不同酶解条件对烟叶蛋白水解度和抗氧化性的影响
Effects of different enzymolysis conditions on the protein hydrolysis degree and antioxidant activity of tobacco leaf
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摘要: 采用碱溶酸沉法从大田废弃上部烟叶中提取蛋白质，分别使用胰蛋白酶、木瓜蛋白酶、复合蛋白酶对烟叶蛋白进行酶解，研究不同酶解条件对烟叶蛋白水解度和烟叶蛋白多肽对DPPH清除率的影响.结果表明：蛋白多肽水解度最优的酶解条件与蛋白多肽对DPPH清除率最高的酶解条件不同.以使用木瓜蛋白酶为例，烟叶蛋白水解度最优酶解条件为，酶解温度50℃，酶解时间为0.5 h，加酶量为底物的3%，酶解pH=8；DPPH清除率最优酶解条件为，酶解温度40℃，酶解时间2 h，加酶量为底物质量的3%，pH=7.这说明蛋白多肽的水解度高低与其抗氧化性并非正相关，蛋白多肽既可以作为美拉德反应的底物，也可以作为抗氧化剂，可针对不同的应用目的，选择相应的最优酶解条件.Abstract: Tobacco leaf protein was extracted by alkali-solution and acid-isolation method from upper leaves in the field. The tobacco protein was hydrolyzed using trypsin, papain or protamex, respectively. The effect of different enzymolysis conditions on leaf protein hydrolysis degree and the DPPH clearance rate of tobacco leaf protein polypeptide were investigated. The results showed that the optimal enzymolysis conditions of protein peptide were different with those of the highest DPPH clearance rate of protein polypeptide. Using papain as an example, the optimal hydrolysis conditions for tobacco protein hydrolysis degree were enzymolysis temperature 50℃, hydrolysis time 0.5 h, enzyme amount 3%, pH=8. The optimal hydrolysis conditions for DPPH clearance rate were temperature 40℃, enzymolysis time 2 h, enzyme amount 3%, pH=7. This indicated that the degree of hydrolysis of protein polypeptide was not positively correlated with its antioxidant activity. Protein peptide can be used as substrate for Maillard reaction or antioxidant, and it may be chosen corresponding optimal enzymatic conditions for different purposes.
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