烟叶中西柏三烯-4,6-二醇降解产香菌的分离与鉴定
Isolation and identification of a cembratriene-4,6-diol degradation and aroma producing strain from tobacco leaf
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摘要: 采取稀释涂布平板法和平板划线法,从调制陈化后的烟叶中分离得到一株高效降解西柏三烯-4,6-二醇的菌株YI1-3.以西柏三烯-4,6-二醇为唯一碳源,该菌株降解产物主要为金合欢醛(8.57%).根据形态特征和16S rDNA系统进化树分析,初步鉴定菌株YI1-3为新鞘氨醇杆菌(Novosphingobium panipatense).该菌株在含有西柏三烯-4,6-二醇发酵培养基中培养66 h后,底物降解率达到75.58%.
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关键词:
- 西柏三烯-4,6-二醇 /
- 分离鉴定 /
- 降解率 /
- 金合欢醛 /
- 新鞘氨醇杆菌
Abstract: A high efficient cembratriene-4,6-diol degradation strain YI1-3 was isolated from tobacco leaf with spread plate and streak plate methods. The cembratriene-4,6-diol was the only carbon source, and the major degradation products were farnesal (8.57%). This strain YI1-3 was identified preliminarily as Novosphingobium panipatense through the analysis of classical morphology and 16S rDNA sequence of phylogenetic tree. The strain was cultured in the fementation medium containing cembratriene-4,6-diol, the degradation rate of substrates reached 74.58% after 66 h. -
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[1]
ENZELL C R,WAHLBERG I,AASEN A J.Isoprenoids and alkaloids of tobacco[M]//Fortschritte der Chemie Organische DNA turstoffe/Progress in the Chemistry of Organic Natural Products.Vienna:Springer,1977:1-79.
-
[2]
SEVERSON R F,JOHNSON A W,JACKSON D M.Cuticular constituents of tobacco:factors affecting their production and their role in insect and disease resistance and smoke quality[J].Rev Adv Tob Sci,1985,11:105.
-
[3]
闫克玉.烟草化学[M].郑州:郑州大学出版社,2002:1-10.
-
[4]
ENZELL C R,WAHLBERG I.Tobacco isoprenoids-precursors of important aroma constituents[J].Pure and Appl Chem,1990,62(7):1353.
-
[5]
彭玉富,王根发,刘茂林,等.不同陈化条件对烤烟烟叶香气成分变化的影响[J].河南农业大学学报,2009,43(4):349.
-
[6]
毛多斌,张槐岭,贾春晓.卷烟香味化学[M].郑州:河南科学技术出版社,1994:343-345.
-
[7]
RALDUGIN V A,FEDOROV V K,PENTEGOVA V A.Oxidative transformations of cembraned iterpenoids I-Oxidation of cembrene with chromium trioxide[J].Chem Nat Compd,1976,12(3):278.
-
[8]
RALDUGIN V A,REZVUKHIN A I,KOROTKIKH L Y,et al.Oxidative transformations of cembrane diterpenoids Ⅱ-Cembra-2,7,11-triene-4,5-diols[J].Chem Nat Compd,1977,13(1):46.
-
[9]
RALDUGIN V A,REZVUKHIN A I,KOROTKIKH L Y,et al.Oxidative transformations of cembrane diterpenoids Ⅲ-Epoxycembrenes[J].Chem Nat Compd,1977,13(4):439.
-
[10]
RALDUGIN V A,SALENKO V L,YAROSHENKO N I,et al.Oxidative transformations of cembrane diterpenoids Ⅳ-Photooxidation of cembrene[J].Chem Nat Compd,1981,17(1):54.
-
[11]
ADNARP J,CHU W L A,ENZELL C R, et al.Cheminform abstract:tobacco chemistry.Part 76.Biotransformations of tobacco isoprenoids using plant cell cultures of Tripterygium wilfordii[J].Acta Chem Scand,1993,24(42):683.
-
[12]
ADNARP J,CHU W L A,ENZELL C R, et al.Cheminform abstract:tobacco chemistry.part 77:biotransformations of a major tobacco cembratrienediol using plant cell cultures of nicotianas ylvestris[J].Acta Chem Scand,1993,24(42):689.
-
[13]
ADNARP J,CHU W L A,ENZELL C R,et al.Cheminform abstract:tobacco chemistry.Part 78.biotransformations of tobacco cembranoids using plant cell cultures of nicotian s sylvestris[J].Acta Chem Scand,1993,47:793.
-
[14]
何峰.烟草中α(β)-2,7,11-西柏三烯-4,6-二醇的分离纯化与分析及热裂解研究[D].郑州:郑州轻工业学院,2015.
-
[15]
贾蓓蕾,魏涛,黄申,等.α-胡萝卜素降解产香菌株的分离、鉴定及发酵条件优化[J].食品与发酵工业,2015,41(1):34.
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