QIAN Cun, WANG Yun-long, MING Liang, et al. Study on quantitative detection of tPSA and fPSA by fluorescent chromatography[J]. Journal of Light Industry, 2017, 32(4): 37-42. doi: 10.3969/j.issn.2096-1553.2017.4.006
Citation:
QIAN Cun, WANG Yun-long, MING Liang, et al. Study on quantitative detection of tPSA and fPSA by fluorescent chromatography[J]. Journal of Light Industry, 2017, 32(4): 37-42.
doi:
10.3969/j.issn.2096-1553.2017.4.006
Study on quantitative detection of tPSA and fPSA by fluorescent chromatography
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QIAN Cun
1
,
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WANG Yun-long
1,2,3,,
,
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MING Liang
1
,
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LI Yu-lin
2
,
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WANG Ji-chuang
2
,
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CHENG Lei
2
,
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CHENG Chun-jie
3
,
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GAO Yu-hong
3
,
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YAN Sheng-hui
3
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The First Affiliated Hosptial of Zhengzhou University, Zhengzhou 450000, China;
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Zhengzhou Biopharmaceutical Key Laboratory, He'nan Bioengineering Technology Research Center, Zhengzhou 450000, China;
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Zhengzhou Technical College, Zhengzhou 450001, China
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Corresponding author:
WANG Yun-long,
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Received Date:
2017-03-22
Available Online:
2017-07-15
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Abstract
Conjugating the EDC activated fluorescent microspheres with antibody to prepare the fluorescence chromatographic strip for simultaneous detection of tPSA and fPSA. The performance analysis and methodological comparison for the test strip were further performed. The results showed that the sensitivity for PSA and fPSA detection by the test strip were 0.08 ng/mL and 0.06 ng/mL, respectively. The coefficient of variation within and between groups was less than 10% and 15%, respectively. The recovery rates ranged from 95% to 105%. The specificity of the test strip was well. The results revealed a good correlation between self-prepared test strip and electrochemiluminescence detection, which could satisfy the needs of aided diagnosis of small-scale hospitals.
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References
-
[1]
WANG M C,PAPSIDERO L D,KURIYAMA M,et al.Prostate antigen: A new potential marker for prostatic cancer[J].Prostate,1981(2):89.
-
[2]
胡海强,朱雪萍,宋捷,等.血清前列腺特异性抗原对前列腺良恶性肿瘤诊断的临床应用[J].国际检验医学杂志,2017(1):24.
-
[3]
刘亚巍,蔡小兵,王飞,等.前列腺特异性抗原比值在前列腺癌诊断灰区的临床意义[J].中日友好医院学报,2012,26(1):12.
-
[4]
BJORK T,BJARTELL A,ABRAHAMSSON P A,et al.Alpha-1-antichy-motrypsin production in PSA-producing cell is common inprostate cancer but rare in benign prostatic hyperplasia [J].Urology,1994,43:427.
-
[5]
姜韬.TPSA、F/T值及PSAD在前列腺癌诊断中的价值[D].天津:天津医科大学,2011.
-
[6]
CHEN R,XIE L,CAI X,et al.Percent free prostate-specific antigen for prostate cancer diagnosis in Chinese men with a PSA of 4.0~10.0 ng/mL:Results from the Chinese prostate cancer consortium[J].Asian Journal of Urology,2015,2(2):107.
-
[7]
谢冲,唐群业,王国民.Luminex液相芯片同时检测 tPSA和fPSA方法的建立与评价[J].复旦学报(医学版),2010,37(4):391.
-
[8]
任志奇,吴英松,刘天才.新型免疫层析技术的研究进展[J].广东医学,2013,34(2):312.
-
[9]
王云龙.免疫诊断试剂实用技术[M].北京:海洋出版社,2009.
-
[10]
吴彤.牛布鲁氏菌病荧光标记免疫层析试纸条检测技术研究[D].呼和浩特:内蒙古农业大学,2016.
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Proportional views
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