云南三川火腿加工中微生物区系变化规律研究

胡永金; 陈红; 薛桥丽; 奎梦漪; 付晓萍; 朱仁俊; 普岳红; 黄启超

doi:10.3969/j.issn.2096-1553.2017.5.002

CN 41-1437/TS ISSN 2096-1553

云南三川火腿加工中微生物区系变化规律研究

胡永金 , 陈红 , 薛桥丽 , 奎梦漪 , 付晓萍 , 朱仁俊 , 普岳红 , 黄启超

胡永金, 陈红, 薛桥丽, 等. 云南三川火腿加工中微生物区系变化规律研究[J]. 轻工学报, 2017, 32(5): 8-15. doi: 10.3969/j.issn.2096-1553.2017.5.002

引用本文: 胡永金, 陈红, 薛桥丽, 等. 云南三川火腿加工中微生物区系变化规律研究[J]. 轻工学报, 2017, 32(5): 8-15. doi: 10.3969/j.issn.2096-1553.2017.5.002

HU Yong-jin, CHEN Hong, XUE Qiao-li, et al. Study on the changes law of microbial flora during Yunnan Sanchuan ham processing[J]. Journal of Light Industry, 2017, 32(5): 8-15. doi: 10.3969/j.issn.2096-1553.2017.5.002

Citation: HU Yong-jin, CHEN Hong, XUE Qiao-li, et al. Study on the changes law of microbial flora during Yunnan Sanchuan ham processing[J]. Journal of Light Industry, 2017, 32(5): 8-15. doi: 10.3969/j.issn.2096-1553.2017.5.002

云南三川火腿加工中微生物区系变化规律研究

云南农业大学食品科学技术学院, 云南昆明 650201;
云南农业大学图书馆, 云南昆明 650201;
云南大学生命科学学院, 云南昆明 650500;
云南省畜产品加工工程技术研究中心, 云南昆明 650201
基金项目: 国家自然科学基金项目（31460445）
中图分类号: TS251.5

Study on the changes law of microbial flora during Yunnan Sanchuan ham processing

College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
Library, Yunnan Agricultural University, Kunming 650201, China;
School of Life Sciences, Yunnan University, Kunming 650500, China;
Research Center of Livestock Product Processing and Engineering Technology of Yunnan Province, Kunming 650201, China
Received Date: 2017-04-26
Available Online: 2017-09-15
CLC number: TS251.5

摘要: 对传统工艺制作的三川火腿加工中微生物区系的动态变化规律进行了研究，并通过形态学和生理生化试验对优势菌进行分离鉴定.结果表明，腌制期、风干期和焐灰期分别为三川火腿表面和内部细菌和葡萄球菌数量的增长期、稳定期和减少期；除腌制初期外，火腿内部假单胞菌数量均高于其表面，且在整个加工过程中由于微生物之间的竞争性关系呈逐渐降低趋势；腌制后期为火腿表面和内部霉菌数量的急剧增长期，但在焐灰期骤减，直至未检出.三川火腿优势菌为马尾葡萄球菌和模仿葡萄球菌.加工过程中加强对腌制期和风干期的时间与温度控制，有利于形成前体物质，从而更好地促进焐灰期火腿优良风味的形成.
- 三川火腿 /
- 微生物区系 /
- 生理生化鉴定
Abstract: The changes law of microbial flora during Sanchuan ham processing was studied, and the dominant bacteria was further identified by morphological and physiological and biochemical tests. The results showed that the curing period, dry period and ripening period were the stages of rapid increase, steady growth and reduction of total bacteria and Staphylococcus on the surface and inside of Sanchuan ham, respectively.The total Pseudomonas count on the inside of Sanchuan ham was higher than that on the surface during the whole processing, revealing a decreasing trend due to the competitive growth between microorganisms (except for the early curing period). The total Yeast count on the surface and inside of Sanchuan ham increased during the late curing period, but substantially decreased during ripening period. The identified dominant bacteria were Staphylococcus equorum and Staphylococcus simulans.In ham processing strengthening the control of time and temperature in the curing and dry period could help form precursor substance to further the unique flavor formation of ham in ripening period.
- Sanchuan ham /
- microbial flora /
- physiological and biochemical identification
1. [1]
  李想,汪志君,于海.干腌火腿的加工工艺及其品质的影响因素[J].食品科技,2010(2):114.
2. [2]
  蒋云升,潘明,汪志君.火腿中葡萄球菌的分离、筛选及其生物学特性的研究[J].食品研究与开发,2007,28(1):12.
3. [3]
  李平兰,沈清武,吕燕妮,等.宣威火腿成熟产品中主要微生物菌相构成分析[J].中国微生态学杂志,2003,15(5):262.
4. [4]
  TOLEDANO A,JORDANO R,LÓPEZ C,et al.Proteolytic activity of lactic acid bacteria strains and fungal biota for potential use as starter cultures in dry-cured ham[J].Journal of Food Protection,2011,74(5):826.
5. [5]
  FONSECA S,CACHALDORA A,GÓMEZ M,et al.Monitoring the bacterial population dynamics during the ripening of Galician chorizo,a traditional dry fermented Spanish sausage[J].Food Microbiology,2013,33(1):77.
6. [6]
  BONOMO M G,RICCIARDI A,ZOTTA T,et al.Technological and safety characterization of coagulase-negative staphylococci from traditionally fermented sausages of Basilicata region (Southern Italy)[J].Meat Science,2009,83(1):15.
7. [7]
  黄艾祥,卢昭芬,葛长荣.云南火腿产业化发展的思考[J].肉类工业,2003(12):40.
8. [8]
  中华人民共和国卫生部.食品卫生微生物学检验肉与肉制品检验:GB/T 4789.17-2003[S].北京:中国标准出版社,2003.
9. [9]
  中华人民共和国卫生部.食品安全国家标准食品微生物学检验菌落总数测定:GB 4789.2-2010[S].北京:中国标准出版社,2010.
10. [10]
  International Organization for Standardization.Meat and meat products-Enumeration of presumptive Pseudomonas spp.:13720-2010[S].Geneva:ISO,2010.
11. [11]
  中华人民共和国卫生部.食品安全国家标准食品微生物学检验霉菌和酵母菌计数:GB 4789.15-2010[S].北京:中国标准出版社,2010.
12. [12]
  东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.
13. [13]
  GARRITY G M,BELL J A,LILBURN T G.Taxonomic outline of the procaryotes:Bergey's manual of systematic bacteriology[M].Second Edition.New York:Springer Cverlag,2003.
14. [14]
  GARCÍA-VARONA M,SANTOS E M,JAIME I,et al.Characterisation of Micrococcaceae isolated from different varieties of chorizo[J].International Journal of Food Microbiology,2000,54(3):189.
15. [15]
  于长青,任泊晓,张丽娜,等.传统发酵肉制品中葡萄球菌的分离、纯化和筛选[J].中国农学通报,2006,22(11):343.
16. [16]
  PAPAMANOLI E,KOTZEKIDOU P,TZANETAKIS N,et al.Characterization of Micrococcaceae isolated from dry fermented sausage[J].Food Microbiology,2002,19(5):441.
17. [17]
  BLAIOTTA G,PENNACCHIA C,PARENTE E,et al.Design and evaluation of specific PCR primers for rapid and reliable identification of Staphylococcus xylosus strains isolated from dry fermented sausages[J].Systematic and Applied Microbiology,2003,26(4):601.
18. [18]
  张雪梅.四川香肠生产过程中理化特性、微生物特性及产香葡萄球菌的筛选与应用[D].成都:四川农业大学,2010.
1. [1]
  李敏 , 贺姗姗 , 杨钰雯 . 改良QuEChERS方法结合超高效液相色谱测定火腿肠中杂环胺类化合物. 轻工学报, 2024, 39(5): 60-70. doi: 10.12187/2024.05.007

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沈阳化工大学材料科学与工程学院沈阳 110142

胡永金, 陈红, 薛桥丽, 等. 云南三川火腿加工中微生物区系变化规律研究[J]. 轻工学报, 2017, 32(5): 8-15. doi: 10.3969/j.issn.2096-1553.2017.5.002

引用本文: 胡永金, 陈红, 薛桥丽, 等. 云南三川火腿加工中微生物区系变化规律研究[J]. 轻工学报, 2017, 32(5): 8-15. doi: 10.3969/j.issn.2096-1553.2017.5.002

云南三川火腿加工中微生物区系变化规律研究

云南农业大学食品科学技术学院, 云南昆明 650201;
云南农业大学图书馆, 云南昆明 650201;
云南大学生命科学学院, 云南昆明 650500;
云南省畜产品加工工程技术研究中心, 云南昆明 650201

收稿日期: 2017-04-26
网络出版日期: 2017-09-15

基金项目: 国家自然科学基金项目（31460445）

关键词:

摘要: 对传统工艺制作的三川火腿加工中微生物区系的动态变化规律进行了研究，并通过形态学和生理生化试验对优势菌进行分离鉴定.结果表明，腌制期、风干期和焐灰期分别为三川火腿表面和内部细菌和葡萄球菌数量的增长期、稳定期和减少期；除腌制初期外，火腿内部假单胞菌数量均高于其表面，且在整个加工过程中由于微生物之间的竞争性关系呈逐渐降低趋势；腌制后期为火腿表面和内部霉菌数量的急剧增长期，但在焐灰期骤减，直至未检出.三川火腿优势菌为马尾葡萄球菌和模仿葡萄球菌.加工过程中加强对腌制期和风干期的时间与温度控制，有利于形成前体物质，从而更好地促进焐灰期火腿优良风味的形成.

English Abstract

Study on the changes law of microbial flora during Yunnan Sanchuan ham processing

College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
Library, Yunnan Agricultural University, Kunming 650201, China;
School of Life Sciences, Yunnan University, Kunming 650500, China;
Research Center of Livestock Product Processing and Engineering Technology of Yunnan Province, Kunming 650201, China

Received Date: 2017-04-26
Available Online: 2017-09-15

Abstract: The changes law of microbial flora during Sanchuan ham processing was studied, and the dominant bacteria was further identified by morphological and physiological and biochemical tests. The results showed that the curing period, dry period and ripening period were the stages of rapid increase, steady growth and reduction of total bacteria and Staphylococcus on the surface and inside of Sanchuan ham, respectively.The total Pseudomonas count on the inside of Sanchuan ham was higher than that on the surface during the whole processing, revealing a decreasing trend due to the competitive growth between microorganisms (except for the early curing period). The total Yeast count on the surface and inside of Sanchuan ham increased during the late curing period, but substantially decreased during ripening period. The identified dominant bacteria were Staphylococcus equorum and Staphylococcus simulans.In ham processing strengthening the control of time and temperature in the curing and dry period could help form precursor substance to further the unique flavor formation of ham in ripening period.