JIA Meng-zhu, SUN Jiu-zhe, SU Dong-ying, et al. Tag identification of flue-cured tobacco varieties by multiplex PCR technique[J]. Journal of Light Industry, 2017, 32(3): 51-57. doi: 10.3969/j.issn.2096-1553.2017.3.009
Citation:
JIA Meng-zhu, SUN Jiu-zhe, SU Dong-ying, et al. Tag identification of flue-cured tobacco varieties by multiplex PCR technique[J]. Journal of Light Industry, 2017, 32(3): 51-57.
doi:
10.3969/j.issn.2096-1553.2017.3.009
Tag identification of flue-cured tobacco varieties by multiplex PCR technique
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JIA Meng-zhu
1
,
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SUN Jiu-zhe
2
,
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SU Dong-ying
2
,
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YANG Jin-chu
2
,
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WANG Er-bin
2
,
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XU Heng
2
,
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WANG Yong-chao
1
,
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WANG Jun-ting
1
,
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MENG Dan-dan
1
,
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MA Lin
1,,
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College of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China;
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Technology Center, China Tobacco He'nan Industrial Co., Ltd., Zhengzhou 450000, China
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Corresponding author:
MA Lin,
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Received Date:
2016-10-13
Available Online:
2017-05-15
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Abstract
With 12 varieties of flue-cured tobacco as a template,the conditions for primer concentration and annealing temperature for 6 pairs of SCAR primers were optimized. Multiplex PCR fingerprints and identification routes of 12 flue-cured tobacco varieties were established by multiplex PCR. The results showed that multiplex PCR had the advantages of high efficiency, high speed, time saving and low cost compared with ordinary PCR. Six pairs of SCAR primers had good stability and specific band specificity, which could distinguish 12 flue-cured tobacco varieties accurately.
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Proportional views
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