大曲产酯化酶芽孢菌的分离鉴定及其发酵条件优化研究

侯小歌; 张杰; 孙忠科; 李童; 李学思; 闫培勋; 胡炳义

doi:10.3969/j.issn.2096-1553.2018.04.002

July 2018

Article Contents

HOU Xiaoge, ZHANG Jie, SUN Zhongke, et al. Isolation, identification and fermentation conditions optimization of an esterase-producing Bacillus strain from Daqu[J]. Journal of Light Industry, 2018, 33(4): 13-20. doi: 10.3969/j.issn.2096-1553.2018.04.002

Citation: HOU Xiaoge, ZHANG Jie, SUN Zhongke, et al. Isolation, identification and fermentation conditions optimization of an esterase-producing Bacillus strain from Daqu[J]. Journal of Light Industry, 2018, 33(4): 13-20. doi: 10.3969/j.issn.2096-1553.2018.04.002 shu

Isolation, identification and fermentation conditions optimization of an esterase-producing Bacillus strain from Daqu

HOU Xiaoge ¹ ,
ZHANG Jie ¹ ,
SUN Zhongke ¹ ,
LI Tong ¹ ,
LI Xuesi ² ,
YAN Peixun ² ,
HU Bingyi ¹

College of Life Science and Agriculture, Zhoukou Normal University, Zhoukou 466001, China;
He'nan Songhe Distillery Co., Ltd., Luyi 477265, China

Received Date: 2018-05-17
Available Online: 2018-07-15

Abstract

Using Songhe Daqu as the separation source, the spores were enriched and isolated by heating and dilution coating method. The esterase-producing strains were screened by transparent circle method and flask shaking fermentation method. Combined with morphology, physiological and biochemical characteristics and 16S rDNA sequence analysis, the high-yield esterase-producing Bacillus strain was initially identified and its fermentation conditions were optimized.A total of 81 strains of esterase-producing strains were screened and a high-yield esterase-producing strain SPEO-28 was screened out, which was initially identified as Bacillus licheniformis. The optimal esterification enzyme fermentation conditions of this strain were as follows:starch as carbon source and NH₄NO₃ as nitrogen source, initial pH value of 6.0, fermentation at 47℃ for 3 d. Under this condition, the esterase activity of the strain reached 22.83 U/mL, which was 82.6% higher than that before optimization.
- esterase-producing Bacillus strain,
- Daqu,
- esterifying enzyme,
- isolation and identification,
- fermentation condition optimization
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沈阳化工大学材料科学与工程学院沈阳 110142

Isolation, identification and fermentation conditions optimization of an esterase-producing Bacillus strain from Daqu

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