JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 32 Issue 3
May 2017
Article Contents
JIA Meng-zhu, SUN Jiu-zhe, SU Dong-ying, et al. Tag identification of flue-cured tobacco varieties by multiplex PCR technique[J]. Journal of Light Industry, 2017, 32(3): 51-57. doi: 10.3969/j.issn.2096-1553.2017.3.009
Citation: JIA Meng-zhu, SUN Jiu-zhe, SU Dong-ying, et al. Tag identification of flue-cured tobacco varieties by multiplex PCR technique[J]. Journal of Light Industry, 2017, 32(3): 51-57. doi: 10.3969/j.issn.2096-1553.2017.3.009 shu

Tag identification of flue-cured tobacco varieties by multiplex PCR technique

  • Corresponding author: MA Lin, 
  • Received Date: 2016-10-13
    Available Online: 2017-05-15
  • With 12 varieties of flue-cured tobacco as a template,the conditions for primer concentration and annealing temperature for 6 pairs of SCAR primers were optimized. Multiplex PCR fingerprints and identification routes of 12 flue-cured tobacco varieties were established by multiplex PCR. The results showed that multiplex PCR had the advantages of high efficiency, high speed, time saving and low cost compared with ordinary PCR. Six pairs of SCAR primers had good stability and specific band specificity, which could distinguish 12 flue-cured tobacco varieties accurately.
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