LIU Peiyang, LIU Fang, CAI Yahui, et al. Research on the optimization of medium components of γ-polyglutamic acid-producing Bacillus amyloliquefaciens LDJ11[J]. Journal of Light Industry, 2018, 33(3): 30-38,44. doi: 10.3969/j.issn.2096-1553.2018.03.004
Citation:
LIU Peiyang, LIU Fang, CAI Yahui, et al. Research on the optimization of medium components of γ-polyglutamic acid-producing Bacillus amyloliquefaciens LDJ11[J]. Journal of Light Industry, 2018, 33(3): 30-38,44.
doi:
10.3969/j.issn.2096-1553.2018.03.004
Research on the optimization of medium components of γ-polyglutamic acid-producing Bacillus amyloliquefaciens LDJ11
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College of Life Science, He'nan Agricultural University, Zhengzhou 450002, China;
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Xinxiang Biocaro Biotech Co., Ltd., Xinxiang 453300, China
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Received Date:
2018-01-05
Available Online:
2018-05-15
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Abstract
In order to increase γ-polyglutamic acid production of Bacillus amyloliquefaciens LDJ11,based on the previous single-factor test, the Plackett-Burman test design was adopted.Three most significant factors that affected the yield of γ-polyglutamic acid were selected from the initial medium components. The steepest limbing test method was used to approach the maximum γ-polyglutamic acid production area,and then the optimization of the medium components was obtained through a three-fator and three-level Box-Behnken test.The results showed that the optimal medium components for γ-polyglutamic acid-producing Bacillus amyloliquefaciens LDJ11 were:sodium glutamate 84.53 g/L, sodium citrate 12 g/L, glycerol 30 g/L,(NH4)2SO4 21.67 g/L, CaCl2 0.07 g/L, MnSO4·H2O 0.1 g/L,MgSO4·7H2O 0.5 g/L,K2HPO4·3H2O 0.5 g/L,FeCl3·6H2O 0.06 g/L. Under this condition, the actual yield of γ-polyglutamic acid produced by Bacillus amyloliquefaciens LDJ11 was 37.048 g/L, which was 1.39 times of the initial medium component yield (26.730 g/L),and it was very close to the theoretical prediction of 36.450 g/L.
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Proportional views
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