辉

李松林, 林静, 蒋长兴, 等. 枸杞多肽的制备及其抗氧化活性研究[J]. 轻工学报, 2016, 31(6): 31-37. doi: 10.3969/j.issn.2096-1553.2016.6.005

引用本文: 李松林, 林静, 蒋长兴, 等. 枸杞多肽的制备及其抗氧化活性研究[J]. 轻工学报, 2016, 31(6): 31-37. doi: 10.3969/j.issn.2096-1553.2016.6.005

LI Song-lin, LIN Jing, JIANG Chang-xing, et al. Study on preparation and antioxidant activity of the Lycium barbarum polypeptide[J]. Journal of Light Industry, 2016, 31(6): 31-37. doi: 10.3969/j.issn.2096-1553.2016.6.005

Citation: LI Song-lin, LIN Jing, JIANG Chang-xing, et al. Study on preparation and antioxidant activity of the Lycium barbarum polypeptide[J]. Journal of Light Industry, 2016, 31(6): 31-37. doi: 10.3969/j.issn.2096-1553.2016.6.005

枸杞多肽的制备及其抗氧化活性研究

淮阴工学院生命科学与食品工程学院, 江苏淮安 223003;
淮安市科学技术情报研究所, 江苏淮安 223002
基金项目: 江苏省科技厅科技型企业技术创新项目（BC2013427）；淮安市科技支撑计划项目（HAN2015003）
中图分类号: TS255.1

Study on preparation and antioxidant activity of the Lycium barbarum polypeptide

Faculty of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, China;
Institute of Scientific and Technical Information of Huai'an, Huai'an 223003, China
Received Date: 2016-05-28
Available Online: 2016-12-15
CLC number: TS255.1

摘要: 研究了中性蛋白酶和木瓜蛋白酶复合水解制备枸杞活性肽的工艺条件，并采用离子交换层析和凝胶柱层析对酶解产物进行纯化，以制备具有较高抗氧化活力的枸杞多肽.以DPPH·清除率为指标，通过响应面优化得到最佳酶解工艺条件：酶解温度51℃，中性蛋白酶与木瓜蛋白酶质量比1：2.65，酶解时间4.3 h，pH=7.0.此条件下得到的枸杞多肽对DPPH·清除率为（23.32±0.47）%.分别采用DEAE阴离子交换层析和Sephadex G-25凝胶柱层析对枸杞多肽进行分离，最终获得DPPH·和OH·清除率分别为（72.65±1.84）%和（58.72±1.88）%的A-1组分，该组分的DPPH·清除能力、ABTS+·清除能力和OH·清除能力均随浓度的增大而提高.
- 枸杞多肽 /
- 酶解 /
- 纯化 /
- 抗氧化活性
Abstract: Enzymatic preparation conditions of Lycium barbarum bioactive peptides which were derived from hydrolyzation by compound of neutral protease and papain were studied. And then enzymatic hydrolysate was purified by ion-exchange column chromatography and gel filtration chromatography in order to prepare antioxidative bioactive peptides. Based on the DPPH· radical scavenging rate, the preparation method was optimized by response surface methodology. The optimal temperature, composite enzyme quality ratio, time and pH were 51℃,1:2.65, 4.3 h and 7.0, which resulted in a DPPH· radical scavenging rate of (23.32±0.47)%. Then the enzymatic hydrolysate flowed through anion-exchange chromatography and sephdex G-25 gel chromatography in order to get purified polypeptide. Component A-1 was acquired which had the DPPH· and OH· removal rate of (72.65±1.84)% and (58.72±1.88)% respectively. DPPH·, ABTS+· and OH· radical scavenging activity of protein peptide A-1 increased with the increase of concentration.
- Lycium barbarum polypeptide /
- enzymo-lysis /
- purification /
- antioxidant activity
1. [1]
  崔树德.中药大全[M].哈尔滨:黑龙江科学技术出版社,1997.
2. [2]
  于宏.枸杞子的化学成分与生物活性[J]. 国外医药,2007,22(2):45.
3. [3]
  LIU W,LIU Y M,ZHU R,et al.Structure characterization, chemical and enzymatic degradation,and chain conformation of an acidic polysaccharide from Lycium barbarum L.[J].Carbohydrate Polymers,2016,147(20):114.
4. [4]
  MARTINO F,LUCIANA T,CARMELA D A,et al.NMR-based identification of the phenolic profile of fruits of Lycium barbarum (goji berries):Isolation and structural determination of a novel N-feruloyl tyramine dimer as the most abundant antioxidant polyphenol of goji berries[J].Food Chemistry,2016,194:1254.
5. [5]
  张英,唐玉峰,白杰.宁夏枸杞营养成分分析及其应用研究进展[J].保鲜与加工, 2009,9(4):2.
6. [6]
  WANG Z X,LIU Y,LI H,et al.Rice proteins,extracted by alkali and α-amylase,differently affect in vitro antioxidant activity[J].Food Chemistry,2016,206:137.
7. [7]
  KUAMR D,CHATLI M K,SINGH R,et al.Antioxidant and antimicrobial activity of camel milk casein hydrolysates and its fractions[J].Small Ruminant Research,2016,139:20.
8. [8]
  SCHULTZ T P,NICHOLAS D D,HENRY W P,et al.Review of laboratory and outdoor exposure efficacy results of organic biocide:antioxidant combinations, an initial economic analysis and discussion of a proposed mechanism[J].Wood and Fiber Science,2005,37(1):175.
9. [9]
  SCHAICH K M,TIAN X,XIE J.Hurdles and pitfalls in measuring antioxidant efficacy:A critical evaluation of ABTS,DPPH,and ORAC assays[J].Journal of Functional Foods,2015,14:111.
10. [10]
  PARSA A,SALOUT S A.Investigation of the antioxidant activity of electrosynthesized polyaniline/reduced graphene oxide nanocomposite in a binary electrolyte system on ABTS and DPPH free radicals[J].Journal of Electroanalytical Chemistry,2016,760:113.
1. [1]
  章存勇 , 庄海锋 , 时雅琪 , 邹鹏 , 丁乃红 , 纵坤 , 贾良元 , 郭东锋 . 国内外雪茄烟叶热解产物差异性研究. 轻工学报, 2024, 0(0): -.
2. [2]
  章存勇 , 庄海锋 , 时雅琪 , 邹鹏 , 丁乃红 , 纵坤 , 贾良元 , 郭东锋 . 国内外雪茄烟叶热解产物差异性研究. 轻工学报, 2024, 39(5): 118-126. doi: 10.12187/2024.05.014
3. [3]
  张嫚 , 张国治 , 张康逸 , 何梦影 . 超声辅助酶解法制备小麦ACE抑制肽及其稳定性研究. 轻工学报, 2024, 0(0): -.
4. [4]
  张嫚 , 张国治 , 张康逸 , 何梦影 . 超声辅助酶解法制备小麦ACE抑制肽及其稳定性研究. 轻工学报, 2024, 39(5): 29-39. doi: 10.12187/2024.05.004
5. [5]
  池哲翔 , 廖敏 , 史尚 , 李声毅 , 廖芸 , 丁冬 . 国外烟草活性成分提取及纤维材料利用现状与展望. 轻工学报, 2024, 0(0): -.

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沈阳化工大学材料科学与工程学院沈阳 110142

李松林, 林静, 蒋长兴, 等. 枸杞多肽的制备及其抗氧化活性研究[J]. 轻工学报, 2016, 31(6): 31-37. doi: 10.3969/j.issn.2096-1553.2016.6.005

引用本文: 李松林, 林静, 蒋长兴, 等. 枸杞多肽的制备及其抗氧化活性研究[J]. 轻工学报, 2016, 31(6): 31-37. doi: 10.3969/j.issn.2096-1553.2016.6.005

枸杞多肽的制备及其抗氧化活性研究

淮阴工学院生命科学与食品工程学院, 江苏淮安 223003;
淮安市科学技术情报研究所, 江苏淮安 223002

收稿日期: 2016-05-28
网络出版日期: 2016-12-15

基金项目: 江苏省科技厅科技型企业技术创新项目（BC2013427）；淮安市科技支撑计划项目（HAN2015003）

关键词:

摘要: 研究了中性蛋白酶和木瓜蛋白酶复合水解制备枸杞活性肽的工艺条件，并采用离子交换层析和凝胶柱层析对酶解产物进行纯化，以制备具有较高抗氧化活力的枸杞多肽.以DPPH·清除率为指标，通过响应面优化得到最佳酶解工艺条件：酶解温度51℃，中性蛋白酶与木瓜蛋白酶质量比1：2.65，酶解时间4.3 h，pH=7.0.此条件下得到的枸杞多肽对DPPH·清除率为（23.32±0.47）%.分别采用DEAE阴离子交换层析和Sephadex G-25凝胶柱层析对枸杞多肽进行分离，最终获得DPPH·和OH·清除率分别为（72.65±1.84）%和（58.72±1.88）%的A-1组分，该组分的DPPH·清除能力、ABTS+·清除能力和OH·清除能力均随浓度的增大而提高.

English Abstract

Study on preparation and antioxidant activity of the Lycium barbarum polypeptide

Faculty of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, China;
Institute of Scientific and Technical Information of Huai'an, Huai'an 223003, China

Received Date: 2016-05-28
Available Online: 2016-12-15

Abstract: Enzymatic preparation conditions of Lycium barbarum bioactive peptides which were derived from hydrolyzation by compound of neutral protease and papain were studied. And then enzymatic hydrolysate was purified by ion-exchange column chromatography and gel filtration chromatography in order to prepare antioxidative bioactive peptides. Based on the DPPH· radical scavenging rate, the preparation method was optimized by response surface methodology. The optimal temperature, composite enzyme quality ratio, time and pH were 51℃,1:2.65, 4.3 h and 7.0, which resulted in a DPPH· radical scavenging rate of (23.32±0.47)%. Then the enzymatic hydrolysate flowed through anion-exchange chromatography and sephdex G-25 gel chromatography in order to get purified polypeptide. Component A-1 was acquired which had the DPPH· and OH· removal rate of (72.65±1.84)% and (58.72±1.88)% respectively. DPPH·, ABTS+· and OH· radical scavenging activity of protein peptide A-1 increased with the increase of concentration.