大曲产酯化酶芽孢菌的分离鉴定及其发酵条件优化研究

侯小歌; 张杰; 孙忠科; 李童; 李学思; 闫培勋; 胡炳义

doi:10.3969/j.issn.2096-1553.2018.04.002

CN 41-1437/TS ISSN 2096-1553

大曲产酯化酶芽孢菌的分离鉴定及其发酵条件优化研究

侯小歌 , 张杰 , 孙忠科 , 李童 , 李学思 , 闫培勋 , 胡炳义

侯小歌, 张杰, 孙忠科, 等. 大曲产酯化酶芽孢菌的分离鉴定及其发酵条件优化研究[J]. 轻工学报, 2018, 33(4): 13-20. doi: 10.3969/j.issn.2096-1553.2018.04.002

引用本文: 侯小歌, 张杰, 孙忠科, 等. 大曲产酯化酶芽孢菌的分离鉴定及其发酵条件优化研究[J]. 轻工学报, 2018, 33(4): 13-20. doi: 10.3969/j.issn.2096-1553.2018.04.002

HOU Xiaoge, ZHANG Jie, SUN Zhongke, et al. Isolation, identification and fermentation conditions optimization of an esterase-producing Bacillus strain from Daqu[J]. Journal of Light Industry, 2018, 33(4): 13-20. doi: 10.3969/j.issn.2096-1553.2018.04.002

Citation: HOU Xiaoge, ZHANG Jie, SUN Zhongke, et al. Isolation, identification and fermentation conditions optimization of an esterase-producing Bacillus strain from Daqu[J]. Journal of Light Industry, 2018, 33(4): 13-20. doi: 10.3969/j.issn.2096-1553.2018.04.002

大曲产酯化酶芽孢菌的分离鉴定及其发酵条件优化研究

周口师范学院生命科学与农学学院, 河南周口 466001;
河南省宋河酒业股份有限公司, 河南鹿邑 477265
基金项目: 河南省科技厅科技攻关项目（182102110008；172102410055）；周口师范学院中青年骨干教师资助项目
中图分类号: TS261.1

Isolation, identification and fermentation conditions optimization of an esterase-producing Bacillus strain from Daqu

College of Life Science and Agriculture, Zhoukou Normal University, Zhoukou 466001, China;
He'nan Songhe Distillery Co., Ltd., Luyi 477265, China
Received Date: 2018-05-17
Available Online: 2018-07-15
CLC number: TS261.1

摘要: 以宋河大曲为分离源，采用加热和稀释涂布法富集、分离芽孢菌，利用透明圈法和摇瓶发酵法筛选产酯化酶菌株，结合形态学、生理生化特征和16S rDNA序列分析，对高产酯化酶菌株进行初步鉴定，并对其发酵条件进行优化，共分离初筛出81株产酯化酶芽孢菌，复筛出一株高产菌株SPEO-28，初步鉴定为地衣芽孢杆菌（Bacillus licheniformis）.该菌株最优产酯化酶发酵条件为：以淀粉为碳源，NH₄NO₃为氮源，初始pH值为6.0，在47℃条件下发酵3 d.在此条件下，菌株产酯化酶活力达到22.83 U/mL，比优化前提高了82.6%.
- 产酯化酶芽孢菌 /
- 大曲 /
- 酯化酶 /
- 分离鉴定 /
- 发酵条件优化
Abstract: Using Songhe Daqu as the separation source, the spores were enriched and isolated by heating and dilution coating method. The esterase-producing strains were screened by transparent circle method and flask shaking fermentation method. Combined with morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis, the high-yield esterase-producing Bacillus strain was initially identified and its fermentation conditions were optimized.A total of 81 strains of esterase-producing strains were screened and a high-yield esterase-producing strain SPEO-28 was screened out, which was initially identified as Bacillus licheniformis. The optimal esterification enzyme fermentation conditions of this strain were as follows:starch as carbon source and NH₄NO₃ as nitrogen source, initial pH value of 6.0, fermentation at 47℃ for 3 d. Under this condition, the esterase activity of the strain reached 22.83 U/mL, which was 82.6% higher than that before optimization.
- esterase-producing Bacillus strain /
- Daqu /
- esterifying enzyme /
- isolation and identification /
- fermentation condition optimization
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沈阳化工大学材料科学与工程学院沈阳 110142

侯小歌, 张杰, 孙忠科, 等. 大曲产酯化酶芽孢菌的分离鉴定及其发酵条件优化研究[J]. 轻工学报, 2018, 33(4): 13-20. doi: 10.3969/j.issn.2096-1553.2018.04.002

大曲产酯化酶芽孢菌的分离鉴定及其发酵条件优化研究

周口师范学院生命科学与农学学院, 河南周口 466001;
河南省宋河酒业股份有限公司, 河南鹿邑 477265

收稿日期: 2018-05-17
网络出版日期: 2018-07-15

基金项目: 河南省科技厅科技攻关项目（182102110008；172102410055）；周口师范学院中青年骨干教师资助项目

关键词:

摘要: 以宋河大曲为分离源，采用加热和稀释涂布法富集、分离芽孢菌，利用透明圈法和摇瓶发酵法筛选产酯化酶菌株，结合形态学、生理生化特征和16S rDNA序列分析，对高产酯化酶菌株进行初步鉴定，并对其发酵条件进行优化，共分离初筛出81株产酯化酶芽孢菌，复筛出一株高产菌株SPEO-28，初步鉴定为地衣芽孢杆菌（Bacillus licheniformis）.该菌株最优产酯化酶发酵条件为：以淀粉为碳源，NH₄NO₃为氮源，初始pH值为6.0，在47℃条件下发酵3 d.在此条件下，菌株产酯化酶活力达到22.83 U/mL，比优化前提高了82.6%.

English Abstract

Isolation, identification and fermentation conditions optimization of an esterase-producing Bacillus strain from Daqu

College of Life Science and Agriculture, Zhoukou Normal University, Zhoukou 466001, China;
He'nan Songhe Distillery Co., Ltd., Luyi 477265, China

Received Date: 2018-05-17
Available Online: 2018-07-15

Abstract: Using Songhe Daqu as the separation source, the spores were enriched and isolated by heating and dilution coating method. The esterase-producing strains were screened by transparent circle method and flask shaking fermentation method. Combined with morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis, the high-yield esterase-producing Bacillus strain was initially identified and its fermentation conditions were optimized.A total of 81 strains of esterase-producing strains were screened and a high-yield esterase-producing strain SPEO-28 was screened out, which was initially identified as Bacillus licheniformis. The optimal esterification enzyme fermentation conditions of this strain were as follows:starch as carbon source and NH₄NO₃ as nitrogen source, initial pH value of 6.0, fermentation at 47℃ for 3 d. Under this condition, the esterase activity of the strain reached 22.83 U/mL, which was 82.6% higher than that before optimization.