JOURNAL OF LIGHT INDUSTRY

CN 41-1437/TS  ISSN 2096-1553

Volume 32 Issue 6
November 2017
Article Contents
HE Pei-xin, LI Cong-cong, HU Xiao-long, et al. Component analysis of volatile metabolites of culturable Clostridium spp. isolated from pit muds of Luzhou-flavor liquor based on HS-SPME-GC-MS[J]. Journal of Light Industry, 2017, 32(6): 1-11. doi: 10.3969/j.issn.2096-1553.2017.6.001
Citation: HE Pei-xin, LI Cong-cong, HU Xiao-long, et al. Component analysis of volatile metabolites of culturable Clostridium spp. isolated from pit muds of Luzhou-flavor liquor based on HS-SPME-GC-MS[J]. Journal of Light Industry, 2017, 32(6): 1-11. doi: 10.3969/j.issn.2096-1553.2017.6.001 shu

Component analysis of volatile metabolites of culturable Clostridium spp. isolated from pit muds of Luzhou-flavor liquor based on HS-SPME-GC-MS

  • Corresponding author: HU Xiao-long, 
  • Received Date: 2017-08-30
    Available Online: 2017-11-15
  • A total of 88 pure cultures was obtained from the pit muds of one wine corporation following separation and purification culture. The 75 strains of bacteria were assigned into 8 phylogenetic clusters (cluster Ⅰ-Ⅷ) based on the analysis of 16S rRNA gene sequences and phylogenetic tree. In them, the most similar bacteria were C. tyrobutyricum, C. butyricum, C. indolis, C. acetobutylicum, C. bifermentans, C. sporogenes, C. cadaveris and C. kluyveri, respectively. The volatile metabolites of 8 representative Clostridium spp. were analyzed using headspace solid-phase microextraction (HS-SPME) method followed by gas chromatography-mass spectrometry (GC-MS). A total of 75 volatile metabolites was detected, including 23 alcohols,20 acids,14 esters,2 aldehydes,2 ketones and other 14 volatile substances. The principal component analysis revealed that the representative strain RL1 (C. tyrobutyricum) and EL7 (C. butyricum) were mainly related to the generation of acetic acid and butyric acid, and EL46 (C. kluyveri), RL20 (C. acetobutylicum) and RL10 (C. indolis) mainly contributed to the formation of caproic acid and ethyl hexanoate, and EL12 (C. bifermentans) and RL23 (C. sporogenes) were mainly associated with the S-methyl acetate, 1-Decyl alcohol and octanol, and EL43 (C. cadaveris) was mainly correlated to the biosynthesis of various esters.
  • 加载中
    1. [1]

      FAN W L,QIAN M C.Characterization of aroma compounds of Chinese "Wuliangye" and "Jiannanchun" liquors by aroma extract dilution analysis[J].Journal of Agricultural and Food Chemistry,2006,54(7):2695.

    2. [2] ZHENG J,LIANG R,WU C,et al.Discrimination of different kinds of Luzhou-flavor raw liquors based on their volatile features[J].Food Research International,2013,56:77.

    3. [3] FAN W,QIAN M C.Identification of aroma compounds in Chinese "Yanghe Daqu" liquor by normal phase chromatography fractionation followed by gas chromatography/olfactometry[J].Flavour and Fragrance Journal,2006,21(2):333.

    4. [4] HU X L,DU H,XU Y.Identification and quantification of the caproic acid-producing bacterium Clostridium kluyveri in the fermentation of pit mud used for Chinese strong-aroma type liquor production[J].International Journal of Food Microbiology,2015,214:116.

    5. [5] ZHENG X W,TABRIZI M R,NOUT M J R,et al.Daqu-A traditional Chinese liquor fermentation starter[J].Journal of the Institute of Brewing,2011,117(1):82.

    6. [6] HU X,DU H,REN C,et al.Illuminating anaerobic microbial community and cooccurrence patterns across a quality gradient in Chinese liquor fermentation pit muds[J].Applied and Environmental Microbiology,2016,82(8):2506.

    7. [7] TAO Y,LI J,RUI J,et al.Prokaryotic communities in pit mud from different-aged cellars used for the production of Chinese strong-flavored liquor[J].Applied and Environmental Microbiology,2014,80(7):2254.

    8. [8] SJÖBLOM M,MATSAKAS L,CHRISTAKOPOULOS P,et al.Production of butyric acid by Clostridium tyrobutyricum,(ATCC25755) using sweet sorghum stalks and beet molasses[J].Industrial Crops and Products,2015,74:535.

    9. [9] CAI G,JIN B,SAINT C,et al.Genetic manipulation of butyrate formation pathways in Clostridium butyricum[J].Journal of Biotechnology,2011,155(3):269.

    10. [10]

      [10] LI H G,LI W,GU Q Y,et al.Acetone,butanol,and ethanol production from cane molasses using Clostridium beijerinckii mutant obtained by combined low-energy ion beam implantation and N-methyl-N-nitro-N-nitrosoguanidine induction[J].Bioresource Technology,2013,137:254.

    11. [11]

      [11] ZHENG J,LIANG R,ZHANG L,et al.Characterization of microbial communities in strong aromatic liquor fermentation pit muds of different ages assessed by combined DGGE and PLFA analyses[J].Food Research International,2013,54(1):660.

    12. [12]

      [12] ELSDEN S R,HILTON M G,WALLER J M.The end products of the metabolism of aromatic amino acids by Clostridia[J].Archives of Microbiology,1976,107(3):283.

    13. [13]

      [15] WANG M Y,ZHANG W X.Analysis of microbial community structure in pit mud from two Chinese Luzhou-flavor liquor producing areas[J].Microbiology China,2014,41(8):1498.

    14. [14]

      [16] WANG C,CHEN Q,WANG Q,et al.Long-term batch brewing accumulates adaptive microbes,which comprehensively produce more flavorful Chinese liquors[J].Food Research International,2014,62:894.

    15. [15]

      [17] HU X L,WANG H Y,WU Q,et al.Development,validation and application of specific primers for analyzing the clostridial diversity in dark fermentation pit mud by PCR-DGGE[J].Bioresource Technology,2014,163:40.

    16. [16]

      [18] STACKEBRANDT E,EBERS J.Taxonomic parameters revisited:tarnished gold standards[J].Microbiology Today,2006,33(4):152.

    17. [17]

      [19] LIU C,HUANG D,LIU L,et al.Clostridium swellfunianum sp.nov.,a novel anaerobic bacterium isolated from the pit mud of Chinese Luzhou-flavor liquor production[J].Antonie Van Leeuwenhoek,2014,106(4):817.

Article Metrics

Article views(9098) PDF downloads(94) Cited by()

Ralated
    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return